Restriction map for
pFN21AB6273
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
StyI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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ApaI 1664 GGGCCC GGGCC^C
Asp700I 1418 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 213 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 560 GGACC G^G[AT]CC
BanI 1714 GGTGCC G^G[CT][GA]CC
BclI 1164 TGATCA T^GATCA
BglII 750 AGATCT A^GATCT
BsaAI 434 TACGTG [CT]AC^GT[GA]
BsaBI 1531 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1257 TCCGGT [AT]^CCGG[AT]
BsmFI 887 GTCCC GGGAC(10/14)
Bsp1407I 528 TGTACA T^GTACA
BsrBI 1743 GAGCGG CCGCTC(-3/-3)
BsrGI 528 TGTACA T^GTACA
BstAPI 1615 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 472 GGTTACC G^GT[ACGT]ACC
BstPI 472 GGTTACC G^GT[ACGT]ACC
BstXI 973 CCAACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1830 TTTAAA TTT^AAA
DsaI 1595 CCACGG C^C[GA][CT]GG
EarI 2307 GAAGAG CTCTTC(1/4)
EcoO65I 472 GGTTACC G^GT[ACGT]ACC
FbaI 1164 TGATCA T^GATCA
HapII 1258 CCGG C^CGG
HpaII 1258 CCGG C^CGG
Ksp632I 2307 GAAGAG CTCTTC(1/4)
MamI 1531 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1258 CCGG C^CGG
NspI 1547 GCATGC [GA]CATG^[CT]
PflFI 213 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1605 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 1726 CCTGCAGG CCTGCA^GG
SphI 1547 GCATGC GCATG^C
Sse8387I 1726 CCTGCAGG CCTGCA^GG
SspBI 528 TGTACA T^GTACA
StuI 2024 AGGCCT AGG^CCT
Tth111I 213 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1605 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1418 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 413 GCGG CCGC(-3/-1)
1745 GCGG
AcsI 845 AAATTT [GA]^AATT[CT]
2332 AAATTT
AocI 1427 CCTCAGG CC^T[ACGT]AGG
1757 CCTCAGG
ApoI 845 AAATTT [GA]^AATT[CT]
2332 AAATTT
BalI 899 TGGCCA TGG^CCA
982 TGGCCA
BanII 1650 GGGCTC G[GA]GC[CT]^C
1664 GGGCCC
BbsI 568 GAAGAC GAAGAC(2/6)
1353 GTCTTC
BpuAI 568 GAAGAC GAAGAC(2/6)
1353 GTCTTC
BsaI 63 GAGACC GGTCTC(1/5)
298 GAGACC
BsgI 648 CTGCAC GTGCAG(16/14)
32 GTGCAG
BsrDI 409 CATTGC GCAATG(2/0)
1792 GCAATG
Bsu36I 1427 CCTCAGG CC^T[ACGT]AGG
1757 CCTCAGG
DraII 1663 GGGGCCC [GA]G^G[ACGT]CC[CT]
1852 AGGCCCT
Eco81I 1427 CCTCAGG CC^T[ACGT]AGG
1757 CCTCAGG
EcoO109I 1663 GGGGCCC [GA]G^G[ACGT]CC[CT]
1852 AGGCCCT
HindIII 8 AAGCTT A^AGCTT
196 AAGCTT
HphI 644 TCACC GGTGA(8/7)
1570 TCACC
MaeII 435 ACGT A^CGT
498 ACGT
MluNI 899 TGGCCA TGG^CCA
982 TGGCCA
MscI 899 TGGCCA TGG^CCA
982 TGGCCA
PstI 1727 CTGCAG CTGCA^G
1903 CTGCAG
SspI 338 AATATT AAT^ATT
400 AATATT
XcmI 1601 CCACCCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1869 CCAGCTTCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AfaI AluI AlwI BbvCI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII