Restriction map for
pFN21AE4534
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbvCI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Alw44I 984 GTGCAC G^TGCAC
AlwNI 1470 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1073 CCTCAGG CC^T[ACGT]AGG
Aor51HI 559 AGCGCT AGC^GCT
ApaLI 984 GTGCAC G^TGCAC
BbrPI 135 CACGTG CAC^GTG
BclI 752 TGATCA T^GATCA
BfaI 337 CTAG C^TAG
BglI 58 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 135 CACGTG [CT]AC^GT[GA]
BsaI 1303 GGTCTC GGTCTC(1/5)
BseRI 1243 CTCCTC GAGGAG(10/8)
BsiEI 126 CGACCG CG[GA][CT]^CG
Bsp1407I 452 TGTACA T^GTACA
BspLU11I 1661 ACATGT A^CATGT
BsrGI 452 TGTACA T^GTACA
BssSI 1387 CTCGTG CACGAG(-5/-1)
BstEII 1113 GGTGACC G^GT[ACGT]ACC
BstPI 1113 GGTGACC G^GT[ACGT]ACC
Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG
Eco47III 559 AGCGCT AGC^GCT
Eco81I 1073 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1113 GGTGACC G^GT[ACGT]ACC
EcoT22I 589 ATGCAT ATGCA^T
FbaI 752 TGATCA T^GATCA
HaeII 559 AGCGCT [GA]GCGC^[CT]
MaeI 337 CTAG C^TAG
MseI 1675 TTAA T^TAA
NsiI 589 ATGCAT ATGCA^T
PmaCI 135 CACGTG CAC^GTG
PmlI 135 CACGTG CAC^GTG
PvuII 1120 CAGCTG CAG^CTG
SacI 1261 GAGCTC GAGCT^C
SapI 1263 GCTCTTC GCTCTTC(1/4)
SfiI 57 GGCCCCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 452 TGTACA T^GTACA
StuI 1787 AGGCCT AGG^CCT
TaqI 152 TCGA T^CGA
Tru9I 1675 TTAA T^TAA
Tsp509I 979 AATT ^AATT
TthHB8I 152 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 718 GTCTAC GT^[AC][GT]AC
946 GTCTAC
AflIII 134 ACACGT A^C[GA][CT]GT
1661 ACATGT
BamHI 665 GGATCC G^GATCC
1465 GGATCC
BanII 1261 GAGCTC G[GA]GC[CT]^C
1680 GGGCTC
BglII 35 AGATCT A^GATCT
872 AGATCT
BpmI 201 CTGGAG CTGGAG(16/14)
298 CTGGAG
BsmAI 1304 GTCTC GTCTC(1/5)
1405 GTCTC
BsrDI 1056 CATTGC GCAATG(2/0)
1269 CATTGC
HgaI 777 GACGC GACGC(5/10)
1557 GACGC
HincII 538 GTCAAC GT[CT]^[GA]AC
1429 GTCAAC
HindII 538 GTCAAC GT[CT]^[GA]AC
1429 GTCAAC
MaeII 136 ACGT A^CGT
197 ACGT
NcoI 1568 CCATGG C^CATGG
1631 CCATGG
PleI 841 GACTC GAGTC(4/5)
955 GACTC
PpuMI 281 GGGTCCC [GA]G^G[AT]CC[CT]
901 GGGTCCC
SfcI 528 CTACAG C^T[GA][CT]AG
948 CTACAG
TfiI 1004 GATTC G^A[AT]TC
1436 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
StyI TseI Tsp45I TspRI Van91I
XcmI XhoII XmaCI XmaI