Restriction map for
pFN21AE2229
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MamI MboII
MfeI MflI MluI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 693 GTCTAC GT^[AC][GT]AC
AccIII 108 TCCGGA T^CCGGA
AflIII 462 ACATGT A^C[GA][CT]GT
AlwI 210 GATCC GGATC(4/5)
AlwNI 345 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 320 GGGCCC GGGCC^C
BalI 128 TGGCCA TGG^CCA
BbvCI 342 CCTCAGC CCTCAGC(-5/-2)
BciVI 651 GTATCC GTATCC(6/5)
BfaI 238 CTAG C^TAG
BglI 474 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 108 TCCGGA [AT]^CCGG[AT]
BseAI 108 TCCGGA T^CCGGA
BseRI 10 GAGGAG GAGGAG(10/8)
BsgI 351 CTGCAC GTGCAG(16/14)
BsmFI 388 GGGAC GGGAC(10/14)
BspEI 108 TCCGGA T^CCGGA
BspLU11I 462 ACATGT A^CATGT
BsrDI 443 GCAATG GCAATG(2/0)
BsrI 509 ACTGG ACTGG(1/-1)
BstXI 273 CCAGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 210 GATC ^GATC
DrdI 504 GACCAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 667 CCAAGG C^C[AT][AT]GG
HaeII 338 AGCGCC [GA]GCGC^[CT]
HgaI 397 GACGC GACGC(5/10)
HphI 467 TCACC GGTGA(8/7)
MaeI 238 CTAG C^TAG
MaeII 218 ACGT A^CGT
MboI 210 GATC ^GATC
MluNI 128 TGGCCA TGG^CCA
MroI 108 TCCGGA T^CCGGA
MscI 128 TGGCCA TGG^CCA
MslI 88 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 210 GATC ^GATC
NspI 462 ACATGT [GA]CATG^[CT]
PflMI 120 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 388 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 274 CAGCTG CAG^CTG
Sau3AI 210 GATC ^GATC
SmaI 317 CCCGGG CCC^GGG
StyI 667 CCAAGG C^C[AT][AT]GG
TaqI 395 TCGA T^CGA
TthHB8I 395 TCGA T^CGA
Van91I 120 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 6 CCAGGAGGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 317 CCCGGG C^CCGGG
XmaI 317 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AfaI 141 GTAC GT^AC
701 GTAC
AvaI 28 CCCGAG C^[CT]CG[GA]G
317 CCCGGG
BanI 415 GGTGCC G^G[CT][GA]CC
435 GGTGCC
BsoBI 28 CCCGAG C^[CT]CG[GA]G
317 CCCGGG
DraII 320 GGGCCCC [GA]G^G[ACGT]CC[CT]
388 GGGACCT
EaeI 128 TGGCCA [CT]^GGCC[GA]
429 CGGCCA
EcoNI 24 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
357 CCTGGGCCAGG
EcoO109I 320 GGGCCCC [GA]G^G[ACGT]CC[CT]
388 GGGACCT
MaeIII 425 GTGAC ^GT[ACGT]AC
466 GTCAC
RsaI 141 GTAC GT^AC
701 GTAC
StuI 234 AGGCCT AGG^CCT
582 AGGCCT
Tsp45I 425 GTGAC ^GT[GC]AC
466 GTCAC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI TspRI