Restriction map for
pFN21AB6451
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeII MaeIII
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 246 GGTACC G^GTACC
AccI 638 GTCTAC GT^[AC][GT]AC
AciI 427 GCGG CCGC(-3/-1)
ApaI 121 GGGCCC GGGCC^C
Asp718I 246 GGTACC G^GTACC
BanII 121 GGGCCC G[GA]GC[CT]^C
BcnI 36 CCCGG CC^[GC]GG
BfaI 470 CTAG C^TAG
BglI 170 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 121 GGGCCC G[AGT]GC[ACT]^C
BsaBI 576 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 528 GAGACC GGTCTC(1/5)
BsgI 708 GTGCAG GTGCAG(16/14)
BsmAI 528 GAGAC GTCTC(1/5)
Bsp1286I 121 GGGCCC G[AGT]GC[ACT]^C
BsrFI 151 GCCGGC [GA]^CCGG[CT]
CfoI 262 GCGC GCG^C
Cfr10I 151 GCCGGC [GA]^CCGG[CT]
DpnII 316 GATC ^GATC
DsaI 250 CCATGG C^C[GA][CT]GG
EaeI 153 CGGCCA [CT]^GGCC[GA]
HaeII 261 AGCGCC [GA]GCGC^[CT]
HgaI 817 GACGC GACGC(5/10)
HhaI 262 GCGC GCG^C
HinP1I 262 GCGC G^CGC
HphI 33 TCACC GGTGA(8/7)
KpnI 246 GGTACC GGTAC^C
MaeI 470 CTAG C^TAG
MamI 576 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 316 GATC ^GATC
MboII 430 GAAGA GAAGA(8/7)
MslI 533 CACTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 151 GCCGGC GCC^GGC
NciI 36 CCCGG CC^[GC]GG
NcoI 250 CCATGG C^CATGG
NdeII 316 GATC ^GATC
NgoMIV 151 GCCGGC G^CCGGC
PflMI 779 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 239 CTGCAG CTGCA^G
Sau3AI 316 GATC ^GATC
SfaNI 255 GATGC GCATC(5/9)
SfcI 239 CTGCAG C^T[GA][CT]AG
SmlI 483 CTTGAG C^T[CT][GA]AG
SspI 299 AATATT AAT^ATT
Van91I 779 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 469 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 773 AAATTT [GA]^AATT[CT]
794 GAATTT
ApoI 773 AAATTT [GA]^AATT[CT]
794 GAATTT
BciVI 60 GTATCC GTATCC(6/5)
72 GTATCC
BpmI 98 CTCCAG CTGGAG(16/14)
523 CTGGAG
BseRI 65 CTCCTC GAGGAG(10/8)
590 CTCCTC
BsiYI 145 CCACCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
779 CCAAGCACTGG
BslI 145 CCACCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
779 CCAAGCACTGG
BsmFI 187 GTCCC GGGAC(10/14)
557 GTCCC
BsmI 24 GAATGC GAATGC(1/-1)
85 GCATTC
BstF5I 254 GGATG GGATG(2/0)
675 GGATG
DdeI 68 CTCAG C^T[ACGT]AG
535 CTAAG
EcoT14I 90 CCAAGG C^C[AT][AT]GG
250 CCATGG
FokI 254 GGATG GGATG(9/13)
675 GGATG
HapII 37 CCGG C^CGG
152 CCGG
HinfI 327 GATTC G^A[ACGT]TC
833 GAATC
HpaII 37 CCGG C^CGG
152 CCGG
MspI 37 CCGG C^CGG
152 CCGG
MwoI 170 GCCCAGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
920 GCACTGGCAGC
PpuMI 93 AGGACCT [GA]G^G[AT]CC[CT]
162 AGGTCCT
StyI 90 CCAAGG C^C[AT][AT]GG
250 CCATGG
TfiI 327 GATTC G^A[AT]TC
833 GAATC
XcmI 156 CCATTCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
585 CCAAGCTCCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AlwNI AvaII BanI
BbvI BsaJI BsrI BssKI BstNI
Cac8I Cfr13I DraII EcoO109I EcoRII
Fnu4HI HaeIII ItaI MnlI MseI
MspA1I MvaI NlaIII NlaIV PvuII
RsaI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI