Restriction map for
pFN21AE6246
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 179 AAATTT [GA]^AATT[CT]
AlwI 390 GATCC GGATC(4/5)
Aor51HI 704 AGCGCT AGC^GCT
ApoI 179 AAATTT [GA]^AATT[CT]
AspHI 199 GAGCTC G[AT]GC[AT]^C
AvaI 46 CCCGGG C^[CT]CG[GA]G
BalI 449 TGGCCA TGG^CCA
BanI 238 GGCACC G^G[CT][GA]CC
BsaWI 267 ACCGGA [AT]^CCGG[AT]
BseRI 433 GAGGAG GAGGAG(10/8)
BsgI 96 GTGCAG GTGCAG(16/14)
BsiHKAI 199 GAGCTC G[AT]GC[AT]^C
BsmFI 491 GGGAC GGGAC(10/14)
BsmI 73 GAATGC GAATGC(1/-1)
BsoBI 46 CCCGGG C^[CT]CG[GA]G
BsrI 594 ACTGG ACTGG(1/-1)
BstYI 389 AGATCC [GA]^GATC[CT]
DpnII 390 GATC ^GATC
DsaI 356 CCATGG C^C[GA][CT]GG
EagI 119 CGGCCG C^GGCCG
EclXI 119 CGGCCG C^GGCCG
Eco47III 704 AGCGCT AGC^GCT
Eco52I 119 CGGCCG C^GGCCG
EcoT14I 356 CCATGG C^C[AT][AT]GG
FauI 66 GCGGG CCCGC(4/6)
HaeII 704 AGCGCT [GA]GCGC^[CT]
HinfI 442 GACTC G^A[ACGT]TC
MaeII 94 ACGT A^CGT
MaeIII 746 GTTAC ^GT[ACGT]AC
MboI 390 GATC ^GATC
MflI 389 AGATCC [GA]^GATC[CT]
MluNI 449 TGGCCA TGG^CCA
MscI 449 TGGCCA TGG^CCA
MseI 577 TTAA T^TAA
MslI 514 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 208 CAGCTG C[AC]G^C[GT]G
NcoI 356 CCATGG C^CATGG
NdeII 390 GATC ^GATC
NheI 285 GCTAGC G^CTAGC
PleI 442 GACTC GAGTC(4/5)
PvuII 208 CAGCTG CAG^CTG
SacI 199 GAGCTC GAGCT^C
Sau3AI 390 GATC ^GATC
SfaNI 427 GATGC GCATC(5/9)
SmaI 46 CCCGGG CCC^GGG
SmlI 230 CTCAAG C^T[CT][GA]AG
StyI 356 CCATGG C^C[AT][AT]GG
Tru9I 577 TTAA T^TAA
TspRI 525 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 389 AGATCC [GA]^GATC[CT]
XmaCI 46 CCCGGG C^CCGGG
XmaI 46 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 5 CGCG CG^CG
90 CGCG
BanII 49 GGGCTC G[GA]GC[CT]^C
199 GAGCTC
BfaI 109 CTAG C^TAG
286 CTAG
BmyI 49 GGGCTC G[AGT]GC[ACT]^C
199 GAGCTC
BsiEI 59 CGACCG CG[GA][CT]^CG
119 CGGCCG
Bsp1286I 49 GGGCTC G[AGT]GC[ACT]^C
199 GAGCTC
BspMI 190 ACCTGC ACCTGC(4/8)
464 ACCTGC
BstUI 5 CGCG CG^CG
90 CGCG
BstXI 513 CCACCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
658 CCAGAGTGGTGG
Cfr13I 43 GGCCC G^G[ACGT]CC
68 GGGCC
DdeI 250 CTCAG C^T[ACGT]AG
459 CTCAG
EaeI 119 CGGCCG [CT]^GGCC[GA]
449 TGGCCA
EarI 370 GAAGAG CTCTTC(1/4)
644 GAAGAG
Ksp632I 370 GAAGAG CTCTTC(1/4)
644 GAAGAG
MaeI 109 CTAG C^TAG
286 CTAG
MvnI 5 CGCG CG^CG
90 CGCG
PstI 205 CTGCAG CTGCA^G
616 CTGCAG
Sau96I 43 GGCCC G^G[ACGT]CC
68 GGGCC
SfcI 205 CTGCAG C^T[GA][CT]AG
616 CTGCAG
Tsp509I 180 AATT ^AATT
454 AATT
XcmI 510 CCACCACCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
678 CCAGAAACGGTATGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BspHI BssKI BstF5I
BstNI Cac8I CfoI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
RcaI ScrFI TseI