Restriction map for
pFN21AE3586
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MscI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 95 TCCGGA T^CCGGA
AflIII 117 ACATGT A^C[GA][CT]GT
AlwNI 179 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 463 CCTCAGG CC^T[ACGT]AGG
Aor51HI 326 AGCGCT AGC^GCT
ApaI 14 GGGCCC GGGCC^C
AspI 256 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 255 GGACC G^G[AT]CC
BanII 14 GGGCCC G[GA]GC[CT]^C
BcnI 300 CCCGG CC^[GC]GG
BmyI 14 GGGCCC G[AGT]GC[ACT]^C
BsaAI 136 TACGTG [CT]AC^GT[GA]
BsaWI 95 TCCGGA [AT]^CCGG[AT]
BseAI 95 TCCGGA T^CCGGA
BseRI 223 GAGGAG GAGGAG(10/8)
BsgI 121 GTGCAG GTGCAG(16/14)
BsmAI 492 GAGAC GTCTC(1/5)
Bsp1286I 14 GGGCCC G[AGT]GC[ACT]^C
BspEI 95 TCCGGA T^CCGGA
BspLU11I 117 ACATGT A^CATGT
BsrFI 278 ACCGGC [GA]^CCGG[CT]
BstAPI 178 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 396 CATCC GGATG(2/0)
BstXI 565 CCACTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 463 CCTCAGG CC^T[ACGT]AGG
CfoI 327 GCGC GCG^C
Cfr10I 278 ACCGGC [GA]^CCGG[CT]
DraII 14 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 169 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 188 CCATGG C^C[GA][CT]GG
EaeI 44 TGGCCG [CT]^GGCC[GA]
Eco47III 326 AGCGCT AGC^GCT
Eco81I 463 CCTCAGG CC^T[ACGT]AGG
EcoNI 345 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 14 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 519 GCGGG CCCGC(4/6)
FokI 396 CATCC GGATG(9/13)
HaeII 326 AGCGCT [GA]GCGC^[CT]
HgaI 181 GACGC GACGC(5/10)
HhaI 327 GCGC GCG^C
HinP1I 327 GCGC G^CGC
MaeII 137 ACGT A^CGT
MaeIII 433 GTGAC ^GT[ACGT]AC
MroI 95 TCCGGA T^CCGGA
NciI 300 CCCGG CC^[GC]GG
NcoI 188 CCATGG C^CATGG
NspI 117 ACATGT [GA]CATG^[CT]
PflFI 256 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 36 CTGCAG CTGCA^G
SexAI 209 ACCTGGT A^CC[AT]GGT
SfaNI 533 GCATC GCATC(5/9)
SfcI 36 CTGCAG C^T[GA][CT]AG
SmlI 420 CTTGAG C^T[CT][GA]AG
Tsp45I 433 GTGAC ^GT[GC]AC
Tth111I 256 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbsI 409 GAAGAC GAAGAC(2/6)
67 GTCTTC
BpuAI 409 GAAGAC GAAGAC(2/6)
67 GTCTTC
BsrI 246 CCAGT ACTGG(1/-1)
284 CCAGT
Cac8I 239 GCCAGC GC[ACGT]^[ACGT]GC
266 GCTGGC
DdeI 314 CTGAG C^T[ACGT]AG
464 CTCAG
DpnII 55 GATC ^GATC
166 GATC
EarI 559 CTCTTC CTCTTC(1/4)
489 GAAGAG
EcoT14I 188 CCATGG C^C[AT][AT]GG
251 CCAAGG
HphI 432 GGTGA GGTGA(8/7)
341 TCACC
Ksp632I 559 CTCTTC CTCTTC(1/4)
489 GAAGAG
MboI 55 GATC ^GATC
166 GATC
MslI 114 CATACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
400 CACCAGCATG
NdeII 55 GATC ^GATC
166 GATC
NlaIV 14 GGGCCC GG[ACGT]^[ACGT]CC
15 GGCCCC
PvuII 124 CAGCTG CAG^CTG
232 CAGCTG
Sau3AI 55 GATC ^GATC
166 GATC
StyI 188 CCATGG C^C[AT][AT]GG
251 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsiYI BslI BssKI BstNI Cfr13I
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI TseI TspRI