Restriction map for
pFN21AE5001
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccII AclI AcyI
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BanI BbeI BbrPI
BciVI BclI BcnI BglI BlnI
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsmBI BsmI BspHI
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT22I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MluI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 887 GTCTAC GT^[AC][GT]AC
AccIII 3008 TCCGGA T^CCGGA
AflII 2188 CTTAAG C^TTAAG
AflIII 329 ACATGT A^C[GA][CT]GT
Alw44I 2358 GTGCAC G^TGCAC
ApaLI 2358 GTGCAC G^TGCAC
Asp700I 1687 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2782 CTCGAG C^[CT]CG[GA]G
BanII 2056 GGGCTC G[GA]GC[CT]^C
BbsI 2749 GAAGAC GAAGAC(2/6)
BcgI 2340 GCATCATAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2188 CTTAAG C^TTAAG
BpuAI 2749 GAAGAC GAAGAC(2/6)
BsaWI 3008 TCCGGA [AT]^CCGG[AT]
BseAI 3008 TCCGGA T^CCGGA
BsgI 150 CTGCAC GTGCAG(16/14)
BsoBI 2782 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2489 TGTACA T^GTACA
BspDI 280 ATCGAT AT^CGAT
BspEI 3008 TCCGGA T^CCGGA
BspLU11I 329 ACATGT A^CATGT
BsrFI 1655 ACCGGC [GA]^CCGG[CT]
BsrGI 2489 TGTACA T^GTACA
BstEII 2798 GGTTACC G^GT[ACGT]ACC
BstPI 2798 GGTTACC G^GT[ACGT]ACC
BstXI 3067 CCATACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 3057 GCAAGC GC[ACGT]^[ACGT]GC
Cfr10I 1655 ACCGGC [GA]^CCGG[CT]
ClaI 280 ATCGAT AT^CGAT
DsaI 1844 CCATGG C^C[GA][CT]GG
EcoO65I 2798 GGTTACC G^GT[ACGT]ACC
EcoRV 2249 GATATC GAT^ATC
HincII 2392 GTTAAC GT[CT]^[GA]AC
HindII 2392 GTTAAC GT[CT]^[GA]AC
HpaI 2392 GTTAAC GTT^AAC
MaeII 885 ACGT A^CGT
MfeI 1030 CAATTG C^AATTG
MroI 3008 TCCGGA T^CCGGA
MspA1I 2705 CAGCTG C[AC]G^C[GT]G
MunI 1030 CAATTG C^AATTG
NcoI 1844 CCATGG C^CATGG
NdeI 1759 CATATG CA^TATG
NlaIV 2445 GGGGCC GG[ACGT]^[ACGT]CC
PaeR7I 2782 CTCGAG C^TCGAG
PpuMI 1373 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2133 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2705 CAGCTG CAG^CTG
SapI 1687 GAAGAGC GCTCTTC(1/4)
SspBI 2489 TGTACA T^GTACA
XhoI 2782 CTCGAG C^TCGAG
XmnI 1687 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 301 GTAC GT^AC
2490 GTAC
AhdI 1265 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2571 GACAGCAAGTC
AspEI 1265 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2571 GACAGCAAGTC
BbvCI 1239 CCTCAGC CCTCAGC(-5/-2)
2115 CCTCAGC
BglII 1278 AGATCT A^GATCT
2505 AGATCT
BlpI 2728 GCTGAGC GC^T[ACGT]AGC
2844 GCTTAGC
Bpu1102I 2728 GCTGAGC GC^T[ACGT]AGC
2844 GCTTAGC
CelII 2728 GCTGAGC GC^T[ACGT]AGC
2844 GCTTAGC
CfoI 811 GCGC GCG^C
862 GCGC
DraII 431 AGGGCCT [GA]G^G[ACGT]CC[CT]
1373 AGGTCCT
Eam1105I 1265 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2571 GACAGCAAGTC
EcoO109I 431 AGGGCCT [GA]G^G[ACGT]CC[CT]
1373 AGGTCCT
EcoRI 1609 GAATTC G^AATTC
1873 GAATTC
FauI 438 CCCGC CCCGC(4/6)
3054 CCCGC
HapII 1656 CCGG C^CGG
3009 CCGG
HgaI 247 GCGTC GACGC(5/10)
2646 GCGTC
HhaI 811 GCGC GCG^C
862 GCGC
HinP1I 811 GCGC G^CGC
862 GCGC
HindIII 363 AAGCTT A^AGCTT
1158 AAGCTT
HpaII 1656 CCGG C^CGG
3009 CCGG
MspI 1656 CCGG C^CGG
3009 CCGG
MwoI 853 GCTTTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1721 GCAGTAATAGC
PflMI 948 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAGGTCCTGG
PstI 346 CTGCAG CTGCA^G
1792 CTGCAG
RsaI 301 GTAC GT^AC
2490 GTAC
Van91I 948 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAGGTCCTGG
XcmI 1590 CCAAACTCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1835 CCAGCAGGACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaII BalI BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI NdeII NlaIII NspI PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII