Restriction map for
pFN21AE5409
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI MamI MluI
MluNI MroI MscI MslI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 688 GTATAC GT^[AC][GT]AC
AflIII 1042 ACGTGT A^C[GA][CT]GT
Alw44I 220 GTGCAC G^TGCAC
ApaI 235 GGGCCC GGGCC^C
ApaLI 220 GTGCAC G^TGCAC
AspHI 220 GTGCAC G[AT]GC[AT]^C
AspI 99 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 371 CCCGAG C^[CT]CG[GA]G
BamHI 151 GGATCC G^GATCC
BanII 235 GGGCCC G[GA]GC[CT]^C
BpmI 302 CTCCAG CTGGAG(16/14)
BsaI 165 GGTCTC GGTCTC(1/5)
BsaWI 148 ACCGGA [AT]^CCGG[AT]
BsiHKAI 220 GTGCAC G[AT]GC[AT]^C
BsoBI 371 CCCGAG C^[CT]CG[GA]G
BspMI 669 ACCTGC ACCTGC(4/8)
BsrBI 204 GAGCGG CCGCTC(-3/-3)
Bst1107I 688 GTATAC GTA^TAC
BstF5I 53 CATCC GGATG(2/0)
BstYI 151 GGATCC [GA]^GATC[CT]
BstZ17I 688 GTATAC GTA^TAC
Cac8I 279 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 365 GCGC GCG^C
DpnII 152 GATC ^GATC
DraII 234 AGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 134 CGGCCG C^GGCCG
EarI 642 GAAGAG CTCTTC(1/4)
EclXI 134 CGGCCG C^GGCCG
Eco52I 134 CGGCCG C^GGCCG
EcoO109I 234 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 170 CCAAGG C^C[AT][AT]GG
FokI 53 CATCC GGATG(9/13)
HapII 149 CCGG C^CGG
HhaI 365 GCGC GCG^C
HinP1I 365 GCGC G^CGC
HincII 414 GTTGAC GT[CT]^[GA]AC
HindII 414 GTTGAC GT[CT]^[GA]AC
HindIII 768 AAGCTT A^AGCTT
HpaII 149 CCGG C^CGG
HphI 649 GGTGA GGTGA(8/7)
Ksp632I 642 GAAGAG CTCTTC(1/4)
MaeII 1042 ACGT A^CGT
MboI 152 GATC ^GATC
MfeI 887 CAATTG C^AATTG
MflI 151 GGATCC [GA]^GATC[CT]
MspI 149 CCGG C^CGG
MunI 887 CAATTG C^AATTG
NdeII 152 GATC ^GATC
NotI 133 GCGGCCGC GC^GGCCGC
NspI 861 ACATGC [GA]CATG^[CT]
PflFI 99 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI 152 GATC ^GATC
StyI 170 CCAAGG C^C[AT][AT]GG
TaqI 631 TCGA T^CGA
Tth111I 99 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 631 TCGA T^CGA
XbaI 357 TCTAGA T^CTAGA
XhoII 151 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 123 CGCG CG^CG
138 CGCG
AcsI 562 AAATTT [GA]^AATT[CT]
709 AAATTC
AlwI 152 GATCC GGATC(4/5)
151 GGATC
AlwNI 284 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
680 CAGTGACTG
ApoI 562 AAATTT [GA]^AATT[CT]
709 AAATTC
Asp700I 293 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
547 GAAGGCATTC
BbsI 874 GAAGAC GAAGAC(2/6)
27 GTCTTC
BglI 175 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
228 GCCCAAAGGGC
BpuAI 874 GAAGAC GAAGAC(2/6)
27 GTCTTC
BseRI 374 GAGGAG GAGGAG(10/8)
749 GAGGAG
BsiEI 80 CGGTCG CG[GA][CT]^CG
134 CGGCCG
BsiYI 137 CCGCGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
155 CCGAAGCCAGG
BslI 137 CCGCGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
155 CCGAAGCCAGG
BsmAI 166 GTCTC GTCTC(1/5)
906 GTCTC
BsmFI 352 GGGAC GGGAC(10/14)
840 GGGAC
BsmI 293 GAATGC GAATGC(1/-1)
551 GCATTC
BstUI 123 CGCG CG^CG
138 CGCG
DdeI 11 CTGAG C^T[ACGT]AG
965 CTGAG
DsaI 122 CCGCGG C^C[GA][CT]GG
137 CCGCGG
KspI 122 CCGCGG CCGC^GG
137 CCGCGG
MspA1I 122 CCGCGG C[AC]G^C[GT]G
137 CCGCGG
MvnI 123 CGCG CG^CG
138 CGCG
NlaIII 346 CATG CATG^
862 CATG
PleI 354 GACTC GAGTC(4/5)
967 GAGTC
PstI 71 CTGCAG CTGCA^G
116 CTGCAG
SacII 122 CCGCGG CCGC^GG
137 CCGCGG
SmlI 201 CTTGAG C^T[CT][GA]AG
910 CTTGAG
TfiI 258 GAATC G^A[AT]TC
487 GATTC
Tsp45I 682 GTGAC ^GT[GC]AC
779 GTGAC
TspRI 680 CAGTG CA[GC]TG[ACGT][ACGT]^
777 CAGTG
XcmI 170 CCAAGGCCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
452 CCATTGGAGAACTGG
XmnI 293 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
547 GAAGGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BmyI BsaJI Bsp1286I BsrDI BsrI
BssKI BstAPI BstNI Cfr13I EaeI
EcoRII Fnu4HI HaeIII HinfI ItaI
MaeI MaeIII MboII MnlI MseI
MvaI MwoI NlaIV Sau96I ScrFI
SfcI Tru9I TseI Tsp509I