Restriction map for
pFN21AE4488
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FokI FseI FspI HaeII HpaI
KasI KpnI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1428 GTCTAC GT^[AC][GT]AC
AcyI 1282 GGCGTC G[GA]^CG[CT]C
AfaI 141 GTAC GT^AC
BamHI 7 GGATCC G^GATCC
BciVI 1167 GGATAC GTATCC(6/5)
BclI 644 TGATCA T^GATCA
BglI 263 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1282 GGCGTC G[GA]^CG[CT]C
BspMI 1325 ACCTGC ACCTGC(4/8)
BsrFI 1046 ACCGGC [GA]^CCGG[CT]
BstAPI 165 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 7 GGATCC [GA]^GATC[CT]
Cac8I 951 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 53 GCGC GCG^C
Cfr10I 1046 ACCGGC [GA]^CCGG[CT]
DraI 255 TTTAAA TTT^AAA
DraIII 166 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 110 CCGCGG C^C[GA][CT]GG
EaeI 40 CGGCCG [CT]^GGCC[GA]
EagI 40 CGGCCG C^GGCCG
EclXI 40 CGGCCG C^GGCCG
Eco52I 40 CGGCCG C^GGCCG
EcoNI 715 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 916 GAATTC G^AATTC
EcoT14I 942 CCAAGG C^C[AT][AT]GG
FbaI 644 TGATCA T^GATCA
HgaI 1283 GCGTC GACGC(5/10)
HhaI 53 GCGC GCG^C
Hin1I 1282 GGCGTC G[GA]^CG[CT]C
HinP1I 53 GCGC G^CGC
HincII 798 GTTGAC GT[CT]^[GA]AC
HindII 798 GTTGAC GT[CT]^[GA]AC
HindIII 554 AAGCTT A^AGCTT
HphI 321 GGTGA GGTGA(8/7)
KspI 110 CCGCGG CCGC^GG
MflI 7 GGATCC [GA]^GATC[CT]
MslI 532 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
RsaI 141 GTAC GT^AC
SacII 110 CCGCGG CCGC^GG
SexAI 167 ACCTGGT A^CC[AT]GGT
SfcI 491 CTATAG C^T[GA][CT]AG
SmlI 1227 CTTGAG C^T[CT][GA]AG
StyI 942 CCAAGG C^C[AT][AT]GG
XhoII 7 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 200 GGGCCC GGGCC^C
402 GGGCCC
AspHI 880 GAGCAC G[AT]GC[AT]^C
1321 GAGCAC
AvaII 185 GGACC G^G[AT]CC
1434 GGTCC
BanII 200 GGGCCC G[GA]GC[CT]^C
402 GGGCCC
BcnI 27 CCGGG CC^[GC]GG
84 CCGGG
BfaI 367 CTAG C^TAG
548 CTAG
BpmI 749 CTGGAG CTGGAG(16/14)
1277 CTGGAG
BseRI 101 GAGGAG GAGGAG(10/8)
1118 GAGGAG
BsiHKAI 880 GAGCAC G[AT]GC[AT]^C
1321 GAGCAC
DraII 401 AGGGCCC [GA]G^G[ACGT]CC[CT]
402 GGGCCCT
DrdI 106 GACCCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACCATGATGTC
EarI 1089 CTCTTC CTCTTC(1/4)
341 GAAGAG
EcoO109I 401 AGGGCCC [GA]G^G[ACGT]CC[CT]
402 GGGCCCT
Ksp632I 1089 CTCTTC CTCTTC(1/4)
341 GAAGAG
MaeI 367 CTAG C^TAG
548 CTAG
MseI 256 TTAA T^TAA
1096 TTAA
NciI 27 CCGGG CC^[GC]GG
84 CCGGG
SspI 444 AATATT AAT^ATT
963 AATATT
Tru9I 256 TTAA T^TAA
1096 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BbvI BmyI BsaI BsaJI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstNI BstUI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I