Restriction map for
pFN21AE4486
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AocI 1231 CCTGAGG CC^T[ACGT]AGG
ApaI 118 GGGCCC GGGCC^C
BanI 1026 GGCACC G^G[CT][GA]CC
BbsI 301 GTCTTC GAAGAC(2/6)
BbvCI 361 GCTGAGG CCTCAGC(-5/-2)
BbvI 886 GCAGC GCAGC(8/12)
BclI 266 TGATCA T^GATCA
BpmI 877 CTGGAG CTGGAG(16/14)
BpuAI 301 GTCTTC GAAGAC(2/6)
BsaI 166 GGTCTC GGTCTC(1/5)
BspHI 1343 TCATGA T^CATGA
BstAPI 984 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 840 TTCGAA TT^CGAA
BstXI 1213 CCAAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1231 CCTGAGG CC^T[ACGT]AGG
DraIII 183 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1046 CCGTGG C^C[GA][CT]GG
Eco81I 1231 CCTGAGG CC^T[ACGT]AGG
FauI 483 GCGGG CCCGC(4/6)
FbaI 266 TGATCA T^GATCA
Fnu4HI 886 GCAGC GC^[ACGT]GC
HincII 230 GTCAAC GT[CT]^[GA]AC
HindII 230 GTCAAC GT[CT]^[GA]AC
ItaI 886 GCAGC GC^[ACGT]GC
MaeIII 508 GTCAC ^GT[ACGT]AC
MwoI 984 GCAGAAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 840 TTCGAA TT^CGAA
PpuMI 76 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 817 CAGCTG CAG^CTG
RcaI 1343 TCATGA T^CATGA
SacI 880 GAGCTC GAGCT^C
SapI 1198 GCTCTTC GCTCTTC(1/4)
ScaI 1217 AGTACT AGT^ACT
SfcI 534 CTACAG C^T[GA][CT]AG
SfuI 840 TTCGAA TT^CGAA
TseI 886 GCAGC G^C[AT]GC
Tsp45I 508 GTCAC ^GT[GC]AC
XcmI 1166 CCAAAGGGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 245 TGGCCA TGG^CCA
962 TGGCCA
BanII 118 GGGCCC G[GA]GC[CT]^C
880 GAGCTC
BfaI 280 CTAG C^TAG
805 CTAG
BsaJI 115 CCAGGG C^C[ACGT][ACGT]GG
1046 CCGTGG
BseRI 61 CTCCTC GAGGAG(10/8)
1234 GAGGAG
BsmFI 76 GGGAC GGGAC(10/14)
855 GTCCC
BsrDI 392 CATTGC GCAATG(2/0)
444 CATTGC
EaeI 245 TGGCCA [CT]^GGCC[GA]
962 TGGCCA
EarI 1199 CTCTTC CTCTTC(1/4)
378 GAAGAG
EcoRI 110 GAATTC G^AATTC
837 GAATTC
Ksp632I 1199 CTCTTC CTCTTC(1/4)
378 GAAGAG
MaeI 280 CTAG C^TAG
805 CTAG
MaeII 220 ACGT A^CGT
263 ACGT
MluNI 245 TGGCCA TGG^CCA
962 TGGCCA
MscI 245 TGGCCA TGG^CCA
962 TGGCCA
MspA1I 414 CAGCGG C[AC]G^C[GT]G
817 CAGCTG
SfaNI 1006 GATGC GCATC(5/9)
1042 GATGC
TfiI 1185 GATTC G^A[AT]TC
1477 GATTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BmyI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FokI HaeIII HinfI
HphI MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SmlI TaqI Tru9I Tsp509I
TspRI TthHB8I XhoII