Restriction map for
pFN21AE6354
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# NO CUT ENZYMES ( 123 enzymes) #
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AclI AcsI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MluNI MscI MseI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SbfI ScaI SfaNI SfiI
SfoI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 45 GACGTC GACGT^C
AccI 292 GTCGAC GT^[AC][GT]AC
AccIII 770 TCCGGA T^CCGGA
AcyI 45 GACGTC G[GA]^CG[CT]C
AocI 339 CCTCAGG CC^T[ACGT]AGG
ApaI 578 GGGCCC GGGCC^C
AspHI 547 GTGCTC G[AT]GC[AT]^C
AvaI 129 CCCGGG C^[CT]CG[GA]G
BbrPI 960 CACGTG CAC^GTG
BsaHI 45 GACGTC G[GA]^CG[CT]C
BsaWI 770 TCCGGA [AT]^CCGG[AT]
BseAI 770 TCCGGA T^CCGGA
BsiHKAI 547 GTGCTC G[AT]GC[AT]^C
BsoBI 129 CCCGGG C^[CT]CG[GA]G
BspEI 770 TCCGGA T^CCGGA
BsrBI 303 CCGCTC CCGCTC(-3/-3)
BsrDI 426 CATTGC GCAATG(2/0)
BssHII 25 GCGCGC G^CGCGC
BstYI 1055 GGATCT [GA]^GATC[CT]
Bsu36I 339 CCTCAGG CC^T[ACGT]AGG
DrdI 160 GACCAGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1005 GAAGAG CTCTTC(1/4)
Eco81I 339 CCTCAGG CC^T[ACGT]AGG
EcoT22I 562 ATGCAT ATGCA^T
Hin1I 45 GACGTC G[GA]^CG[CT]C
HincII 292 GTCGAC GT[CT]^[GA]AC
HindII 292 GTCGAC GT[CT]^[GA]AC
HinfI 501 GAGTC G^A[ACGT]TC
Ksp632I 1005 GAAGAG CTCTTC(1/4)
MboII 1005 GAAGA GAAGA(8/7)
MflI 1055 GGATCT [GA]^GATC[CT]
MroI 770 TCCGGA T^CCGGA
MslI 406 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 562 ATGCAT ATGCA^T
NspI 755 GCATGT [GA]CATG^[CT]
PleI 501 GAGTC GAGTC(4/5)
PmaCI 960 CACGTG CAC^GTG
PmlI 960 CACGTG CAC^GTG
PstI 101 CTGCAG CTGCA^G
SalI 292 GTCGAC G^TCGAC
SapI 1005 GAAGAGC GCTCTTC(1/4)
SexAI 968 ACCTGGT A^CC[AT]GGT
SgrAI 63 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 129 CCCGGG CCC^GGG
XhoII 1055 GGATCT [GA]^GATC[CT]
XmaCI 129 CCCGGG C^CCGGG
XmaI 129 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 572 GGTACC G^GTACC
965 GGTACC
AlwI 151 GATCC GGATC(4/5)
1055 GGATC
Asp718I 572 GGTACC G^GTACC
965 GGTACC
BanII 578 GGGCCC G[GA]GC[CT]^C
934 GAGCCC
BbvCI 1134 CCTCAGC CCTCAGC(-5/-2)
93 GCTGAGG
BglI 127 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
677 GCCTGCCTGGC
BpmI 550 CTCCAG CTGGAG(16/14)
929 CTGGAG
BsaAI 661 TACGTG [CT]AC^GT[GA]
960 CACGTG
BsaI 262 GAGACC GGTCTC(1/5)
1035 GGTCTC
BseRI 625 CTCCTC GAGGAG(10/8)
1038 CTCCTC
BsgI 717 CTGCAC GTGCAG(16/14)
992 GTGCAG
BsmAI 262 GAGAC GTCTC(1/5)
1036 GTCTC
BspMI 103 GCAGGT ACCTGC(4/8)
569 GCAGGT
HaeII 35 AGCGCC [GA]GCGC^[CT]
135 GGCGCT
HgaI 522 GACGC GACGC(5/10)
167 GCGTC
HphI 981 GGTGA GGTGA(8/7)
839 TCACC
KpnI 572 GGTACC GGTAC^C
965 GGTACC
MaeIII 724 GTGAC ^GT[ACGT]AC
818 GTGAC
MspA1I 583 CAGCGG C[AC]G^C[GT]G
714 CCGCTG
NaeI 203 GCCGGC GCC^GGC
415 GCCGGC
NcoI 257 CCATGG C^CATGG
950 CCATGG
NgoMIV 203 GCCGGC G^CCGGC
415 GCCGGC
SfcI 101 CTGCAG C^T[GA][CT]AG
1030 CTACAG
TaqI 250 TCGA T^CGA
293 TCGA
Tsp45I 724 GTGAC ^GT[GC]AC
818 GTGAC
TthHB8I 250 TCGA T^CGA
293 TCGA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanI BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MboI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SmlI StyI TseI
Tsp509I TspRI