Restriction map for
pFN21AE1412
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeI MamI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I TspRI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 34 GAATTC [GA]^AATT[CT]
AflIII 321 ACACGT A^C[GA][CT]GT
ApoI 34 GAATTC [GA]^AATT[CT]
AvaI 199 CCCGAG C^[CT]CG[GA]G
AvaII 113 GGTCC G^G[AT]CC
BalI 218 TGGCCA TGG^CCA
BanI 184 GGCACC G^G[CT][GA]CC
BcgI 290 GCAACGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 99 GGGCAC G[AGT]GC[ACT]^C
BsiEI 168 CGACCG CG[GA][CT]^CG
BsmAI 398 GAGAC GTCTC(1/5)
BsmBI 398 GAGACG CGTCTC(1/5)
BsoBI 199 CCCGAG C^[CT]CG[GA]G
Bsp1286I 99 GGGCAC G[AGT]GC[ACT]^C
BspHI 326 TCATGA T^CATGA
BspMI 24 GCAGGT ACCTGC(4/8)
BstF5I 66 GGATG GGATG(2/0)
BstYI 254 AGATCC [GA]^GATC[CT]
Cac8I 92 GCGAGC GC[ACGT]^[ACGT]GC
CfoI 305 GCGC GCG^C
EcoRI 34 GAATTC G^AATTC
FokI 66 GGATG GGATG(9/13)
HaeII 304 AGCGCC [GA]GCGC^[CT]
HhaI 305 GCGC GCG^C
HinP1I 305 GCGC G^CGC
KspI 383 CCGCGG CCGC^GG
MaeII 323 ACGT A^CGT
MboII 225 GAAGA GAAGA(8/7)
MflI 254 AGATCC [GA]^GATC[CT]
MluNI 218 TGGCCA TGG^CCA
MscI 218 TGGCCA TGG^CCA
MslI 102 CACGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PvuII 10 CAGCTG CAG^CTG
RcaI 326 TCATGA T^CATGA
SacII 383 CCGCGG CCGC^GG
SfaNI 80 GCATC GCATC(5/9)
Tsp509I 35 AATT ^AATT
XcmI 86 CCACCCGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 254 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 255 GATCC GGATC(4/5)
375 GATCC
DdeI 17 CTGAG C^T[ACGT]AG
349 CTGAG
EaeI 2 TGGCCG [CT]^GGCC[GA]
218 TGGCCA
FauI 89 CCCGC CCCGC(4/6)
147 GCGGG
HphI 408 GGTGA GGTGA(8/7)
83 TCACC
MaeIII 28 GTCAC ^GT[ACGT]AC
353 GTGAC
MspA1I 10 CAGCTG C[AC]G^C[GT]G
383 CCGCGG
MwoI 92 GCGAGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
296 GCTTCGTCAGC
NlaIV 113 GGTCCC GG[ACGT]^[ACGT]CC
184 GGCACC
StuI 44 AGGCCT AGG^CCT
263 AGGCCT
TaqI 167 TCGA T^CGA
278 TCGA
Tsp45I 28 GTCAC ^GT[GC]AC
353 GTGAC
TthHB8I 167 TCGA T^CGA
278 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BseRI BsiYI BslI BsmFI
BssKI BstNI BstUI Cfr13I DpnII
DsaI EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MboI MnlI MspI
MvaI MvnI NciI NdeII NlaIII
Sau3AI Sau96I ScrFI TseI