Restriction map for
pFN21AE6240
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AviII AvrII BamHI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 313 AAATTC [GA]^AATT[CT]
AhdI 463 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 230 AGCT AG^CT
ApaI 322 GGGCCC GGGCC^C
ApoI 313 AAATTC [GA]^AATT[CT]
AspEI 463 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 244 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 325 CCCGAG C^[CT]CG[GA]G
BalI 272 TGGCCA TGG^CCA
BanI 28 GGCGCC G^G[CT][GA]CC
BbeI 28 GGCGCC GGCGC^C
BbrPI 496 CACGTG CAC^GTG
BcgI 284 GCAAGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 572 CCCGG CC^[GC]GG
BglI 324 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 46 CTGGAG CTGGAG(16/14)
BsaAI 496 CACGTG [CT]AC^GT[GA]
BseRI 523 GAGGAG GAGGAG(10/8)
BsmFI 143 GGGAC GGGAC(10/14)
BsoBI 325 CCCGAG C^[CT]CG[GA]G
BspMI 92 ACCTGC ACCTGC(4/8)
BsrFI 150 GCCGGC [GA]^CCGG[CT]
Cfr10I 150 GCCGGC [GA]^CCGG[CT]
Eam1105I 463 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 353 GAAGAG CTCTTC(1/4)
EcoNI 387 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 359 ATGCAT ATGCA^T
HgaI 248 GCGTC GACGC(5/10)
HphI 218 TCACC GGTGA(8/7)
KasI 28 GGCGCC G^GCGCC
Ksp632I 353 GAAGAG CTCTTC(1/4)
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MslI 264 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 159 CAGCGG C[AC]G^C[GT]G
NaeI 150 GCCGGC GCC^GGC
NarI 28 GGCGCC GG^CGCC
NciI 572 CCCGG CC^[GC]GG
NgoMIV 150 GCCGGC G^CCGGC
NsiI 359 ATGCAT ATGCA^T
PflFI 244 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 496 CACGTG CAC^GTG
PmlI 496 CACGTG CAC^GTG
PpuMI 254 AGGACCC [GA]G^G[AT]CC[CT]
SfiI 323 GGCCCGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 28 GGCGCC GGC^GCC
SmlI 202 CTCAAG C^T[CT][GA]AG
Tsp509I 314 AATT ^AATT
Tth111I 244 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 377 CCACATTTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 28 GGCGCC G[GA]^CG[CT]C
247 GGCGTC
AfaI 414 GTAC GT^AC
537 GTAC
AvaII 144 GGACC G^G[AT]CC
255 GGACC
BanII 322 GGGCCC G[GA]GC[CT]^C
526 GAGCCC
BsaHI 28 GGCGCC G[GA]^CG[CT]C
247 GGCGTC
BsmAI 451 GAGAC GTCTC(1/5)
471 GTCTC
BsrBI 85 CCGCTC CCGCTC(-3/-3)
51 GAGCGG
BsrI 25 ACTGG ACTGG(1/-1)
464 ACTGG
DpnII 43 GATC ^GATC
132 GATC
DraII 254 AGGACCC [GA]G^G[ACGT]CC[CT]
368 GGGGCCT
EaeI 155 CGGCCA [CT]^GGCC[GA]
272 TGGCCA
EcoO109I 254 AGGACCC [GA]G^G[ACGT]CC[CT]
368 GGGGCCT
HaeII 28 GGCGCC [GA]GCGC^[CT]
236 GGCGCT
HapII 151 CCGG C^CGG
573 CCGG
Hin1I 28 GGCGCC G[GA]^CG[CT]C
247 GGCGTC
HinfI 453 GACTC G^A[ACGT]TC
469 GAGTC
HpaII 151 CCGG C^CGG
573 CCGG
MaeII 79 ACGT A^CGT
497 ACGT
MaeIII 298 GTGAC ^GT[ACGT]AC
461 GTGAC
MboI 43 GATC ^GATC
132 GATC
MspI 151 CCGG C^CGG
573 CCGG
NdeII 43 GATC ^GATC
132 GATC
NlaIII 67 CATG CATG^
270 CATG
PleI 453 GACTC GAGTC(4/5)
469 GAGTC
RsaI 414 GTAC GT^AC
537 GTAC
Sau3AI 43 GATC ^GATC
132 GATC
TaqI 242 TCGA T^CGA
293 TCGA
Tsp45I 298 GTGAC ^GT[GC]AC
461 GTGAC
TthHB8I 242 TCGA T^CGA
293 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I ItaI MboII
MnlI MvaI MvnI MwoI NlaIV
Sau96I ScrFI SfaNI TseI TspRI