Restriction map for
pFN21AE1438
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflIII AgeI AhdI AlwI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbsI BbvCI BciVI BfaI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DsaI
Eam1105I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 610 GACGTC GACGT^C
AcyI 610 GACGTC G[GA]^CG[CT]C
AflII 286 CTTAAG C^TTAAG
Alw44I 563 GTGCAC G^TGCAC
Aor51HI 293 AGCGCT AGC^GCT
ApaLI 563 GTGCAC G^TGCAC
BalI 690 TGGCCA TGG^CCA
BanI 678 GGTGCC G^G[CT][GA]CC
BbrPI 865 CACGTG CAC^GTG
BcgI 491 CGAGCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 525 TGATCA T^GATCA
BfrI 286 CTTAAG C^TTAAG
BsaAI 865 CACGTG [CT]AC^GT[GA]
BsaHI 610 GACGTC G[GA]^CG[CT]C
BsaWI 17 ACCGGA [AT]^CCGG[AT]
BsgI 673 GTGCAG GTGCAG(16/14)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsmAI 107 GAGAC GTCTC(1/5)
BsmFI 452 GGGAC GGGAC(10/14)
BspMI 675 GCAGGT ACCTGC(4/8)
BsrBI 24 CCGCTC CCGCTC(-3/-3)
BsrDI 486 CATTGC GCAATG(2/0)
BssHII 82 GCGCGC G^CGCGC
BssSI 310 CACGAG CACGAG(-5/-1)
BstEII 654 GGTGACC G^GT[ACGT]ACC
BstPI 654 GGTGACC G^GT[ACGT]ACC
BstXI 215 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 550 CTCAG C^T[ACGT]AG
DpnII 526 GATC ^GATC
DraII 509 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 649 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 325 GACAAGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 41 CGGCCG C^GGCCG
EarI 229 GAAGAG CTCTTC(1/4)
EclXI 41 CGGCCG C^GGCCG
Eco47III 293 AGCGCT AGC^GCT
Eco52I 41 CGGCCG C^GGCCG
EcoO109I 509 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 654 GGTGACC G^GT[ACGT]ACC
FbaI 525 TGATCA T^GATCA
HgaI 803 GCGTC GACGC(5/10)
Hin1I 610 GACGTC G[GA]^CG[CT]C
HphI 654 GGTGA GGTGA(8/7)
Ksp632I 229 GAAGAG CTCTTC(1/4)
MboI 526 GATC ^GATC
MluNI 690 TGGCCA TGG^CCA
MscI 690 TGGCCA TGG^CCA
MseI 287 TTAA T^TAA
NdeII 526 GATC ^GATC
PmaCI 865 CACGTG CAC^GTG
PmlI 865 CACGTG CAC^GTG
PvuII 94 CAGCTG CAG^CTG
Sau3AI 526 GATC ^GATC
Tru9I 287 TTAA T^TAA
XcmI 215 CCATTATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 95 AGCT AG^CT
764 AGCT
BanII 49 GGGCTC G[GA]GC[CT]^C
313 GAGCCC
BcnI 54 CCCGG CC^[GC]GG
682 CCGGG
BglI 161 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
692 GCCAGGATGGC
BsrFI 302 ACCGGC [GA]^CCGG[CT]
467 ACCGGC
Cfr10I 302 ACCGGC [GA]^CCGG[CT]
467 ACCGGC
EaeI 41 CGGCCG [CT]^GGCC[GA]
690 TGGCCA
EcoT14I 414 CCTTGG C^C[AT][AT]GG
605 CCAAGG
HaeII 293 AGCGCT [GA]GCGC^[CT]
474 GGCGCT
HinfI 211 GACTC G^A[ACGT]TC
357 GACTC
MslI 201 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
861 CATCCACGTG
MspA1I 94 CAGCTG C[AC]G^C[GT]G
175 CAGCGG
NciI 54 CCCGG CC^[GC]GG
682 CCGGG
PleI 211 GACTC GAGTC(4/5)
357 GACTC
StyI 414 CCTTGG C^C[AT][AT]GG
605 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AfaI AspHI AvaII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MaeIII MboII MnlI MspI
MvaI MvnI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI SmlI
TaqI TseI Tsp45I TspRI TthHB8I