Restriction map for
pFN21AE6219
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstYI BstZ17I CelII Cfr10I CpoI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AfaI 237 GTAC GT^AC
AlwNI 46 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 408 CCTGAGG CC^T[ACGT]AGG
AvaI 162 CCCGGG C^[CT]CG[GA]G
AviII 344 TGCGCA TGC^GCA
BbsI 261 GAAGAC GAAGAC(2/6)
BglI 28 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 261 GAAGAC GAAGAC(2/6)
BsiEI 353 CGGCCG CG[GA][CT]^CG
BsmAI 467 GAGAC GTCTC(1/5)
BsmBI 467 GAGACG CGTCTC(1/5)
BsmFI 458 GGGAC GGGAC(10/14)
BsoBI 162 CCCGGG C^[CT]CG[GA]G
BspDI 214 ATCGAT AT^CGAT
BsrBI 454 GAGCGG CCGCTC(-3/-3)
BsrI 479 ACTGG ACTGG(1/-1)
BstNI 132 CCTGG CC^[AT]GG
BstXI 80 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 408 CCTGAGG CC^T[ACGT]AGG
CfoI 345 GCGC GCG^C
ClaI 214 ATCGAT AT^CGAT
DdeI 409 CTGAG C^T[ACGT]AG
DraIII 391 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 80 CCACGG C^C[GA][CT]GG
EagI 353 CGGCCG C^GGCCG
EclXI 353 CGGCCG C^GGCCG
Eco52I 353 CGGCCG C^GGCCG
Eco81I 408 CCTGAGG CC^T[ACGT]AGG
EcoNI 420 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 132 CCTGG ^CC[AT]GG
FspI 344 TGCGCA TGC^GCA
HhaI 345 GCGC GCG^C
HinP1I 345 GCGC G^CGC
MaeII 470 ACGT A^CGT
MaeIII 476 GTGAC ^GT[ACGT]AC
MslI 243 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 132 CCTGG CC^[AT]GG
NspI 347 GCATGT [GA]CATG^[CT]
PpuMI 458 GGGACCT [GA]G^G[AT]CC[CT]
PstI 67 CTGCAG CTGCA^G
PvuII 199 CAGCTG CAG^CTG
RsaI 237 GTAC GT^AC
SacI 187 GAGCTC GAGCT^C
SapI 54 GAAGAGC GCTCTTC(1/4)
SfcI 67 CTGCAG C^T[GA][CT]AG
SfiI 27 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 162 CCCGGG CCC^GGG
SmlI 367 CTCAAG C^T[CT][GA]AG
Tsp45I 476 GTGAC ^GT[GC]AC
XcmI 266 CCAGCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 162 CCCGGG C^CCGGG
XmaI 162 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 139 GAGCAC G[AT]GC[AT]^C
187 GAGCTC
AvaII 405 GGACC G^G[AT]CC
459 GGACC
BcnI 162 CCCGG CC^[GC]GG
163 CCGGG
BsaJI 80 CCACGG C^C[ACGT][ACGT]GG
162 CCCGGG
BsiHKAI 139 GAGCAC G[AT]GC[AT]^C
187 GAGCTC
BspMI 461 ACCTGC ACCTGC(4/8)
426 GCAGGT
DpnII 213 GATC ^GATC
304 GATC
DraII 371 AGGCCCT [GA]G^G[ACGT]CC[CT]
458 GGGACCT
EaeI 77 CGGCCA [CT]^GGCC[GA]
353 CGGCCG
EarI 54 GAAGAG CTCTTC(1/4)
226 GAAGAG
EcoO109I 371 AGGCCCT [GA]G^G[ACGT]CC[CT]
458 GGGACCT
HapII 163 CCGG C^CGG
519 CCGG
HinfI 535 GAGTC G^A[ACGT]TC
541 GAGTC
HpaII 163 CCGG C^CGG
519 CCGG
HphI 249 GGTGA GGTGA(8/7)
475 GGTGA
Ksp632I 54 GAAGAG CTCTTC(1/4)
226 GAAGAG
MboI 213 GATC ^GATC
304 GATC
MspA1I 151 CCGCTG C[AC]G^C[GT]G
199 CAGCTG
MspI 163 CCGG C^CGG
519 CCGG
NciI 162 CCCGG CC^[GC]GG
163 CCGGG
NdeII 213 GATC ^GATC
304 GATC
PleI 535 GAGTC GAGTC(4/5)
541 GAGTC
Sau3AI 213 GATC ^GATC
304 GATC
TspRI 142 CACTG CA[GC]TG[ACGT][ACGT]^
332 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BseRI BsgI BsiYI BslI
Bsp1286I BssKI Cac8I Cfr13I FauI
Fnu4HI HaeIII ItaI MboII MnlI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI TaqI TseI TthHB8I