Restriction map for
pFN21AB0461
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BciVI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmBI BspDI BspEI BspMI
BsrBI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
HinfI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI TfiI
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 46 CGCG CG^CG
AfaI 717 GTAC GT^AC
AflIII 563 ACATGT A^C[GA][CT]GT
AocI 579 CCTGAGG CC^T[ACGT]AGG
AspHI 658 GAGCTC G[AT]GC[AT]^C
AspI 1048 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 468 GGACC G^G[AT]CC
BamHI 420 GGATCC G^GATCC
BanI 447 GGTGCC G^G[CT][GA]CC
BanII 658 GAGCTC G[GA]GC[CT]^C
BcgI 704 GCAACACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 671 TGATCA T^GATCA
BfaI 115 CTAG C^TAG
BglII 1241 AGATCT A^GATCT
BmyI 658 GAGCTC G[AGT]GC[ACT]^C
BsaI 459 GAGACC GGTCTC(1/5)
BsiEI 62 CGACCG CG[GA][CT]^CG
BsiHKAI 658 GAGCTC G[AT]GC[AT]^C
BsmI 303 GAATGC GAATGC(1/-1)
Bsp1286I 658 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 716 TGTACA T^GTACA
BspLU11I 563 ACATGT A^CATGT
BsrDI 700 CATTGC GCAATG(2/0)
BsrFI 410 GCCGGC [GA]^CCGG[CT]
BsrGI 716 TGTACA T^GTACA
BstUI 46 CGCG CG^CG
Bsu36I 579 CCTGAGG CC^T[ACGT]AGG
CfoI 45 GCGC GCG^C
Cfr10I 410 GCCGGC [GA]^CCGG[CT]
DsaI 464 CCATGG C^C[GA][CT]GG
Eco81I 579 CCTGAGG CC^T[ACGT]AGG
EcoRI 91 GAATTC G^AATTC
EcoT14I 464 CCATGG C^C[AT][AT]GG
FbaI 671 TGATCA T^GATCA
HhaI 45 GCGC GCG^C
HinP1I 45 GCGC G^CGC
HpaI 1324 GTTAAC GTT^AAC
MaeI 115 CTAG C^TAG
MspA1I 569 CCGCTG C[AC]G^C[GT]G
MvnI 46 CGCG CG^CG
NaeI 410 GCCGGC GCC^GGC
NcoI 464 CCATGG C^CATGG
NgoMIV 410 GCCGGC G^CCGGC
PflFI 1048 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 717 GTAC GT^AC
SacI 658 GAGCTC GAGCT^C
SalI 1216 GTCGAC G^TCGAC
SapI 796 GAAGAGC GCTCTTC(1/4)
SexAI 956 ACCAGGT A^CC[AT]GGT
SmaI 1151 CCCGGG CCC^GGG
SmlI 1123 CTTGAG C^T[CT][GA]AG
SspBI 716 TGTACA T^GTACA
StyI 464 CCATGG C^C[AT][AT]GG
Tth111I 1048 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 464 CCATGGACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1151 CCCGGG C^CCGGG
XmaI 1151 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AccI 324 GTCTAC GT^[AC][GT]AC
1216 GTCGAC
AvaI 232 CCCGAG C^[CT]CG[GA]G
1151 CCCGGG
BsmAI 459 GAGAC GTCTC(1/5)
949 GAGAC
BsoBI 232 CCCGAG C^[CT]CG[GA]G
1151 CCCGGG
Cfr13I 408 GGGCC G^G[ACGT]CC
468 GGACC
DdeI 457 CTGAG C^T[ACGT]AG
580 CTGAG
HphI 193 GGTGA GGTGA(8/7)
669 GGTGA
NspI 563 ACATGT [GA]CATG^[CT]
752 ACATGC
Sau96I 408 GGGCC G^G[ACGT]CC
468 GGACC
SfaNI 760 GCATC GCATC(5/9)
1260 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BcnI BsaJI BseRI BsiYI
BslI BsmFI BspHI BssKI BstF5I
BstNI BstYI Cac8I DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HincII HindII HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RcaI Sau3AI ScrFI SspI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII