Restriction map for
pFN21AE4515
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AlwNI 694 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1069 CCTGAGG CC^T[ACGT]AGG
AvaI 835 CTCGGG C^[CT]CG[GA]G
BamHI 751 GGATCC G^GATCC
BbvCI 1551 CCTCAGC CCTCAGC(-5/-2)
BcnI 1518 CCCGG CC^[GC]GG
BlpI 1092 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1092 GCTGAGC GC^T[ACGT]AGC
BseRI 1217 GAGGAG GAGGAG(10/8)
BsmFI 530 GTCCC GGGAC(10/14)
BsoBI 835 CTCGGG C^[CT]CG[GA]G
BspHI 597 TCATGA T^CATGA
Bst1107I 429 GTATAC GTA^TAC
BstAPI 817 GCAATGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 785 CCAATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 751 GGATCC [GA]^GATC[CT]
BstZ17I 429 GTATAC GTA^TAC
Bsu36I 1069 CCTGAGG CC^T[ACGT]AGG
CelII 1092 GCTGAGC GC^T[ACGT]AGC
CfoI 303 GCGC GCG^C
DraII 695 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 781 GACACCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 14 CCACGG C^C[GA][CT]GG
EaeI 11 CGGCCA [CT]^GGCC[GA]
Eco81I 1069 CCTGAGG CC^T[ACGT]AGG
EcoO109I 695 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1141 GAATTC G^AATTC
EcoT22I 1241 ATGCAT ATGCA^T
HapII 1519 CCGG C^CGG
HhaI 303 GCGC GCG^C
HinP1I 303 GCGC G^CGC
HincII 322 GTTGAC GT[CT]^[GA]AC
HindII 322 GTTGAC GT[CT]^[GA]AC
HpaII 1519 CCGG C^CGG
HphI 438 GGTGA GGTGA(8/7)
MflI 751 GGATCC [GA]^GATC[CT]
MslI 1485 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1025 CAGCTG C[AC]G^C[GT]G
MspI 1519 CCGG C^CGG
NciI 1518 CCCGG CC^[GC]GG
NheI 813 GCTAGC G^CTAGC
NsiI 1241 ATGCAT ATGCA^T
NspI 155 GCATGT [GA]CATG^[CT]
PflMI 1613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 590 CTGCAG CTGCA^G
PvuII 1025 CAGCTG CAG^CTG
RcaI 597 TCATGA T^CATGA
SfcI 590 CTGCAG C^T[GA][CT]AG
Van91I 1613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 751 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 103 GTCTAC GT^[AC][GT]AC
429 GTATAC
Asp700I 879 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
928 GAAGCCCTTC
AspHI 144 GAGCAC G[AT]GC[AT]^C
1095 GAGCAC
BbsI 830 GAAGAC GAAGAC(2/6)
1534 GTCTTC
BglI 697 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1489 GCCATGCTGGC
BpuAI 830 GAAGAC GAAGAC(2/6)
1534 GTCTTC
BsaAI 187 TACGTG [CT]AC^GT[GA]
1302 TACGTG
BsiHKAI 144 GAGCAC G[AT]GC[AT]^C
1095 GAGCAC
Bsp1407I 797 TGTACA T^GTACA
1375 TGTACA
BsrDI 666 CATTGC GCAATG(2/0)
817 GCAATG
BsrGI 797 TGTACA T^GTACA
1375 TGTACA
EcoT14I 605 CCTTGG C^C[AT][AT]GG
1337 CCTTGG
HgaI 725 GACGC GACGC(5/10)
528 GCGTC
NdeI 950 CATATG CA^TATG
1238 CATATG
PleI 514 GACTC GAGTC(4/5)
833 GACTC
SmlI 34 CTCAAG C^T[CT][GA]AG
141 CTTGAG
SspBI 797 TGTACA T^GTACA
1375 TGTACA
StyI 605 CCTTGG C^C[AT][AT]GG
1337 CCTTGG
Tsp45I 439 GTGAC ^GT[GC]AC
512 GTGAC
XcmI 14 CCACGGGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1609 CCATCCAGTCTGTGG
XmnI 879 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
928 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HindIII HinfI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI