Restriction map for
pFN21AE3591
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BanI BbeI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PflFI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 779 CGCG CG^CG
AcsI 788 AAATTT [GA]^AATT[CT]
AflIII 361 ACGTGT A^C[GA][CT]GT
Alw44I 104 GTGCAC G^TGCAC
ApaLI 104 GTGCAC G^TGCAC
ApoI 788 AAATTT [GA]^AATT[CT]
Asp700I 65 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 104 GTGCAC G[AT]GC[AT]^C
AvaI 1111 CTCGAG C^[CT]CG[GA]G
BalI 1014 TGGCCA TGG^CCA
BamHI 986 GGATCC G^GATCC
BanII 1163 GGGCTC G[GA]GC[CT]^C
BbrPI 360 CACGTG CAC^GTG
BbsI 288 GAAGAC GAAGAC(2/6)
BclI 498 TGATCA T^GATCA
BfaI 1084 CTAG C^TAG
BglII 165 AGATCT A^GATCT
BpuAI 288 GAAGAC GAAGAC(2/6)
BseRI 1054 CTCCTC GAGGAG(10/8)
BsiHKAI 104 GTGCAC G[AT]GC[AT]^C
BsmFI 957 GTCCC GGGAC(10/14)
BsmI 883 GAATGC GAATGC(1/-1)
BsoBI 1111 CTCGAG C^[CT]CG[GA]G
BsrFI 701 ACCGGC [GA]^CCGG[CT]
BstEII 1134 GGTTACC G^GT[ACGT]ACC
BstPI 1134 GGTTACC G^GT[ACGT]ACC
BstUI 779 CGCG CG^CG
CfoI 1107 GCGC GCG^C
Cfr10I 701 ACCGGC [GA]^CCGG[CT]
Cfr13I 1071 GGCCC G^G[ACGT]CC
EaeI 1014 TGGCCA [CT]^GGCC[GA]
EcoO65I 1134 GGTTACC G^GT[ACGT]ACC
EcoT14I 116 CCAAGG C^C[AT][AT]GG
EcoT22I 885 ATGCAT ATGCA^T
FbaI 498 TGATCA T^GATCA
HaeII 1106 AGCGCC [GA]GCGC^[CT]
HapII 702 CCGG C^CGG
HgaI 823 GACGC GACGC(5/10)
HhaI 1107 GCGC GCG^C
HinP1I 1107 GCGC G^CGC
HpaII 702 CCGG C^CGG
MaeI 1084 CTAG C^TAG
MluNI 1014 TGGCCA TGG^CCA
MscI 1014 TGGCCA TGG^CCA
MslI 1062 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 702 CCGG C^CGG
MvnI 779 CGCG CG^CG
NlaIV 986 GGATCC GG[ACGT]^[ACGT]CC
NsiI 885 ATGCAT ATGCA^T
PaeR7I 1111 CTCGAG C^TCGAG
PmaCI 360 CACGTG CAC^GTG
PmlI 360 CACGTG CAC^GTG
Sau96I 1071 GGCCC G^G[ACGT]CC
SfcI 706 CTACAG C^T[GA][CT]AG
SmlI 1111 CTCGAG C^T[CT][GA]AG
SspI 66 AATATT AAT^ATT
StyI 116 CCAAGG C^C[AT][AT]GG
XhoI 1111 CTCGAG C^TCGAG
XmnI 65 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AocI 237 CCTGAGG CC^T[ACGT]AGG
564 CCTGAGG
BlpI 246 GCTCAGC GC^T[ACGT]AGC
1165 GCTCAGC
BmyI 104 GTGCAC G[AGT]GC[ACT]^C
1163 GGGCTC
Bpu1102I 246 GCTCAGC GC^T[ACGT]AGC
1165 GCTCAGC
BsaAI 360 CACGTG [CT]AC^GT[GA]
656 CACGTA
Bsp1286I 104 GTGCAC G[AGT]GC[ACT]^C
1163 GGGCTC
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
564 CCTGAGG
CelII 246 GCTCAGC GC^T[ACGT]AGC
1165 GCTCAGC
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
564 CCTGAGG
FauI 1029 GCGGG CCCGC(4/6)
1161 GCGGG
HphI 699 TCACC GGTGA(8/7)
1208 TCACC
MaeII 361 ACGT A^CGT
657 ACGT
PflMI 116 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCACGAATTGG
Tsp45I 698 GTCAC ^GT[GC]AC
1207 GTCAC
Van91I 116 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCACGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BpmI BsaJI BsgI BsiYI
BslI BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
StuI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII