Restriction map for
pFN21AE2215
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AclI AcyI AflII AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HpaI KasI KspI
MamI MfeI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 722 GGTACC G^GTACC
AccIII 620 TCCGGA T^CCGGA
AcsI 247 AAATTC [GA]^AATT[CT]
AlwI 906 GATCC GGATC(4/5)
ApoI 247 AAATTC [GA]^AATT[CT]
Asp700I 735 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 722 GGTACC G^GTACC
AvaI 827 CCCGGG C^[CT]CG[GA]G
BalI 1130 TGGCCA TGG^CCA
BcgI 341 CGAGCGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 487 GTATCC GTATCC(6/5)
BclI 1166 TGATCA T^GATCA
BsaAI 1261 TACGTG [CT]AC^GT[GA]
BseAI 620 TCCGGA T^CCGGA
BsgI 703 CTGCAC GTGCAG(16/14)
BsmFI 585 GGGAC GGGAC(10/14)
BsoBI 827 CCCGGG C^[CT]CG[GA]G
BspEI 620 TCCGGA T^CCGGA
BstYI 905 AGATCC [GA]^GATC[CT]
EarI 624 GAAGAG CTCTTC(1/4)
FbaI 1166 TGATCA T^GATCA
HaeII 1269 GGCGCT [GA]GCGC^[CT]
HgaI 578 GCGTC GACGC(5/10)
HindIII 361 AAGCTT A^AGCTT
KpnI 722 GGTACC GGTAC^C
Ksp632I 624 GAAGAG CTCTTC(1/4)
MflI 905 AGATCC [GA]^GATC[CT]
MluI 809 ACGCGT A^CGCGT
MluNI 1130 TGGCCA TGG^CCA
MroI 620 TCCGGA T^CCGGA
MscI 1130 TGGCCA TGG^CCA
MseI 764 TTAA T^TAA
MslI 41 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 116 GCCGGC GCC^GGC
NgoMIV 116 GCCGGC G^CCGGC
PflMI 540 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1291 GAGCTC GAGCT^C
SmaI 827 CCCGGG CCC^GGG
Tru9I 764 TTAA T^TAA
Van91I 540 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1211 CCAAAGGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 905 AGATCC [GA]^GATC[CT]
XmaCI 827 CCCGGG C^CCGGG
XmaI 827 CCCGGG C^CCGGG
XmnI 735 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 99 GTCGAC GT^[AC][GT]AC
1123 GTCGAC
AccII 810 CGCG CG^CG
1305 CGCG
AfaI 723 GTAC GT^AC
1260 GTAC
AflIII 809 ACGCGT A^C[GA][CT]GT
1262 ACGTGT
AgeI 767 ACCGGT A^CCGGT
938 ACCGGT
ApaI 595 GGGCCC GGGCC^C
830 GGGCCC
AvaII 918 GGACC G^G[AT]CC
936 GGACC
BglI 116 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
457 GCCAAGACGGC
BsaI 1280 GAGACC GGTCTC(1/5)
1084 GGTCTC
BseRI 658 GAGGAG GAGGAG(10/8)
682 GAGGAG
BsmAI 1280 GAGAC GTCTC(1/5)
1085 GTCTC
BstEII 846 GGTCACC G^GT[ACGT]ACC
1231 GGTGACC
BstPI 846 GGTCACC G^GT[ACGT]ACC
1231 GGTGACC
BstUI 810 CGCG CG^CG
1305 CGCG
BstXI 974 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1073 CCAAGGCACTGG
CfoI 114 GCGC GCG^C
1270 GCGC
DrdI 571 GACGACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
879 GACAGAGATGTC
EcoNI 534 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
599 CCTACCGGAGG
EcoO65I 846 GGTCACC G^GT[ACGT]ACC
1231 GGTGACC
FauI 497 GCGGG CCCGC(4/6)
640 GCGGG
HhaI 114 GCGC GCG^C
1270 GCGC
HinP1I 114 GCGC G^CGC
1270 GCGC
HincII 99 GTCGAC GT[CT]^[GA]AC
1123 GTCGAC
HindII 99 GTCGAC GT[CT]^[GA]AC
1123 GTCGAC
MvnI 810 CGCG CG^CG
1305 CGCG
NcoI 93 CCATGG C^CATGG
1133 CCATGG
PinAI 767 ACCGGT A^CCGGT
938 ACCGGT
PvuII 1048 CAGCTG CAG^CTG
1186 CAGCTG
RsaI 723 GTAC GT^AC
1260 GTAC
SalI 99 GTCGAC G^TCGAC
1123 GTCGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AspHI AspI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsaWI BsiEI BsiHKAI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I