Restriction map for
pFN21AE4480
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccIII AcsI AcyI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BanII
BbeI BbrPI BcgI BclI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 817 GTCTAC GT^[AC][GT]AC
AccII 879 CGCG CG^CG
AciI 950 CCGC CCGC(-3/-1)
AclI 584 AACGTT AA^CGTT
Alw44I 936 GTGCAC G^TGCAC
AocI 783 CCTGAGG CC^T[ACGT]AGG
ApaLI 936 GTGCAC G^TGCAC
AspHI 936 GTGCAC G[AT]GC[AT]^C
AvaI 146 CCCGAG C^[CT]CG[GA]G
AvaII 138 GGACC G^G[AT]CC
BbsI 75 GTCTTC GAAGAC(2/6)
BbvCI 918 CCTCAGC CCTCAGC(-5/-2)
BciVI 576 GGATAC GTATCC(6/5)
BcnI 873 CCCGG CC^[GC]GG
BfaI 167 CTAG C^TAG
BglII 1246 AGATCT A^GATCT
BpmI 601 CTGGAG CTGGAG(16/14)
BpuAI 75 GTCTTC GAAGAC(2/6)
BsaI 1204 GAGACC GGTCTC(1/5)
BsiHKAI 936 GTGCAC G[AT]GC[AT]^C
BsmFI 1168 GGGAC GGGAC(10/14)
BsmI 230 GCATTC GAATGC(1/-1)
BsoBI 146 CCCGAG C^[CT]CG[GA]G
BspHI 459 TCATGA T^CATGA
BstF5I 480 CATCC GGATG(2/0)
BstUI 879 CGCG CG^CG
Bsu36I 783 CCTGAGG CC^T[ACGT]AGG
Cfr13I 138 GGACC G^G[ACGT]CC
DraII 137 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 572 CCATGG C^C[GA][CT]GG
Eco81I 783 CCTGAGG CC^T[ACGT]AGG
EcoO109I 137 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 694 GATATC GAT^ATC
EcoT14I 572 CCATGG C^C[AT][AT]GG
FokI 480 CATCC GGATG(9/13)
HaeIII 927 GGCC GG^CC
HapII 874 CCGG C^CGG
HgaI 877 GACGC GACGC(5/10)
HindIII 1272 AAGCTT A^AGCTT
HinfI 1170 GACTC G^A[ACGT]TC
HpaII 874 CCGG C^CGG
MaeI 167 CTAG C^TAG
MaeII 585 ACGT A^CGT
MslI 759 CACATGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 950 CCGCTG C[AC]G^C[GT]G
MspI 874 CCGG C^CGG
MvnI 879 CGCG CG^CG
NciI 873 CCCGG CC^[GC]GG
NcoI 572 CCATGG C^CATGG
NspI 760 ACATGC [GA]CATG^[CT]
PleI 1170 GACTC GAGTC(4/5)
PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 584 AACGTT AA^CGTT
RcaI 459 TCATGA T^CATGA
Sau96I 138 GGACC G^G[ACGT]CC
SfcI 300 CTATAG C^T[GA][CT]AG
StyI 572 CCATGG C^C[AT][AT]GG
Tsp45I 466 GTGAC ^GT[GC]AC
XcmI 890 CCAGAGTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BamHI 274 GGATCC G^GATCC
390 GGATCC
BbvI 1405 GCAGC GCAGC(8/12)
886 GCTGC
BlpI 952 GCTGAGC GC^T[ACGT]AGC
1108 GCTGAGC
BmyI 895 GTGCCC G[AGT]GC[ACT]^C
936 GTGCAC
Bpu1102I 952 GCTGAGC GC^T[ACGT]AGC
1108 GCTGAGC
BseRI 484 CTCCTC GAGGAG(10/8)
786 GAGGAG
BsmAI 321 GAGAC GTCTC(1/5)
1204 GAGAC
Bsp1286I 895 GTGCCC G[AGT]GC[ACT]^C
936 GTGCAC
Bsp1407I 401 TGTACA T^GTACA
727 TGTACA
BsrGI 401 TGTACA T^GTACA
727 TGTACA
BsrI 600 ACTGG ACTGG(1/-1)
356 CCAGT
Cac8I 1003 GCTTGC GC[ACGT]^[ACGT]GC
1113 GCAAGC
CelII 952 GCTGAGC GC^T[ACGT]AGC
1108 GCTGAGC
HpaI 307 GTTAAC GTT^AAC
1330 GTTAAC
NlaIV 274 GGATCC GG[ACGT]^[ACGT]CC
390 GGATCC
SspBI 401 TGTACA T^GTACA
727 TGTACA
TseI 886 GCTGC G^C[AT]GC
1405 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BsiYI
BslI BspMI BssKI BstNI BstYI
DdeI DpnII EcoRII Fnu4HI HincII
HindII HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI SfaNI Tru9I Tsp509I TspRI
XhoII