Restriction map for
pFN21AE4496
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaLI AscI Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MaeII
MflI MluI MluNI MroI MscI
MspA1I MspI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Van91I XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 778 GTAGAC GT^[AC][GT]AC
AccII 58 CGCG CG^CG
AflII 648 CTTAAG C^TTAAG
AocI 486 CCTGAGG CC^T[ACGT]AGG
AseI 1024 ATTAAT AT^TAAT
AsnI 1024 ATTAAT AT^TAAT
AspI 781 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1443 TGATCA T^GATCA
BfrI 648 CTTAAG C^TTAAG
BlpI 876 GCTCAGC GC^T[ACGT]AGC
BpmI 1375 CTGGAG CTGGAG(16/14)
Bpu1102I 876 GCTCAGC GC^T[ACGT]AGC
BsaJI 252 CCAAGG C^C[ACGT][ACGT]GG
BsmAI 67 GAGAC GTCTC(1/5)
BspHI 1401 TCATGA T^CATGA
BspMI 1077 GCAGGT ACCTGC(4/8)
BssKI 885 CCAGG ^CC[ACGT]GG
BstF5I 1009 CATCC GGATG(2/0)
BstNI 885 CCAGG CC^[AT]GG
BstUI 58 CGCG CG^CG
Bsu36I 486 CCTGAGG CC^T[ACGT]AGG
CelII 876 GCTCAGC GC^T[ACGT]AGC
EarI 1455 CTCTTC CTCTTC(1/4)
Eco81I 486 CCTGAGG CC^T[ACGT]AGG
EcoRI 257 GAATTC G^AATTC
EcoRII 885 CCAGG ^CC[AT]GG
EcoRV 163 GATATC GAT^ATC
EcoT14I 252 CCAAGG C^C[AT][AT]GG
FbaI 1443 TGATCA T^GATCA
FokI 1009 CATCC GGATG(9/13)
HincII 673 GTCAAC GT[CT]^[GA]AC
HindII 673 GTCAAC GT[CT]^[GA]AC
HindIII 289 AAGCTT A^AGCTT
Ksp632I 1455 CTCTTC CTCTTC(1/4)
MfeI 317 CAATTG C^AATTG
MunI 317 CAATTG C^AATTG
MvaI 885 CCAGG CC^[AT]GG
MvnI 58 CGCG CG^CG
PflFI 781 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1024 ATTAAT AT^TAAT
PstI 1061 CTGCAG CTGCA^G
RcaI 1401 TCATGA T^CATGA
ScaI 1290 AGTACT AGT^ACT
ScrFI 885 CCAGG CC^[ACGT]GG
SfcI 1061 CTGCAG C^T[GA][CT]AG
SspI 342 AATATT AAT^ATT
StyI 252 CCAAGG C^C[AT][AT]GG
TfiI 726 GATTC G^A[AT]TC
Tth111I 781 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 216 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 39 GAATTT [GA]^AATT[CT]
257 GAATTC
ApaI 562 GGGCCC GGGCC^C
791 GGGCCC
ApoI 39 GAATTT [GA]^AATT[CT]
257 GAATTC
Asp700I 712 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1387 GAACAACTTC
BanII 562 GGGCCC G[GA]GC[CT]^C
791 GGGCCC
BmyI 562 GGGCCC G[AGT]GC[ACT]^C
791 GGGCCC
BsgI 908 CTGCAC GTGCAG(16/14)
269 GTGCAG
BsiYI 57 CCGCGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1543 CCAACAGAAGG
BslI 57 CCGCGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1543 CCAACAGAAGG
Bsp1286I 562 GGGCCC G[AGT]GC[ACT]^C
791 GGGCCC
BsrDI 423 CATTGC GCAATG(2/0)
818 GCAATG
Cac8I 525 GCAAGC GC[ACGT]^[ACGT]GC
814 GCTGGC
DraII 562 GGGCCCC [GA]G^G[ACGT]CC[CT]
791 GGGCCCT
EcoO109I 562 GGGCCCC [GA]G^G[ACGT]CC[CT]
791 GGGCCCT
PleI 175 GACTC GAGTC(4/5)
844 GACTC
TaqI 306 TCGA T^CGA
1295 TCGA
TthHB8I 306 TCGA T^CGA
1295 TCGA
XmnI 712 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1387 GAACAACTTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BfaI BpuAI BsaBI BsrI Cfr13I
DdeI DpnII Fnu4HI HaeIII HinfI
HphI ItaI MaeI MaeIII MamI
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I SfaNI SmlI Tru9I
TseI Tsp509I TspRI