Restriction map for
pFN21AE6415
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# NO CUT ENZYMES ( 139 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MaeI MamI MluI MluNI MroI
MscI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 308 GACGTC GACGT^C
AccII 925 CGCG CG^CG
Alw44I 653 GTGCAC G^TGCAC
ApaLI 653 GTGCAC G^TGCAC
AspHI 653 GTGCAC G[AT]GC[AT]^C
AvaII 964 GGTCC G^G[AT]CC
BanI 1030 GGCGCC G^G[CT][GA]CC
BbeI 1030 GGCGCC GGCGC^C
BbsI 1266 GTCTTC GAAGAC(2/6)
BbvCI 1024 GCTGAGG CCTCAGC(-5/-2)
BcnI 25 CCCGG CC^[GC]GG
BpmI 593 CTCCAG CTGGAG(16/14)
BpuAI 1266 GTCTTC GAAGAC(2/6)
BsiHKAI 653 GTGCAC G[AT]GC[AT]^C
BsmAI 497 GAGAC GTCTC(1/5)
BsrBI 910 GAGCGG CCGCTC(-3/-3)
BsrDI 1273 GCAATG GCAATG(2/0)
BsrI 1059 ACTGG ACTGG(1/-1)
BstUI 925 CGCG CG^CG
DraIII 647 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 726 GAAGAG CTCTTC(1/4)
EcoNI 672 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1030 GGCGCC [GA]GCGC^[CT]
HapII 26 CCGG C^CGG
HpaII 26 CCGG C^CGG
KasI 1030 GGCGCC G^GCGCC
Ksp632I 726 GAAGAG CTCTTC(1/4)
MfeI 1299 CAATTG C^AATTG
MspI 26 CCGG C^CGG
MunI 1299 CAATTG C^AATTG
MvnI 925 CGCG CG^CG
NarI 1030 GGCGCC GG^CGCC
NciI 25 CCCGG CC^[GC]GG
PpuMI 963 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 848 CTGCAG CTGCA^G
ScaI 1391 AGTACT AGT^ACT
SfoI 1030 GGCGCC GGC^GCC
SspI 763 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 1422 GATCC GGATC(4/5)
1295 GGATC
BglI 52 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
250 GCCCGCCTGGC
BstAPI 69 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
352 GCAGTTCTTGC
CfoI 924 GCGC GCG^C
1031 GCGC
DraII 963 GGGTCCC [GA]G^G[ACGT]CC[CT]
1253 AGGCCCT
DsaI 365 CCATGG C^C[GA][CT]GG
553 CCATGG
EcoO109I 963 GGGTCCC [GA]G^G[ACGT]CC[CT]
1253 AGGCCCT
HgaI 61 GCGTC GACGC(5/10)
425 GCGTC
HhaI 924 GCGC GCG^C
1031 GCGC
HinP1I 924 GCGC G^CGC
1031 GCGC
HphI 33 GGTGA GGTGA(8/7)
1410 GGTGA
MaeII 309 ACGT A^CGT
1103 ACGT
MspA1I 707 CAGCTG C[AC]G^C[GT]G
1226 CAGCTG
NcoI 365 CCATGG C^CATGG
553 CCATGG
PvuII 707 CAGCTG CAG^CTG
1226 CAGCTG
SfcI 488 CTGTAG C^T[GA][CT]AG
848 CTGCAG
TfiI 370 GAATC G^A[AT]TC
1340 GATTC
Tsp45I 1105 GTCAC ^GT[GC]AC
1411 GTGAC
XcmI 699 CCAAGAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1323 CCAGAGCCCCTTTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
ApoI BanII BbvI BglII BmyI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII Hin1I HindIII HinfI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII