Restriction map for
pFN21AE1477
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MunI MvnI MwoI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tsp45I Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Alw44I 555 GTGCAC G^TGCAC
ApaLI 555 GTGCAC G^TGCAC
AspHI 555 GTGCAC G[AT]GC[AT]^C
AvaI 992 CTCGAG C^[CT]CG[GA]G
AvaII 527 GGACC G^G[AT]CC
BanII 755 GAGCCC G[GA]GC[CT]^C
BbsI 291 GAAGAC GAAGAC(2/6)
BclI 300 TGATCA T^GATCA
BglII 401 AGATCT A^GATCT
BpmI 745 CTGGAG CTGGAG(16/14)
BpuAI 291 GAAGAC GAAGAC(2/6)
BsaI 387 GGTCTC GGTCTC(1/5)
BsgI 62 GTGCAG GTGCAG(16/14)
BsiHKAI 555 GTGCAC G[AT]GC[AT]^C
BsmI 84 GAATGC GAATGC(1/-1)
BsoBI 992 CTCGAG C^[CT]CG[GA]G
BsrI 587 CCAGT ACTGG(1/-1)
BstF5I 841 CATCC GGATG(2/0)
BstYI 401 AGATCT [GA]^GATC[CT]
DraI 259 TTTAAA TTT^AAA
DsaI 1014 CCATGG C^C[GA][CT]GG
EarI 927 GAAGAG CTCTTC(1/4)
EcoRV 307 GATATC GAT^ATC
FbaI 300 TGATCA T^GATCA
FokI 841 CATCC GGATG(9/13)
HapII 786 CCGG C^CGG
HpaII 786 CCGG C^CGG
Ksp632I 927 GAAGAG CTCTTC(1/4)
MflI 401 AGATCT [GA]^GATC[CT]
MslI 518 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 603 CAGCTG C[AC]G^C[GT]G
MspI 786 CCGG C^CGG
NcoI 1014 CCATGG C^CATGG
NdeI 645 CATATG CA^TATG
PaeR7I 992 CTCGAG C^TCGAG
PflMI 850 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 723 GAGTC GAGTC(4/5)
PvuII 603 CAGCTG CAG^CTG
SexAI 855 ACCTGGT A^CC[AT]GGT
SfaNI 285 GCATC GCATC(5/9)
SmlI 992 CTCGAG C^T[CT][GA]AG
SspI 360 AATATT AAT^ATT
StuI 931 AGGCCT AGG^CCT
Van91I 850 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 397 CCAAAGATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 992 CTCGAG C^TCGAG
XhoII 401 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 892 CCGC CCGC(-3/-1)
976 CCGC
BanI 103 GGTGCC G^G[CT][GA]CC
873 GGCACC
BbvI 659 GCAGC GCAGC(8/12)
1021 GCAGC
BfaI 137 CTAG C^TAG
878 CTAG
BsmFI 887 GTCCC GGGAC(10/14)
1038 GTCCC
Cac8I 25 GCCAGC GC[ACGT]^[ACGT]GC
974 GCCCGC
DpnII 301 GATC ^GATC
402 GATC
FauI 891 CCCGC CCCGC(4/6)
975 CCCGC
Fnu4HI 659 GCAGC GC^[ACGT]GC
1021 GCAGC
HphI 701 TCACC GGTGA(8/7)
960 TCACC
ItaI 659 GCAGC GC^[ACGT]GC
1021 GCAGC
MaeI 137 CTAG C^TAG
878 CTAG
MboI 301 GATC ^GATC
402 GATC
NdeII 301 GATC ^GATC
402 GATC
NlaIII 11 CATG CATG^
1015 CATG
Sau3AI 301 GATC ^GATC
402 GATC
TaqI 593 TCGA T^CGA
993 TCGA
TseI 659 GCAGC G^C[AT]GC
1021 GCAGC
TspRI 588 CAGTG CA[GC]TG[ACGT][ACGT]^
683 CACTG
TthHB8I 593 TCGA T^CGA
993 TCGA
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI ApoI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI Cfr13I DdeI EcoRII
EcoT14I HaeIII HinfI MboII MnlI
MseI MvaI NlaIV Sau96I ScrFI
StyI TfiI Tru9I Tsp509I