Restriction map for
pFN21AE0499
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BalI BamHI
BbeI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmeI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 719 GGTACC G^GTACC
Alw44I 269 GTGCAC G^TGCAC
AocI 1400 CCTTAGG CC^T[ACGT]AGG
ApaLI 269 GTGCAC G^TGCAC
AseI 1360 ATTAAT AT^TAAT
AsnI 1360 ATTAAT AT^TAAT
Asp718I 719 GGTACC G^GTACC
AspHI 269 GTGCAC G[AT]GC[AT]^C
AvaI 947 CTCGAG C^[CT]CG[GA]G
AvaII 367 GGACC G^G[AT]CC
BanI 719 GGTACC G^G[CT][GA]CC
BanII 470 GAGCCC G[GA]GC[CT]^C
BbrPI 266 CACGTG CAC^GTG
BbsI 297 GTCTTC GAAGAC(2/6)
BbvCI 170 CCTCAGC CCTCAGC(-5/-2)
BcgI 1887 CGATTCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 518 TGATCA T^GATCA
BglII 642 AGATCT A^GATCT
BpuAI 297 GTCTTC GAAGAC(2/6)
BsaI 2022 GAGACC GGTCTC(1/5)
BsaJI 473 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 1186 TCCGGT [AT]^CCGG[AT]
BsiHKAI 269 GTGCAC G[AT]GC[AT]^C
BsoBI 947 CTCGAG C^[CT]CG[GA]G
BstAPI 1169 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1400 CCTTAGG CC^T[ACGT]AGG
DraI 12 TTTAAA TTT^AAA
DraII 1847 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 2019 GAAGAG CTCTTC(1/4)
Eco81I 1400 CCTTAGG CC^T[ACGT]AGG
EcoO109I 1847 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 935 ATGCAT ATGCA^T
FauI 1950 GCGGG CCCGC(4/6)
FbaI 518 TGATCA T^GATCA
HapII 1187 CCGG C^CGG
HpaII 1187 CCGG C^CGG
KpnI 719 GGTACC GGTAC^C
Ksp632I 2019 GAAGAG CTCTTC(1/4)
MslI 1446 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1187 CCGG C^CGG
NdeI 1892 CATATG CA^TATG
NsiI 935 ATGCAT ATGCA^T
NspI 841 ACATGC [GA]CATG^[CT]
PaeR7I 947 CTCGAG C^TCGAG
PleI 763 GACTC GAGTC(4/5)
PmaCI 266 CACGTG CAC^GTG
PmlI 266 CACGTG CAC^GTG
PshBI 1360 ATTAAT AT^TAAT
SpeI 807 ACTAGT A^CTAGT
Tsp45I 893 GTGAC ^GT[GC]AC
XbaI 1077 TCTAGA T^CTAGA
XhoI 947 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AciI 1327 GCGG CCGC(-3/-1)
1950 GCGG
AlwI 568 GATCC GGATC(4/5)
1585 GATCC
BsaAI 266 CACGTG [CT]AC^GT[GA]
377 CACGTA
BseRI 1307 GAGGAG GAGGAG(10/8)
1987 GAGGAG
BsgI 305 CTGCAC GTGCAG(16/14)
1973 GTGCAG
BspMI 1489 ACCTGC ACCTGC(4/8)
1095 GCAGGT
Cac8I 1999 GCCAGC GC[ACGT]^[ACGT]GC
2003 GCAAGC
HaeIII 275 GGCC GG^CC
1848 GGCC
SfcI 209 CTGTAG C^T[GA][CT]AG
1580 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbvI BfaI BmyI BpmI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI