Restriction map for
pFN21AE3756
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BsrBI
BsrDI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwNI 1045 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 654 GGCGCGCC GG^CGCGCC
AvaI 508 CTCGAG C^[CT]CG[GA]G
BanII 106 GGGCTC G[GA]GC[CT]^C
BfaI 124 CTAG C^TAG
BsaBI 336 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 15 CTCCTC GAGGAG(10/8)
BsiEI 816 CGATCG CG[GA][CT]^CG
BsmAI 233 GTCTC GTCTC(1/5)
BsmBI 232 CGTCTC CGTCTC(1/5)
BsoBI 508 CTCGAG C^[CT]CG[GA]G
Bsp1407I 176 TGTACA T^GTACA
BsrFI 856 GCCGGC [GA]^CCGG[CT]
BsrGI 176 TGTACA T^GTACA
BssSI 571 CTCGTG CACGAG(-5/-1)
BstAPI 372 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 856 GCCGGC [GA]^CCGG[CT]
DpnII 817 GATC ^GATC
EarI 208 CTCTTC CTCTTC(1/4)
Ksp632I 208 CTCTTC CTCTTC(1/4)
MaeI 124 CTAG C^TAG
MaeII 347 ACGT A^CGT
MamI 336 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 817 GATC ^GATC
NaeI 856 GCCGGC GCC^GGC
NdeII 817 GATC ^GATC
NgoMIV 856 GCCGGC G^CCGGC
NheI 123 GCTAGC G^CTAGC
PaeR7I 508 CTCGAG C^TCGAG
PvuI 816 CGATCG CGAT^CG
PvuII 321 CAGCTG CAG^CTG
SapI 207 GCTCTTC GCTCTTC(1/4)
Sau3AI 817 GATC ^GATC
SfcI 84 CTACAG C^T[GA][CT]AG
SmlI 508 CTCGAG C^T[CT][GA]AG
SspBI 176 TGTACA T^GTACA
TfiI 1105 GATTC G^A[AT]TC
Tsp45I 1009 GTCAC ^GT[GC]AC
XcmI 863 CCAACTGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 179 ACATGT A^C[GA][CT]GT
311 ACATGT
BanI 292 GGCGCC G^G[CT][GA]CC
859 GGCGCC
BbeI 292 GGCGCC GGCGC^C
859 GGCGCC
BsgI 70 CTGCAC GTGCAG(16/14)
250 CTGCAC
BspLU11I 179 ACATGT A^CATGT
311 ACATGT
BspMI 203 ACCTGC ACCTGC(4/8)
392 ACCTGC
BsrI 257 ACTGG ACTGG(1/-1)
1040 CCAGT
BstF5I 444 CATCC GGATG(2/0)
1032 GGATG
FokI 444 CATCC GGATG(9/13)
1032 GGATG
KasI 292 GGCGCC G^GCGCC
859 GGCGCC
MaeIII 185 GTAAC ^GT[ACGT]AC
1009 GTCAC
MslI 342 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
444 CATCCTGGTG
NarI 292 GGCGCC GG^CGCC
859 GGCGCC
NlaIII 180 CATG CATG^
312 CATG
NspI 179 ACATGT [GA]CATG^[CT]
311 ACATGT
PleI 1066 GACTC GAGTC(4/5)
638 GAGTC
PpuMI 503 GGGACCT [GA]G^G[AT]CC[CT]
952 AGGACCT
SfoI 292 GGCGCC GGC^GCC
859 GGCGCC
TaqI 509 TCGA T^CGA
527 TCGA
TthHB8I 509 TCGA T^CGA
527 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I ApaLI AspHI AvaII
BbsI BbvI BcnI BmyI BpuAI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I DraII
DraIII EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV RsaI Sau96I ScrFI SfaNI
TseI TspRI