Restriction map for
pFN21AE4566
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanII
BbeI BbvCI BcgI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BsoBI BspDI BspEI BspMI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FseI FspI HaeII HapII HgaI
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1066 GACGTC GACGT^C
AccI 2116 GTCGAC GT^[AC][GT]AC
AcsI 68 AAATTT [GA]^AATT[CT]
AcyI 1066 GACGTC G[GA]^CG[CT]C
AflII 1114 CTTAAG C^TTAAG
ApoI 68 AAATTT [GA]^AATT[CT]
AseI 1648 ATTAAT AT^TAAT
AsnI 1648 ATTAAT AT^TAAT
BamHI 957 GGATCC G^GATCC
BbrPI 2017 CACGTG CAC^GTG
BbsI 94 GAAGAC GAAGAC(2/6)
BciVI 1490 GTATCC GTATCC(6/5)
BfaI 1328 CTAG C^TAG
BfrI 1114 CTTAAG C^TTAAG
BpuAI 94 GAAGAC GAAGAC(2/6)
BsaHI 1066 GACGTC G[GA]^CG[CT]C
BsmBI 1534 GAGACG CGTCTC(1/5)
Bsp1407I 303 TGTACA T^GTACA
BspHI 464 TCATGA T^CATGA
BspLU11I 2084 ACATGT A^CATGT
BsrBI 1126 CCGCTC CCGCTC(-3/-3)
BsrGI 303 TGTACA T^GTACA
BstEII 1272 GGTGACC G^GT[ACGT]ACC
BstPI 1272 GGTGACC G^GT[ACGT]ACC
BstXI 1037 CCAACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 957 GGATCC [GA]^GATC[CT]
Cac8I 2032 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 2101 GCGC GCG^C
DrdI 1924 GACCGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2037 CCACGG C^C[GA][CT]GG
EarI 964 GAAGAG CTCTTC(1/4)
EcoNI 795 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1272 GGTGACC G^GT[ACGT]ACC
EcoT14I 2027 CCAAGG C^C[AT][AT]GG
HhaI 2101 GCGC GCG^C
Hin1I 1066 GACGTC G[GA]^CG[CT]C
HinP1I 2101 GCGC G^CGC
HincII 2116 GTCGAC GT[CT]^[GA]AC
HindII 2116 GTCGAC GT[CT]^[GA]AC
Ksp632I 964 GAAGAG CTCTTC(1/4)
MaeI 1328 CTAG C^TAG
MflI 957 GGATCC [GA]^GATC[CT]
PflMI 636 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2017 CACGTG CAC^GTG
PmlI 2017 CACGTG CAC^GTG
PshBI 1648 ATTAAT AT^TAAT
PstI 1081 CTGCAG CTGCA^G
PvuII 519 CAGCTG CAG^CTG
RcaI 464 TCATGA T^CATGA
SalI 2116 GTCGAC G^TCGAC
ScaI 981 AGTACT AGT^ACT
SspBI 303 TGTACA T^GTACA
SspI 410 AATATT AAT^ATT
StyI 2027 CCAAGG C^C[AT][AT]GG
Van91I 636 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 957 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 23 ACGTGT A^C[GA][CT]GT
2084 ACATGT
AspHI 1106 GTGCTC G[AT]GC[AT]^C
1800 GTGCTC
BalI 1502 TGGCCA TGG^CCA
1559 TGGCCA
BanI 478 GGTGCC G^G[CT][GA]CC
2105 GGTGCC
BclI 938 TGATCA T^GATCA
1610 TGATCA
BmyI 1106 GTGCTC G[AGT]GC[ACT]^C
1800 GTGCTC
BpmI 1208 CTCCAG CTGGAG(16/14)
1249 CTGGAG
BsaAI 874 TACGTG [CT]AC^GT[GA]
2017 CACGTG
BsaI 37 GAGACC GGTCTC(1/5)
1698 GAGACC
BsiHKAI 1106 GTGCTC G[AT]GC[AT]^C
1800 GTGCTC
Bsp1286I 1106 GTGCTC G[AGT]GC[ACT]^C
1800 GTGCTC
BsrDI 1847 CATTGC GCAATG(2/0)
1956 CATTGC
FbaI 938 TGATCA T^GATCA
1610 TGATCA
MluNI 1502 TGGCCA TGG^CCA
1559 TGGCCA
MscI 1502 TGGCCA TGG^CCA
1559 TGGCCA
MseI 1115 TTAA T^TAA
1649 TTAA
MslI 1480 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1543 CATAAAGGTG
MspA1I 519 CAGCTG C[AC]G^C[GT]G
1155 CAGCGG
NspI 1745 ACATGC [GA]CATG^[CT]
2084 ACATGT
SfaNI 1291 GATGC GCATC(5/9)
815 GCATC
SmlI 160 CTTGAG C^T[CT][GA]AG
1114 CTTAAG
StuI 119 AGGCCT AGG^CCT
1668 AGGCCT
TaqI 1130 TCGA T^CGA
2117 TCGA
Tru9I 1115 TTAA T^TAA
1649 TTAA
TthHB8I 1130 TCGA T^CGA
2117 TCGA
XcmI 329 CCAGAGTGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1238 CCAAAGAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstUI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfcI TfiI TseI Tsp45I
Tsp509I TspRI