Restriction map for
pFN21AE9665
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeII
MamI MluI MroI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Alw44I 1497 GTGCAC G^TGCAC
Aor51HI 1701 AGCGCT AGC^GCT
ApaLI 1497 GTGCAC G^TGCAC
Asp700I 1968 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1158 TGGCCA TGG^CCA
BanII 490 GAGCTC G[GA]GC[CT]^C
BbsI 706 GAAGAC GAAGAC(2/6)
BglI 409 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 706 GAAGAC GAAGAC(2/6)
BsaWI 1399 ACCGGA [AT]^CCGG[AT]
BsgI 1275 CTGCAC GTGCAG(16/14)
BsiEI 1397 CGACCG CG[GA][CT]^CG
BsmFI 1035 GTCCC GGGAC(10/14)
BspMI 1661 ACCTGC ACCTGC(4/8)
BsrDI 1188 CATTGC GCAATG(2/0)
BstAPI 510 GCATCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1129 TTCGAA TT^CGAA
DraI 783 TTTAAA TTT^AAA
DraII 323 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1701 AGCGCT AGC^GCT
EcoNI 1542 CCTCATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 323 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1584 GATATC GAT^ATC
FauI 345 GCGGG CCCGC(4/6)
HgaI 88 GCGTC GACGC(5/10)
HincII 733 GTTGAC GT[CT]^[GA]AC
HindII 733 GTTGAC GT[CT]^[GA]AC
MfeI 581 CAATTG C^AATTG
MluNI 1158 TGGCCA TGG^CCA
MscI 1158 TGGCCA TGG^CCA
MunI 581 CAATTG C^AATTG
NspI 588 GCATGT [GA]CATG^[CT]
NspV 1129 TTCGAA TT^CGAA
PflMI 1161 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 323 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1398 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 305 CAGCTG CAG^CTG
SacI 490 GAGCTC GAGCT^C
SapI 1756 GAAGAGC GCTCTTC(1/4)
SbfI 522 CCTGCAGG CCTGCA^GG
SexAI 153 ACCAGGT A^CC[AT]GGT
SfuI 1129 TTCGAA TT^CGAA
Sse8387I 522 CCTGCAGG CCTGCA^GG
StuI 281 AGGCCT AGG^CCT
Van91I 1161 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1752 TCTAGA T^CTAGA
XcmI 1580 CCAAGATATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1968 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 292 GTAC GT^AC
1171 GTAC
AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
526 CAGGAGCTG
AvaII 324 GGTCC G^G[AT]CC
1034 GGTCC
BamHI 1517 GGATCC G^GATCC
1538 GGATCC
BanI 48 GGCGCC G^G[CT][GA]CC
1456 GGCGCC
BbeI 48 GGCGCC GGCGC^C
1456 GGCGCC
BfaI 161 CTAG C^TAG
1753 CTAG
BpmI 31 CTGGAG CTGGAG(16/14)
94 CTGGAG
BsaI 1002 GGTCTC GGTCTC(1/5)
1024 GGTCTC
BsmBI 89 CGTCTC CGTCTC(1/5)
1393 GAGACG
Cfr13I 324 GGTCC G^G[ACGT]CC
1034 GGTCC
EaeI 41 CGGCCA [CT]^GGCC[GA]
1158 TGGCCA
EarI 634 GAAGAG CTCTTC(1/4)
1756 GAAGAG
EcoRI 298 GAATTC G^AATTC
945 GAATTC
HapII 52 CCGG C^CGG
1400 CCGG
HpaII 52 CCGG C^CGG
1400 CCGG
KasI 48 GGCGCC G^GCGCC
1456 GGCGCC
Ksp632I 634 GAAGAG CTCTTC(1/4)
1756 GAAGAG
MaeI 161 CTAG C^TAG
1753 CTAG
MspI 52 CCGG C^CGG
1400 CCGG
NarI 48 GGCGCC GG^CGCC
1456 GGCGCC
PstI 523 CTGCAG CTGCA^G
1384 CTGCAG
RsaI 292 GTAC GT^AC
1171 GTAC
Sau96I 324 GGTCC G^G[ACGT]CC
1034 GGTCC
SfaNI 1324 GATGC GCATC(5/9)
510 GCATC
SfoI 48 GGCGCC GGC^GCC
1456 GGCGCC
SmlI 328 CTTGAG C^T[CT][GA]AG
455 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
ApoI AspHI BbvI BmyI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
Hin1I HinP1I HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
Sau3AI ScrFI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII