Restriction map for
pFN21AB6233
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AclI 1287 AACGTT AA^CGTT
AflIII 1412 ACGTGT A^C[GA][CT]GT
AspHI 430 GAGCAC G[AT]GC[AT]^C
BanI 1777 GGCACC G^G[CT][GA]CC
BanII 760 GGGCTC G[GA]GC[CT]^C
BglI 1307 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1333 GAGACC GGTCTC(1/5)
BsgI 460 GTGCAG GTGCAG(16/14)
BsiHKAI 430 GAGCAC G[AT]GC[AT]^C
BsiWI 1511 CGTACG C^GTACG
BsmFI 2198 GTCCC GGGAC(10/14)
BsrFI 1892 GCCGGT [GA]^CCGG[CT]
BstAPI 594 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1591 GGTCACC G^GT[ACGT]ACC
BstPI 1591 GGTCACC G^GT[ACGT]ACC
BstXI 1322 CCATAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1892 GCCGGT [GA]^CCGG[CT]
EcoO65I 1591 GGTCACC G^GT[ACGT]ACC
EcoT22I 295 ATGCAT ATGCA^T
HapII 1893 CCGG C^CGG
HindIII 1561 AAGCTT A^AGCTT
HpaII 1893 CCGG C^CGG
MspI 1893 CCGG C^CGG
NsiI 295 ATGCAT ATGCA^T
PpuMI 263 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1287 AACGTT AA^CGTT
SapI 697 GAAGAGC GCTCTTC(1/4)
SexAI 1629 ACCTGGT A^CC[AT]GGT
XbaI 378 TCTAGA T^CTAGA
XcmI 1839 CCATTCTGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BglII 495 AGATCT A^GATCT
1301 AGATCT
BmyI 430 GAGCAC G[AGT]GC[ACT]^C
760 GGGCTC
BpmI 1232 CTGGAG CTGGAG(16/14)
1483 CTGGAG
BseRI 336 GAGGAG GAGGAG(10/8)
838 GAGGAG
Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C
760 GGGCTC
BspMI 1461 ACCTGC ACCTGC(4/8)
48 GCAGGT
BsrDI 1343 CATTGC GCAATG(2/0)
2218 GCAATG
DraII 263 AGGTCCT [GA]G^G[ACGT]CC[CT]
372 GGGGCCT
DraIII 433 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1258 CACATGGTG
EarI 331 GAAGAG CTCTTC(1/4)
697 GAAGAG
EcoO109I 263 AGGTCCT [GA]G^G[ACGT]CC[CT]
372 GGGGCCT
HincII 1416 GTCAAC GT[CT]^[GA]AC
1475 GTCAAC
HindII 1416 GTCAAC GT[CT]^[GA]AC
1475 GTCAAC
Ksp632I 331 GAAGAG CTCTTC(1/4)
697 GAAGAG
MslI 876 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1827 CACCTGGGTG
NdeI 860 CATATG CA^TATG
1546 CATATG
PstI 350 CTGCAG CTGCA^G
592 CTGCAG
SspI 342 AATATT AAT^ATT
1668 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BfaI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MunI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI