Restriction map for
pFN21AE4506
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV FauI FseI FspI
HaeII HapII HgaI HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI SmlI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 436 GACGTC GACGT^C
AciI 209 CCGC CCGC(-3/-1)
AcyI 436 GACGTC G[GA]^CG[CT]C
AseI 1297 ATTAAT AT^TAAT
AsnI 1297 ATTAAT AT^TAAT
AspHI 891 GAGCTC G[AT]GC[AT]^C
BamHI 1160 GGATCC G^GATCC
BanII 891 GAGCTC G[GA]GC[CT]^C
BciVI 1067 GGATAC GTATCC(6/5)
BlpI 481 GCTGAGC GC^T[ACGT]AGC
BmyI 891 GAGCTC G[AGT]GC[ACT]^C
BpmI 896 CTGGAG CTGGAG(16/14)
Bpu1102I 481 GCTGAGC GC^T[ACGT]AGC
BsaAI 577 TACGTA [CT]AC^GT[GA]
BsaBI 1512 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 436 GACGTC G[GA]^CG[CT]C
BsaI 1042 GGTCTC GGTCTC(1/5)
BsiHKAI 891 GAGCTC G[AT]GC[AT]^C
BsmI 1278 GAATGC GAATGC(1/-1)
Bsp1286I 891 GAGCTC G[AGT]GC[ACT]^C
BspHI 150 TCATGA T^CATGA
BspMI 849 ACCTGC ACCTGC(4/8)
BsrBI 209 CCGCTC CCGCTC(-3/-3)
BsrDI 1206 CATTGC GCAATG(2/0)
Cac8I 477 GCTGGC GC[ACGT]^[ACGT]GC
CelII 481 GCTGAGC GC^T[ACGT]AGC
CfoI 1404 GCGC GCG^C
DsaI 1155 CCATGG C^C[GA][CT]GG
EarI 1287 GAAGAG CTCTTC(1/4)
EcoNI 861 CCTGCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1340 GAATTC G^AATTC
EcoT14I 1155 CCATGG C^C[AT][AT]GG
EcoT22I 1280 ATGCAT ATGCA^T
HhaI 1404 GCGC GCG^C
Hin1I 436 GACGTC G[GA]^CG[CT]C
HinP1I 1404 GCGC G^CGC
Ksp632I 1287 GAAGAG CTCTTC(1/4)
MamI 1512 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1232 CAGCTG C[AC]G^C[GT]G
NcoI 1155 CCATGG C^CATGG
NsiI 1280 ATGCAT ATGCA^T
PflMI 1464 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1297 ATTAAT AT^TAAT
PvuII 1232 CAGCTG CAG^CTG
RcaI 150 TCATGA T^CATGA
SacI 891 GAGCTC GAGCT^C
SexAI 1450 ACCTGGT A^CC[AT]GGT
SfcI 465 CTGTAG C^T[GA][CT]AG
SnaBI 577 TACGTA TAC^GTA
StyI 1155 CCATGG C^C[AT][AT]GG
TfiI 1176 GATTC G^A[AT]TC
Van91I 1464 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 865 CCTAAGG CC^T[ACGT]AGG
1037 CCTTAGG
BclI 606 TGATCA T^GATCA
1350 TGATCA
Bsp1407I 506 TGTACA T^GTACA
885 TGTACA
BsrGI 506 TGTACA T^GTACA
885 TGTACA
BstBI 670 TTCGAA TT^CGAA
773 TTCGAA
BstYI 989 AGATCC [GA]^GATC[CT]
1160 GGATCC
Bsu36I 865 CCTAAGG CC^T[ACGT]AGG
1037 CCTTAGG
Cfr13I 959 GGGCC G^G[ACGT]CC
984 GGCCC
Eco81I 865 CCTAAGG CC^T[ACGT]AGG
1037 CCTTAGG
FbaI 606 TGATCA T^GATCA
1350 TGATCA
MflI 989 AGATCC [GA]^GATC[CT]
1160 GGATCC
NspV 670 TTCGAA TT^CGAA
773 TTCGAA
PleI 836 GACTC GAGTC(4/5)
424 GAGTC
Sau96I 959 GGGCC G^G[ACGT]CC
984 GGCCC
SfuI 670 TTCGAA TT^CGAA
773 TTCGAA
SspBI 506 TGTACA T^GTACA
885 TGTACA
SspI 351 AATATT AAT^ATT
675 AATATT
TspRI 841 CACTG CA[GC]TG[ACGT][ACGT]^
1358 CAGTG
XhoII 989 AGATCC [GA]^GATC[CT]
1160 GGATCC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BpuAI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI TaqI Tru9I TseI
Tsp45I Tsp509I TthHB8I