Restriction map for
pFN21AE3919
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI AocI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BclI BfaI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI HgaI Hin1I
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MluI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 639 GTCTAC GT^[AC][GT]AC
AflII 1903 CTTAAG C^TTAAG
Alw44I 1154 GTGCAC G^TGCAC
Aor51HI 1505 AGCGCT AGC^GCT
ApaLI 1154 GTGCAC G^TGCAC
AseI 1069 ATTAAT AT^TAAT
AsnI 1069 ATTAAT AT^TAAT
AvaI 810 CCCGGG C^[CT]CG[GA]G
BalI 1718 TGGCCA TGG^CCA
BamHI 1898 GGATCC G^GATCC
BanII 1184 GAGCTC G[GA]GC[CT]^C
BciVI 230 GGATAC GTATCC(6/5)
BfrI 1903 CTTAAG C^TTAAG
BsiYI 948 CCTGAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 948 CCTGAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1401 GGGAC GGGAC(10/14)
BsoBI 810 CCCGGG C^[CT]CG[GA]G
BspMI 1814 ACCTGC ACCTGC(4/8)
BstXI 1560 CCAAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1506 GCGC GCG^C
EaeI 1718 TGGCCA [CT]^GGCC[GA]
Eco47III 1505 AGCGCT AGC^GCT
EcoRV 1828 GATATC GAT^ATC
HaeII 1505 AGCGCT [GA]GCGC^[CT]
HhaI 1506 GCGC GCG^C
HinP1I 1506 GCGC G^CGC
HincII 2091 GTCAAC GT[CT]^[GA]AC
HindII 2091 GTCAAC GT[CT]^[GA]AC
HindIII 1106 AAGCTT A^AGCTT
MaeII 1998 ACGT A^CGT
MfeI 346 CAATTG C^AATTG
MluNI 1718 TGGCCA TGG^CCA
MscI 1718 TGGCCA TGG^CCA
MunI 346 CAATTG C^AATTG
PshBI 1069 ATTAAT AT^TAAT
PstI 389 CTGCAG CTGCA^G
SacI 1184 GAGCTC GAGCT^C
SmaI 810 CCCGGG CCC^GGG
XmaCI 810 CCCGGG C^CCGGG
XmaI 810 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccIII 6 TCCGGA T^CCGGA
1895 TCCGGA
AlwNI 723 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
943 CAGTACCTG
Asp700I 416 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
427 GAAGTCTTTC
AspHI 1154 GTGCAC G[AT]GC[AT]^C
1184 GAGCTC
BcnI 810 CCCGG CC^[GC]GG
811 CCGGG
BglII 715 AGATCT A^GATCT
1605 AGATCT
BmyI 1154 GTGCAC G[AGT]GC[ACT]^C
1184 GAGCTC
BsaWI 6 TCCGGA [AT]^CCGG[AT]
1895 TCCGGA
BseAI 6 TCCGGA T^CCGGA
1895 TCCGGA
BsiHKAI 1154 GTGCAC G[AT]GC[AT]^C
1184 GAGCTC
BsmI 585 GAATGC GAATGC(1/-1)
1636 GCATTC
Bsp1286I 1154 GTGCAC G[AGT]GC[ACT]^C
1184 GAGCTC
BspEI 6 TCCGGA T^CCGGA
1895 TCCGGA
BstNI 108 CCTGG CC^[AT]GG
551 CCTGG
Cac8I 1429 GCAAGC GC[ACGT]^[ACGT]GC
1756 GCTGGC
DraII 815 GGGTCCT [GA]G^G[ACGT]CC[CT]
1868 AGGACCT
EcoO109I 815 GGGTCCT [GA]G^G[ACGT]CC[CT]
1868 AGGACCT
EcoRII 108 CCTGG ^CC[AT]GG
551 CCTGG
EcoT22I 1057 ATGCAT ATGCA^T
1244 ATGCAT
HaeIII 1719 GGCC GG^CC
1990 GGCC
MroI 6 TCCGGA T^CCGGA
1895 TCCGGA
MslI 1290 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1666 CATTTGGATG
MspA1I 1041 CAGCTG C[AC]G^C[GT]G
1690 CAGCTG
MvaI 108 CCTGG CC^[AT]GG
551 CCTGG
NciI 810 CCCGG CC^[GC]GG
811 CCGGG
NsiI 1057 ATGCAT ATGCA^T
1244 ATGCAT
NspI 136 ACATGC [GA]CATG^[CT]
1076 ACATGC
PpuMI 815 GGGTCCT [GA]G^G[AT]CC[CT]
1868 AGGACCT
PvuII 1041 CAGCTG CAG^CTG
1690 CAGCTG
SspI 486 AATATT AAT^ATT
1924 AATATT
XmnI 416 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
427 GAAGTCTTTC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BpuAI
BsaJI BsgI BsmAI BsrDI BsrI
BssKI BstF5I BstYI Cfr13I DdeI
DpnII DraI EcoT14I Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MspI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII