Restriction map for
pFN21AE6325
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 494 TCCGGA T^CCGGA
AlwI 621 GGATC GGATC(4/5)
AlwNI 763 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 424 GAGCTC G[AT]GC[AT]^C
BalI 1079 TGGCCA TGG^CCA
BanI 1121 GGTGCC G^G[CT][GA]CC
BanII 424 GAGCTC G[GA]GC[CT]^C
BbsI 447 GAAGAC GAAGAC(2/6)
BclI 566 TGATCA T^GATCA
BglII 46 AGATCT A^GATCT
BlpI 426 GCTCAGC GC^T[ACGT]AGC
BmyI 424 GAGCTC G[AGT]GC[ACT]^C
BpmI 769 CTGGAG CTGGAG(16/14)
Bpu1102I 426 GCTCAGC GC^T[ACGT]AGC
BpuAI 447 GAAGAC GAAGAC(2/6)
BsaWI 494 TCCGGA [AT]^CCGG[AT]
BseAI 494 TCCGGA T^CCGGA
BsiHKAI 424 GAGCTC G[AT]GC[AT]^C
BsmAI 550 GAGAC GTCTC(1/5)
Bsp1286I 424 GAGCTC G[AGT]GC[ACT]^C
BspEI 494 TCCGGA T^CCGGA
BspHI 531 TCATGA T^CATGA
CelII 426 GCTCAGC GC^T[ACGT]AGC
DraII 773 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1079 TGGCCA [CT]^GGCC[GA]
EcoNI 1077 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 773 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1087 GAATTC G^AATTC
EcoT14I 1082 CCAAGG C^C[AT][AT]GG
EcoT22I 698 ATGCAT ATGCA^T
FbaI 566 TGATCA T^GATCA
HaeIII 1080 GGCC GG^CC
HapII 495 CCGG C^CGG
HincII 1299 GTCAAC GT[CT]^[GA]AC
HindII 1299 GTCAAC GT[CT]^[GA]AC
HpaII 495 CCGG C^CGG
MaeII 187 ACGT A^CGT
MaeIII 1037 GTGAC ^GT[ACGT]AC
MluNI 1079 TGGCCA TGG^CCA
MroI 494 TCCGGA T^CCGGA
MscI 1079 TGGCCA TGG^CCA
MslI 1003 CATTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 495 CCGG C^CGG
MwoI 321 GCCCAATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 698 ATGCAT ATGCA^T
PflMI 762 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 531 TCATGA T^CATGA
SacI 424 GAGCTC GAGCT^C
SmlI 169 CTTGAG C^T[CT][GA]AG
SspI 821 AATATT AAT^ATT
StyI 1082 CCAAGG C^C[AT][AT]GG
Tsp45I 1037 GTGAC ^GT[GC]AC
Van91I 762 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 738 TCTAGA T^CTAGA
XcmI 364 CCAGTTCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 378 GCGG CCGC(-3/-1)
904 GCGG
AcsI 992 AAATTC [GA]^AATT[CT]
1087 GAATTC
ApoI 992 AAATTC [GA]^AATT[CT]
1087 GAATTC
Asp700I 259 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
486 GAAAACTTTC
AvaII 579 GGACC G^G[AT]CC
774 GGACC
BbvI 318 GCAGC GCAGC(8/12)
916 GCTGC
BfaI 281 CTAG C^TAG
739 CTAG
BsaJI 575 CCCAGG C^C[ACGT][ACGT]GG
1082 CCAAGG
BsrDI 1043 CATTGC GCAATG(2/0)
1169 GCAATG
BstYI 46 AGATCT [GA]^GATC[CT]
621 GGATCT
Cfr13I 579 GGACC G^G[ACGT]CC
774 GGACC
EarI 73 GAAGAG CTCTTC(1/4)
106 GAAGAG
Fnu4HI 318 GCAGC GC^[ACGT]GC
916 GCTGC
HindIII 112 AAGCTT A^AGCTT
292 AAGCTT
ItaI 318 GCAGC GC^[ACGT]GC
916 GCTGC
Ksp632I 73 GAAGAG CTCTTC(1/4)
106 GAAGAG
MaeI 281 CTAG C^TAG
739 CTAG
MflI 46 AGATCT [GA]^GATC[CT]
621 GGATCT
MspA1I 383 CAGCTG C[AC]G^C[GT]G
802 CAGCTG
PvuII 383 CAGCTG CAG^CTG
802 CAGCTG
Sau96I 579 GGACC G^G[ACGT]CC
774 GGACC
TaqI 537 TCGA T^CGA
663 TCGA
TseI 318 GCAGC G^C[AT]GC
916 GCTGC
TthHB8I 537 TCGA T^CGA
663 TCGA
XhoII 46 AGATCT [GA]^GATC[CT]
621 GGATCT
XmnI 259 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
486 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BsiYI BslI BsrI BssKI
BstF5I BstNI DdeI DpnII EcoRII
FokI HinfI MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI SfaNI TfiI
Tru9I Tsp509I TspRI