Restriction map for
pFN21AE2266
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BlnI BsaHI BseRI
BsgI BsiEI BsiWI BspDI BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MfeI MluI MunI MvnI NarI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 803 TCCGGA T^CCGGA
AseI 1362 ATTAAT AT^TAAT
AsnI 1362 ATTAAT AT^TAAT
Asp700I 1998 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 14 CCCGAG C^[CT]CG[GA]G
BalI 1241 TGGCCA TGG^CCA
BciVI 1958 GTATCC GTATCC(6/5)
BclI 2309 TGATCA T^GATCA
BglII 91 AGATCT A^GATCT
BpmI 2012 CTGGAG CTGGAG(16/14)
BsaAI 2282 CACGTA [CT]AC^GT[GA]
BsaI 1294 GAGACC GGTCTC(1/5)
BsaWI 803 TCCGGA [AT]^CCGG[AT]
BseAI 803 TCCGGA T^CCGGA
BsoBI 14 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2238 TGTACA T^GTACA
BspEI 803 TCCGGA T^CCGGA
BspLU11I 276 ACATGT A^CATGT
BspMI 1528 GCAGGT ACCTGC(4/8)
BsrFI 1226 GCCGGC [GA]^CCGG[CT]
BsrGI 2238 TGTACA T^GTACA
BstBI 1418 TTCGAA TT^CGAA
Cfr10I 1226 GCCGGC [GA]^CCGG[CT]
DraIII 1938 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1705 GACTGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1535 CCATGG C^C[GA][CT]GG
EcoRI 823 GAATTC G^AATTC
FbaI 2309 TGATCA T^GATCA
HaeII 1235 AGCGCC [GA]GCGC^[CT]
HpaI 1924 GTTAAC GTT^AAC
MluNI 1241 TGGCCA TGG^CCA
MroI 803 TCCGGA T^CCGGA
MscI 1241 TGGCCA TGG^CCA
NaeI 1226 GCCGGC GCC^GGC
NcoI 1535 CCATGG C^CATGG
NgoMIV 1226 GCCGGC G^CCGGC
NspV 1418 TTCGAA TT^CGAA
PshBI 1362 ATTAAT AT^TAAT
SapI 8 GAAGAGC GCTCTTC(1/4)
SfuI 1418 TTCGAA TT^CGAA
SphI 2204 GCATGC GCATG^C
SspBI 2238 TGTACA T^GTACA
XbaI 406 TCTAGA T^CTAGA
XcmI 260 CCAGAGAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1998 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 701 AAATTC [GA]^AATT[CT]
823 GAATTC
AlwNI 1234 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2128 CAGCTGCTG
ApoI 701 AAATTC [GA]^AATT[CT]
823 GAATTC
BanI 475 GGTGCC G^G[CT][GA]CC
991 GGCACC
BbsI 1665 GAAGAC GAAGAC(2/6)
613 GTCTTC
BcnI 2328 CCGGG CC^[GC]GG
2440 CCGGG
BlpI 1108 GCTCAGC GC^T[ACGT]AGC
2133 GCTGAGC
Bpu1102I 1108 GCTCAGC GC^T[ACGT]AGC
2133 GCTGAGC
BpuAI 1665 GAAGAC GAAGAC(2/6)
613 GTCTTC
BsaBI 558 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1764 GATGAGTATC
BsmBI 1210 CGTCTC CGTCTC(1/5)
811 GAGACG
BsrDI 83 CATTGC GCAATG(2/0)
128 GCAATG
BstXI 53 CCACAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1244 CCAGGCTTGTGG
CelII 1108 GCTCAGC GC^T[ACGT]AGC
2133 GCTGAGC
CfoI 66 GCGC GCG^C
1236 GCGC
DraI 1434 TTTAAA TTT^AAA
1839 TTTAAA
EcoRV 177 GATATC GAT^ATC
1323 GATATC
EcoT14I 1409 CCAAGG C^C[AT][AT]GG
1535 CCATGG
HhaI 66 GCGC GCG^C
1236 GCGC
HinP1I 66 GCGC G^CGC
1236 GCGC
HincII 1382 GTCAAC GT[CT]^[GA]AC
1924 GTTAAC
HindII 1382 GTCAAC GT[CT]^[GA]AC
1924 GTTAAC
MamI 558 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1764 GATGAGTATC
NciI 2328 CCGGG CC^[GC]GG
2440 CCGGG
NdeI 1377 CATATG CA^TATG
2392 CATATG
NspI 276 ACATGT [GA]CATG^[CT]
2204 GCATGC
SacI 1132 GAGCTC GAGCT^C
1698 GAGCTC
SexAI 1456 ACCTGGT A^CC[AT]GGT
1939 ACCAGGT
SmlI 1515 CTTGAG C^T[CT][GA]AG
2169 CTCAAG
SspI 1359 AATATT AAT^ATT
1691 AATATT
StyI 1409 CCAAGG C^C[AT][AT]GG
1535 CCATGG
TaqI 470 TCGA T^CGA
1419 TCGA
TthHB8I 470 TCGA T^CGA
1419 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BanII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII