Restriction map for
pFN21AB9901
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau3AI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AatII 259 GACGTC GACGT^C
AcyI 259 GACGTC G[GA]^CG[CT]C
ApaI 600 GGGCCC GGGCC^C
AspHI 568 GAGCTC G[AT]GC[AT]^C
AspI 304 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 283 GAAGAC GAAGAC(2/6)
BpuAI 283 GAAGAC GAAGAC(2/6)
BsaHI 259 GACGTC G[GA]^CG[CT]C
BsaI 309 GGTCTC GGTCTC(1/5)
BsgI 519 CTGCAC GTGCAG(16/14)
BsiHKAI 568 GAGCTC G[AT]GC[AT]^C
BsmBI 194 CGTCTC CGTCTC(1/5)
BsmFI 682 GGGAC GGGAC(10/14)
BsrI 68 ACTGG ACTGG(1/-1)
BstAPI 78 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 253 GACTATGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 293 TGGCCG [CT]^GGCC[GA]
EarI 102 GAAGAG CTCTTC(1/4)
EcoNI 492 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 379 GGCGCT [GA]GCGC^[CT]
Hin1I 259 GACGTC G[GA]^CG[CT]C
Ksp632I 102 GAAGAG CTCTTC(1/4)
NspI 216 ACATGC [GA]CATG^[CT]
PflFI 304 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 568 GAGCTC GAGCT^C
Tsp509I 693 AATT ^AATT
Tth111I 304 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 296 CCGC CCGC(-3/-1)
377 GCGG
AfaI 474 GTAC GT^AC
582 GTAC
BanII 568 GAGCTC G[GA]GC[CT]^C
600 GGGCCC
BbvCI 180 CCTCAGC CCTCAGC(-5/-2)
543 CCTCAGC
BfaI 52 CTAG C^TAG
706 CTAG
BmyI 568 GAGCTC G[AGT]GC[ACT]^C
600 GGGCCC
BsmAI 195 GTCTC GTCTC(1/5)
310 GTCTC
Bsp1286I 568 GAGCTC G[AGT]GC[ACT]^C
600 GGGCCC
BstF5I 525 CATCC GGATG(2/0)
363 GGATG
CfoI 342 GCGC GCG^C
380 GCGC
FokI 525 CATCC GGATG(9/13)
363 GGATG
HhaI 342 GCGC GCG^C
380 GCGC
HinP1I 342 GCGC G^CGC
380 GCGC
HinfI 575 GATTC G^A[ACGT]TC
736 GATTC
MaeI 52 CTAG C^TAG
706 CTAG
MslI 217 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
742 CACAAATGTG
PflMI 22 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCAGGACCTGG
PstI 484 CTGCAG CTGCA^G
649 CTGCAG
RsaI 474 GTAC GT^AC
582 GTAC
SexAI 287 ACCTGGT A^CC[AT]GGT
305 ACCTGGT
TaqI 10 TCGA T^CGA
590 TCGA
TfiI 575 GATTC G^A[AT]TC
736 GATTC
TthHB8I 10 TCGA T^CGA
590 TCGA
Van91I 22 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCAGGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwNI AvaII BbvI BcnI
BpmI BsaJI BsiYI BslI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MaeII MaeIII MboII
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV PpuMI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I TspRI