Restriction map for
pFN21AE3948
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AvaI 929 CCCGGG C^[CT]CG[GA]G
AvrII 546 CCTAGG C^CTAGG
BanII 14 GGGCTC G[GA]GC[CT]^C
BbsI 900 GAAGAC GAAGAC(2/6)
BbvCI 576 GCTGAGG CCTCAGC(-5/-2)
BglII 198 AGATCT A^GATCT
BlnI 546 CCTAGG C^CTAGG
BpuAI 900 GAAGAC GAAGAC(2/6)
BseRI 999 GAGGAG GAGGAG(10/8)
BsoBI 929 CCCGGG C^[CT]CG[GA]G
BsrBI 104 CCGCTC CCGCTC(-3/-3)
BstYI 198 AGATCT [GA]^GATC[CT]
DpnII 199 GATC ^GATC
EaeI 38 TGGCCG [CT]^GGCC[GA]
EcoT14I 546 CCTAGG C^C[AT][AT]GG
HapII 930 CCGG C^CGG
HincII 602 GTCGAC GT[CT]^[GA]AC
HindII 602 GTCGAC GT[CT]^[GA]AC
HindIII 1010 AAGCTT A^AGCTT
HpaII 930 CCGG C^CGG
MboI 199 GATC ^GATC
MflI 198 AGATCT [GA]^GATC[CT]
MspI 930 CCGG C^CGG
NdeII 199 GATC ^GATC
NspI 114 GCATGT [GA]CATG^[CT]
PleI 1002 GAGTC GAGTC(4/5)
PvuII 26 CAGCTG CAG^CTG
SalI 602 GTCGAC G^TCGAC
SapI 106 GCTCTTC GCTCTTC(1/4)
Sau3AI 199 GATC ^GATC
SmaI 929 CCCGGG CCC^GGG
SmlI 408 CTTGAG C^T[CT][GA]AG
SspI 425 AATATT AAT^ATT
StyI 546 CCTAGG C^C[AT][AT]GG
Tsp45I 1004 GTCAC ^GT[GC]AC
Tsp509I 273 AATT ^AATT
XbaI 226 TCTAGA T^CTAGA
XcmI 56 CCATCTTTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 198 AGATCT [GA]^GATC[CT]
XmaCI 929 CCCGGG C^CCGGG
XmaI 929 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 602 GTCGAC GT^[AC][GT]AC
741 GTCTAC
AspHI 7 GAGCAC G[AT]GC[AT]^C
634 GAGCAC
BcnI 929 CCCGG CC^[GC]GG
930 CCGGG
BlpI 4 GCTGAGC GC^T[ACGT]AGC
556 GCTCAGC
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
556 GCTCAGC
BsaI 99 GAGACC GGTCTC(1/5)
319 GGTCTC
BsiHKAI 7 GAGCAC G[AT]GC[AT]^C
634 GAGCAC
BsiYI 750 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1018 CCCATCACAGG
BslI 750 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1018 CCCATCACAGG
BsmFI 284 GGGAC GGGAC(10/14)
1016 GTCCC
Bsp1407I 525 TGTACA T^GTACA
855 TGTACA
BsrGI 525 TGTACA T^GTACA
855 TGTACA
Cac8I 582 GCTGGC GC[ACGT]^[ACGT]GC
652 GCCAGC
CelII 4 GCTGAGC GC^T[ACGT]AGC
556 GCTCAGC
EarI 107 CTCTTC CTCTTC(1/4)
415 GAAGAG
FauI 103 CCCGC CCCGC(4/6)
449 CCCGC
Ksp632I 107 CTCTTC CTCTTC(1/4)
415 GAAGAG
MaeIII 974 GTTAC ^GT[ACGT]AC
1004 GTCAC
NciI 929 CCCGG CC^[GC]GG
930 CCGGG
SfaNI 660 GATGC GCATC(5/9)
561 GCATC
SspBI 525 TGTACA T^GTACA
855 TGTACA
TaqI 537 TCGA T^CGA
603 TCGA
TfiI 136 GAATC G^A[AT]TC
877 GATTC
TthHB8I 537 TCGA T^CGA
603 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BmyI BpmI BsaJI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MboII MnlI
MseI MspA1I MvaI MwoI NlaIII
RsaI ScrFI Tru9I TseI TspRI