Restriction map for
pFN21AE6414
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DpnII DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI Ksp632I
KspI MamI MboI MflI MluI
MroI MvnI NarI NciI NdeII
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau3AI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 548 GTCTAC GT^[AC][GT]AC
AciI 389 CCGC CCGC(-3/-1)
AflII 471 CTTAAG C^TTAAG
Alw44I 1100 GTGCAC G^TGCAC
ApaLI 1100 GTGCAC G^TGCAC
AseI 903 ATTAAT AT^TAAT
AsnI 903 ATTAAT AT^TAAT
AspHI 1100 GTGCAC G[AT]GC[AT]^C
AvrII 774 CCTAGG C^CTAGG
BalI 446 TGGCCA TGG^CCA
BbsI 1329 GAAGAC GAAGAC(2/6)
BfrI 471 CTTAAG C^TTAAG
BglI 562 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 774 CCTAGG C^CTAGG
BpuAI 1329 GAAGAC GAAGAC(2/6)
BsaI 585 GAGACC GGTCTC(1/5)
BseRI 1169 CTCCTC GAGGAG(10/8)
BsiEI 256 CGGCCG CG[GA][CT]^CG
BsiHKAI 1100 GTGCAC G[AT]GC[AT]^C
BsmAI 585 GAGAC GTCTC(1/5)
Bsp1407I 1217 TGTACA T^GTACA
BspHI 581 TCATGA T^CATGA
BsrDI 1271 GCAATG GCAATG(2/0)
BsrGI 1217 TGTACA T^GTACA
BssKI 1017 CCTGG ^CC[ACGT]GG
BssSI 1093 CTCGTG CACGAG(-5/-1)
BstNI 1017 CCTGG CC^[AT]GG
BstXI 188 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 216 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1400 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 256 CGGCCG C^GGCCG
EclXI 256 CGGCCG C^GGCCG
Eco52I 256 CGGCCG C^GGCCG
EcoO109I 216 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 935 GAATTC G^AATTC
EcoRII 1017 CCTGG ^CC[AT]GG
EcoT22I 101 ATGCAT ATGCA^T
FauI 388 CCCGC CCCGC(4/6)
HincII 1378 GTTAAC GT[CT]^[GA]AC
HindII 1378 GTTAAC GT[CT]^[GA]AC
HindIII 528 AAGCTT A^AGCTT
HpaI 1378 GTTAAC GTT^AAC
MaeII 1382 ACGT A^CGT
MfeI 1136 CAATTG C^AATTG
MluNI 446 TGGCCA TGG^CCA
MscI 446 TGGCCA TGG^CCA
MspA1I 331 CAGCTG C[AC]G^C[GT]G
MunI 1136 CAATTG C^AATTG
MvaI 1017 CCTGG CC^[AT]GG
NaeI 254 GCCGGC GCC^GGC
NdeI 809 CATATG CA^TATG
NgoMIV 254 GCCGGC G^CCGGC
NsiI 101 ATGCAT ATGCA^T
PpuMI 216 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 903 ATTAAT AT^TAAT
PvuII 331 CAGCTG CAG^CTG
RcaI 581 TCATGA T^CATGA
ScrFI 1017 CCTGG CC^[ACGT]GG
SfaNI 30 GCATC GCATC(5/9)
SspBI 1217 TGTACA T^GTACA
SspI 147 AATATT AAT^ATT
StuI 889 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 137 AAATTC [GA]^AATT[CT]
935 GAATTC
AflIII 1214 ACATGT A^C[GA][CT]GT
1220 ACATGT
ApoI 137 AAATTC [GA]^AATT[CT]
935 GAATTC
BfaI 775 CTAG C^TAG
887 CTAG
BpmI 38 CTCCAG CTGGAG(16/14)
1199 CTGGAG
BsmI 558 GAATGC GAATGC(1/-1)
103 GCATTC
BspLU11I 1214 ACATGT A^CATGT
1220 ACATGT
BspMI 380 ACCTGC ACCTGC(4/8)
1177 GCAGGT
BsrFI 254 GCCGGC [GA]^CCGG[CT]
258 GCCGGT
Cfr10I 254 GCCGGC [GA]^CCGG[CT]
258 GCCGGT
DdeI 614 CTAAG C^T[ACGT]AG
1413 CTCAG
DsaI 431 CCATGG C^C[GA][CT]GG
449 CCATGG
EaeI 256 CGGCCG [CT]^GGCC[GA]
446 TGGCCA
EcoNI 892 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1171 CCTCTTGCAGG
HapII 255 CCGG C^CGG
259 CCGG
HpaII 255 CCGG C^CGG
259 CCGG
MaeI 775 CTAG C^TAG
887 CTAG
MslI 1134 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1215 CATGTACATG
MspI 255 CCGG C^CGG
259 CCGG
NcoI 431 CCATGG C^CATGG
449 CCATGG
NspI 1214 ACATGT [GA]CATG^[CT]
1220 ACATGT
ScaI 836 AGTACT AGT^ACT
941 AGTACT
SmlI 471 CTTAAG C^T[CT][GA]AG
1350 CTTGAG
TaqI 927 TCGA T^CGA
933 TCGA
TthHB8I 927 TCGA T^CGA
933 TCGA
XcmI 40 CCAGATGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
791 CCATTATATTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BstF5I Cac8I Cfr13I EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboII MnlI MseI MwoI
NlaIII NlaIV RsaI Sau96I SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI