Restriction map for
pFN21AE1549
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
ClaI CpoI DraII DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FseI FspI HaeII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MluI MroI MslI
MvnI NarI NciI NlaIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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BbsI 385 GAAGAC GAAGAC(2/6)
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
BciVI 1447 GGATAC GTATCC(6/5)
BclI 1151 TGATCA T^GATCA
BpmI 1205 CTCCAG CTGGAG(16/14)
BpuAI 385 GAAGAC GAAGAC(2/6)
BseRI 1175 CTCCTC GAGGAG(10/8)
BsgI 359 CTGCAC GTGCAG(16/14)
BsmFI 776 GGGAC GGGAC(10/14)
BsmI 393 GAATGC GAATGC(1/-1)
BspHI 1154 TCATGA T^CATGA
BsrFI 160 GCCGGC [GA]^CCGG[CT]
Bst1107I 1194 GTATAC GTA^TAC
BstYI 1092 GGATCT [GA]^GATC[CT]
BstZ17I 1194 GTATAC GTA^TAC
CfoI 119 GCGC GCG^C
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
DraI 520 TTTAAA TTT^AAA
DrdI 1398 GACAATTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 314 CCATGG C^C[GA][CT]GG
EcoT22I 278 ATGCAT ATGCA^T
FbaI 1151 TGATCA T^GATCA
HapII 161 CCGG C^CGG
HhaI 119 GCGC GCG^C
HinP1I 119 GCGC G^CGC
HincII 668 GTCAAC GT[CT]^[GA]AC
HindII 668 GTCAAC GT[CT]^[GA]AC
HpaII 161 CCGG C^CGG
MfeI 260 CAATTG C^AATTG
MflI 1092 GGATCT [GA]^GATC[CT]
MspI 161 CCGG C^CGG
MunI 260 CAATTG C^AATTG
NaeI 160 GCCGGC GCC^GGC
NcoI 314 CCATGG C^CATGG
NdeI 281 CATATG CA^TATG
NgoMIV 160 GCCGGC G^CCGGC
NheI 634 GCTAGC G^CTAGC
NsiI 278 ATGCAT ATGCA^T
PleI 1255 GACTC GAGTC(4/5)
PstI 37 CTGCAG CTGCA^G
RcaI 1154 TCATGA T^CATGA
SpeI 973 ACTAGT A^CTAGT
SphI 234 GCATGC GCATG^C
Tsp45I 79 GTGAC ^GT[GC]AC
XbaI 270 TCTAGA T^CTAGA
XcmI 639 CCATTGTTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1092 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 896 GGCGTC G[GA]^CG[CT]C
1352 GGCGTC
AlwI 147 GATCC GGATC(4/5)
1092 GGATC
AspHI 616 GAGCTC G[AT]GC[AT]^C
782 GAGCTC
BalI 998 TGGCCA TGG^CCA
1166 TGGCCA
BanII 616 GAGCTC G[GA]GC[CT]^C
782 GAGCTC
BmyI 616 GAGCTC G[AGT]GC[ACT]^C
782 GAGCTC
BsaHI 896 GGCGTC G[GA]^CG[CT]C
1352 GGCGTC
BsaJI 228 CCTTGG C^C[ACGT][ACGT]GG
314 CCATGG
BsiHKAI 616 GAGCTC G[AT]GC[AT]^C
782 GAGCTC
BsmAI 1004 GAGAC GTCTC(1/5)
837 GTCTC
Bsp1286I 616 GAGCTC G[AGT]GC[ACT]^C
782 GAGCTC
BsrI 466 CCAGT ACTGG(1/-1)
1498 CCAGT
EcoT14I 228 CCTTGG C^C[AT][AT]GG
314 CCATGG
FauI 181 CCCGC CCCGC(4/6)
43 GCGGG
Hin1I 896 GGCGTC G[GA]^CG[CT]C
1352 GGCGTC
MaeIII 79 GTGAC ^GT[ACGT]AC
1508 GTTAC
MluNI 998 TGGCCA TGG^CCA
1166 TGGCCA
MscI 998 TGGCCA TGG^CCA
1166 TGGCCA
MspA1I 175 CAGCTG C[AC]G^C[GT]G
691 CAGCTG
NspI 234 GCATGC [GA]CATG^[CT]
528 GCATGT
PflMI 63 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
223 CCATTCCTTGG
PvuII 175 CAGCTG CAG^CTG
691 CAGCTG
SacI 616 GAGCTC GAGCT^C
782 GAGCTC
SexAI 167 ACCTGGT A^CC[AT]GGT
741 ACCAGGT
SfcI 37 CTGCAG C^T[GA][CT]AG
408 CTACAG
SmlI 839 CTCAAG C^T[CT][GA]AG
1087 CTCAAG
StyI 228 CCTTGG C^C[AT][AT]GG
314 CCATGG
TfiI 1059 GAATC G^A[AT]TC
1479 GATTC
Van91I 63 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
223 CCATTCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccI AciI AcsI AluI ApoI
AvaII BbvI BfaI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HgaI HinfI HphI
ItaI MaeI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I TspRI