Restriction map for
pFN21AE2179
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AciI 444 CCGC CCGC(-3/-1)
AcsI 764 GAATTT [GA]^AATT[CT]
AflII 748 CTTAAG C^TTAAG
AflIII 104 ACGTGT A^C[GA][CT]GT
AlwNI 222 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 764 GAATTT [GA]^AATT[CT]
BbsI 408 GTCTTC GAAGAC(2/6)
BbvI 367 GCAGC GCAGC(8/12)
BfrI 748 CTTAAG C^TTAAG
BpuAI 408 GTCTTC GAAGAC(2/6)
BsaAI 103 TACGTG [CT]AC^GT[GA]
BsaBI 54 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 28 CCCAGCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 28 CCCAGCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 81 GTCTC GTCTC(1/5)
BsmI 480 GCATTC GAATGC(1/-1)
BspMI 43 ACCTGC ACCTGC(4/8)
BsrDI 159 CATTGC GCAATG(2/0)
BstF5I 525 CATCC GGATG(2/0)
EarI 208 GAAGAG CTCTTC(1/4)
FokI 525 CATCC GGATG(9/13)
HphI 514 GGTGA GGTGA(8/7)
Ksp632I 208 GAAGAG CTCTTC(1/4)
MaeII 104 ACGT A^CGT
MamI 54 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PleI 713 GACTC GAGTC(4/5)
PstI 76 CTGCAG CTGCA^G
ScaI 143 AGTACT AGT^ACT
TaqI 570 TCGA T^CGA
TfiI 463 GAATC G^A[AT]TC
TseI 367 GCAGC G^C[AT]GC
Tsp509I 765 AATT ^AATT
TspRI 68 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 570 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 108 GTAC GT^AC
144 GTAC
AspHI 70 GTGCTC G[AT]GC[AT]^C
787 GAGCAC
BfaI 337 CTAG C^TAG
677 CTAG
BmyI 70 GTGCTC G[AGT]GC[ACT]^C
787 GAGCAC
BsiHKAI 70 GTGCTC G[AT]GC[AT]^C
787 GAGCAC
Bsp1286I 70 GTGCTC G[AGT]GC[ACT]^C
787 GAGCAC
Cac8I 47 GCGTGC GC[ACGT]^[ACGT]GC
230 GCTGGC
DpnII 54 GATC ^GATC
60 GATC
Fnu4HI 367 GCAGC GC^[ACGT]GC
443 GCCGC
HaeIII 442 GGCC GG^CC
828 GGCC
HinfI 463 GAATC G^A[ACGT]TC
713 GACTC
ItaI 367 GCAGC GC^[ACGT]GC
443 GCCGC
MaeI 337 CTAG C^TAG
677 CTAG
MaeIII 97 GTAAC ^GT[ACGT]AC
778 GTAAC
MboI 54 GATC ^GATC
60 GATC
MseI 172 TTAA T^TAA
749 TTAA
NdeII 54 GATC ^GATC
60 GATC
RsaI 108 GTAC GT^AC
144 GTAC
Sau3AI 54 GATC ^GATC
60 GATC
SfaNI 132 GATGC GCATC(5/9)
700 GATGC
SfcI 76 CTGCAG C^T[GA][CT]AG
302 CTACAG
SmlI 504 CTTGAG C^T[CT][GA]AG
748 CTTAAG
SspI 325 AATATT AAT^ATT
738 AATATT
Tru9I 172 TTAA T^TAA
749 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI AvaII BsaJI BsmFI BsrI
BssKI BstNI Cfr13I DdeI DraII
EcoO109I EcoRII MboII MnlI MvaI
NlaIII NlaIV PpuMI Sau96I ScrFI