##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AccI          131  GTCGAC               GT^[AC][GT]AC
AciI           49  GCGG                 CCGC(-3/-1)
AspHI         207  GAGCAC               G[AT]GC[AT]^C
BbvCI          64  CCTCAGC              CCTCAGC(-5/-2)
BmyI          207  GAGCAC               G[AGT]GC[ACT]^C
BseRI         121  CTCCTC               GAGGAG(10/8)
BsiHKAI       207  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      207  GAGCAC               G[AGT]GC[ACT]^C
HincII        131  GTCGAC               GT[CT]^[GA]AC
HindII        131  GTCGAC               GT[CT]^[GA]AC
HphI          116  TCACC                GGTGA(8/7)
MspA1I         47  CAGCGG               C[AC]G^C[GT]G
NlaIII        244  CATG                 CATG^
NlaIV         215  GGAACC               GG[ACGT]^[ACGT]CC
PleI          234  GACTC                GAGTC(4/5)
SalI          131  GTCGAC               G^TCGAC
SfaNI          85  GATGC                GCATC(5/9)
TaqI          132  TCGA                 T^CGA
TfiI            3  GATTC                G^A[AT]TC
Tsp509I       108  AATT                 ^AATT
TspRI          11  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       132  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BbvI           46  GCAGC                GCAGC(8/12)
              160  GCAGC               
BsaJI          53  CCCTGG               C^C[ACGT][ACGT]GG
               54  CCTGGG              
BsmAI         223  GAGAC                GTCTC(1/5)
              232  GAGAC               
BstF5I         90  CATCC                GGATG(2/0)
               84  GGATG               
Cfr13I         51  GGCCC                G^G[ACGT]CC
              201  GGGCC               
DdeI           65  CTCAG                C^T[ACGT]AG
              205  CTGAG               
FokI           90  CATCC                GGATG(9/13)
               84  GGATG               
HaeIII         51  GGCC                 GG^CC
              202  GGCC                
HinfI           3  GATTC                G^A[ACGT]TC
              234  GACTC               
Sau96I         51  GGCCC                G^G[ACGT]CC
              201  GGGCC               
TseI           46  GCAGC                G^C[AT]GC
              160  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AluI        BssKI       BstNI       EcoRII      Fnu4HI      
ItaI        MnlI        MvaI        ScrFI