Restriction map for
pFN21AE1089
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaBI BsaHI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BspDI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT14I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 369 TCCGGA T^CCGGA
AhdI 1258 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 913 CCTAAGG CC^T[ACGT]AGG
AspEI 1258 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1149 CCCGGG C^[CT]CG[GA]G
BalI 1136 TGGCCA TGG^CCA
BanI 454 GGCACC G^G[CT][GA]CC
BbvCI 1339 CCTCAGC CCTCAGC(-5/-2)
BbvI 1284 GCTGC GCAGC(8/12)
BfaI 148 CTAG C^TAG
BlpI 1154 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1154 GCTTAGC GC^T[ACGT]AGC
BsaAI 1320 TACGTA [CT]AC^GT[GA]
BsaI 171 GAGACC GGTCTC(1/5)
BseAI 369 TCCGGA T^CCGGA
BsoBI 1149 CCCGGG C^[CT]CG[GA]G
Bsp1407I 464 TGTACA T^GTACA
BspEI 369 TCCGGA T^CCGGA
BspMI 773 ACCTGC ACCTGC(4/8)
BsrDI 1164 CATTGC GCAATG(2/0)
BsrGI 464 TGTACA T^GTACA
BstXI 128 CCAGGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 913 CCTAAGG CC^T[ACGT]AGG
CelII 1154 GCTTAGC GC^T[ACGT]AGC
DraII 315 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1136 TGGCCA [CT]^GGCC[GA]
Eam1105I 1258 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 738 GAAGAG CTCTTC(1/4)
Eco81I 913 CCTAAGG CC^T[ACGT]AGG
EcoNI 909 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 315 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 211 GATATC GAT^ATC
FauI 1281 CCCGC CCCGC(4/6)
Fnu4HI 1284 GCTGC GC^[ACGT]GC
ItaI 1284 GCTGC GC^[ACGT]GC
Ksp632I 738 GAAGAG CTCTTC(1/4)
MaeI 148 CTAG C^TAG
MluNI 1136 TGGCCA TGG^CCA
MroI 369 TCCGGA T^CCGGA
MscI 1136 TGGCCA TGG^CCA
MslI 414 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1282 CCGCTG C[AC]G^C[GT]G
PflMI 246 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 315 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 80 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 1149 CCCGGG CCC^GGG
SnaBI 1320 TACGTA TAC^GTA
SspBI 464 TGTACA T^GTACA
TseI 1284 GCTGC G^C[AT]GC
Tsp45I 220 GTCAC ^GT[GC]AC
Van91I 246 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1149 CCCGGG C^CCGGG
XmaI 1149 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 673 GTAGAC GT^[AC][GT]AC
1309 GTCTAC
AciI 1282 CCGC CCGC(-3/-1)
438 GCGG
AcsI 307 AAATTT [GA]^AATT[CT]
547 AAATTT
ApoI 307 AAATTT [GA]^AATT[CT]
547 AAATTT
AspHI 54 GAGCAC G[AT]GC[AT]^C
868 GTGCTC
BamHI 41 GGATCC G^GATCC
1074 GGATCC
BbsI 204 GAAGAC GAAGAC(2/6)
121 GTCTTC
BcnI 1149 CCCGG CC^[GC]GG
1150 CCGGG
BmyI 54 GAGCAC G[AGT]GC[ACT]^C
868 GTGCTC
BpuAI 204 GAAGAC GAAGAC(2/6)
121 GTCTTC
BsaJI 1139 CCAGGG C^C[ACGT][ACGT]GG
1149 CCCGGG
BsaWI 332 TCCGGT [AT]^CCGG[AT]
369 TCCGGA
BsiHKAI 54 GAGCAC G[AT]GC[AT]^C
868 GTGCTC
BsmAI 171 GAGAC GTCTC(1/5)
808 GTCTC
Bsp1286I 54 GAGCAC G[AGT]GC[ACT]^C
868 GTGCTC
BstYI 41 GGATCC [GA]^GATC[CT]
1074 GGATCC
DraI 310 TTTAAA TTT^AAA
1326 TTTAAA
MaeII 639 ACGT A^CGT
1321 ACGT
MflI 41 GGATCC [GA]^GATC[CT]
1074 GGATCC
NciI 1149 CCCGG CC^[GC]GG
1150 CCGGG
TfiI 795 GATTC G^A[AT]TC
1295 GAATC
XhoII 41 GGATCC [GA]^GATC[CT]
1074 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI AlwI AvaII BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT22I FokI HaeIII HapII HinfI
HpaII HphI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NsiI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I Tsp509I TspRI