Restriction map for
pFN21AE3608
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbrPI BclI BglII BlnI
BsaBI BsiEI BspDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2608 GACGTC GACGT^C
AflII 904 CTTAAG C^TTAAG
Alw44I 3610 GTGCAC G^TGCAC
ApaLI 3610 GTGCAC G^TGCAC
BalI 1610 TGGCCA TGG^CCA
BbeI 1214 GGCGCC GGCGC^C
BbsI 3859 GTCTTC GAAGAC(2/6)
BbvCI 493 GCTGAGG CCTCAGC(-5/-2)
BcgI 2936 GCACTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2574 GGATAC GTATCC(6/5)
BfrI 904 CTTAAG C^TTAAG
BpuAI 3859 GTCTTC GAAGAC(2/6)
BsaAI 3714 CACGTA [CT]AC^GT[GA]
BsiWI 3716 CGTACG C^GTACG
BsmI 489 GAATGC GAATGC(1/-1)
Bsp1407I 116 TGTACA T^GTACA
BspLU11I 802 ACATGT A^CATGT
BsrGI 116 TGTACA T^GTACA
BssSI 2816 CTCGTG CACGAG(-5/-1)
DraI 1104 TTTAAA TTT^AAA
DraIII 439 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3694 GACGAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1917 GAATTC G^AATTC
FseI 2389 GGCCGGCC GGCCGG^CC
HaeII 1214 GGCGCC [GA]GCGC^[CT]
KasI 1214 GGCGCC G^GCGCC
MluNI 1610 TGGCCA TGG^CCA
MscI 1610 TGGCCA TGG^CCA
NaeI 2390 GCCGGC GCC^GGC
NarI 1214 GGCGCC GG^CGCC
NdeI 1772 CATATG CA^TATG
NgoMIV 2390 GCCGGC G^CCGGC
PshAI 799 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 273 GAAGAGC GCTCTTC(1/4)
SbfI 1803 CCTGCAGG CCTGCA^GG
ScaI 224 AGTACT AGT^ACT
SexAI 344 ACCTGGT A^CC[AT]GGT
SfiI 2384 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1214 GGCGCC GGC^GCC
Sse8387I 1803 CCTGCAGG CCTGCA^GG
SspBI 116 TGTACA T^GTACA
StuI 1658 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 3032 TCCGGA T^CCGGA
3269 TCCGGA
AcsI 1917 GAATTC [GA]^AATT[CT]
3448 AAATTC
AcyI 1214 GGCGCC G[GA]^CG[CT]C
2608 GACGTC
AflIII 802 ACATGT A^C[GA][CT]GT
3713 ACACGT
AhdI 2464 GACTACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2806 GACTACCAGTC
AocI 411 CCTCAGG CC^T[ACGT]AGG
2208 CCTCAGG
ApoI 1917 GAATTC [GA]^AATT[CT]
3448 AAATTC
Asp700I 555 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3045 GAAGCGATTC
AspEI 2464 GACTACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2806 GACTACCAGTC
BglI 2151 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2385 GCCAGGCCGGC
BlpI 526 GCTCAGC GC^T[ACGT]AGC
2167 GCTCAGC
Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC
2167 GCTCAGC
BsaHI 1214 GGCGCC G[GA]^CG[CT]C
2608 GACGTC
BsaWI 3032 TCCGGA [AT]^CCGG[AT]
3269 TCCGGA
BseAI 3032 TCCGGA T^CCGGA
3269 TCCGGA
BsmBI 1390 CGTCTC CGTCTC(1/5)
3838 GAGACG
BspEI 3032 TCCGGA T^CCGGA
3269 TCCGGA
BsrDI 95 GCAATG GCAATG(2/0)
2708 GCAATG
BsrFI 1710 GCCGGT [GA]^CCGG[CT]
2390 GCCGGC
BstXI 668 CCATCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2811 CCAGTCTCGTGG
Bsu36I 411 CCTCAGG CC^T[ACGT]AGG
2208 CCTCAGG
CelII 526 GCTCAGC GC^T[ACGT]AGC
2167 GCTCAGC
Cfr10I 1710 GCCGGT [GA]^CCGG[CT]
2390 GCCGGC
Eam1105I 2464 GACTACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2806 GACTACCAGTC
Eco81I 411 CCTCAGG CC^T[ACGT]AGG
2208 CCTCAGG
EcoNI 372 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2271 CCTCCCCCAGG
Hin1I 1214 GGCGCC G[GA]^CG[CT]C
2608 GACGTC
MroI 3032 TCCGGA T^CCGGA
3269 TCCGGA
SmaI 282 CCCGGG CCC^GGG
3005 CCCGGG
SphI 1416 GCATGC GCATG^C
2697 GCATGC
Tsp509I 1918 AATT ^AATT
3449 AATT
XmaCI 282 CCCGGG C^CCGGG
3005 CCCGGG
XmaI 282 CCCGGG C^CCGGG
3005 CCCGGG
XmnI 555 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3045 GAAGCGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SalI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XhoII