Restriction map for
pFN21AE1463
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MscI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 428 TCCGGA T^CCGGA
AvaI 130 CCCGAG C^[CT]CG[GA]G
BanI 315 GGCACC G^G[CT][GA]CC
BanII 152 GAGCCC G[GA]GC[CT]^C
BcnI 393 CCCGG CC^[GC]GG
BmyI 152 GAGCCC G[AGT]GC[ACT]^C
BsaWI 428 TCCGGA [AT]^CCGG[AT]
BseAI 428 TCCGGA T^CCGGA
BseRI 415 CTCCTC GAGGAG(10/8)
BsiEI 395 CGGCCG CG[GA][CT]^CG
BsoBI 130 CCCGAG C^[CT]CG[GA]G
Bsp1286I 152 GAGCCC G[AGT]GC[ACT]^C
BspEI 428 TCCGGA T^CCGGA
BspHI 222 TCATGA T^CATGA
BspMI 72 GCAGGT ACCTGC(4/8)
BsrBI 112 CCGCTC CCGCTC(-3/-3)
BsrI 21 ACTGG ACTGG(1/-1)
DdeI 307 CTCAG C^T[ACGT]AG
DpnII 78 GATC ^GATC
DraII 91 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 442 CCGTGG C^C[GA][CT]GG
EaeI 395 CGGCCG [CT]^GGCC[GA]
EagI 395 CGGCCG C^GGCCG
EclXI 395 CGGCCG C^GGCCG
Eco52I 395 CGGCCG C^GGCCG
EcoO109I 91 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 180 CCCGC CCCGC(4/6)
HaeII 357 GGCGCT [GA]GCGC^[CT]
HinfI 44 GAGTC G^A[ACGT]TC
MaeIII 31 GTCAC ^GT[ACGT]AC
MboI 78 GATC ^GATC
MboII 215 TCTTC GAAGA(8/7)
MroI 428 TCCGGA T^CCGGA
NaeI 208 GCCGGC GCC^GGC
NciI 393 CCCGG CC^[GC]GG
NdeII 78 GATC ^GATC
NgoMIV 208 GCCGGC G^CCGGC
NlaIII 223 CATG CATG^
NlaIV 315 GGCACC GG[ACGT]^[ACGT]CC
PflMI 194 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 44 GAGTC GAGTC(4/5)
RcaI 222 TCATGA T^CATGA
Sau3AI 78 GATC ^GATC
SbfI 69 CCTGCAGG CCTGCA^GG
ScaI 173 AGTACT AGT^ACT
SfaNI 212 GCATC GCATC(5/9)
Sse8387I 69 CCTGCAGG CCTGCA^GG
TaqI 137 TCGA T^CGA
Tsp45I 31 GTCAC ^GT[GC]AC
TthHB8I 137 TCGA T^CGA
Van91I 194 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AccI 145 GTCTAC GT^[AC][GT]AC
253 GTCTAC
AfaI 174 GTAC GT^AC
324 GTAC
BsiYI 150 CCGAGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
194 CCACGCTCTGG
BslI 150 CCGAGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
194 CCACGCTCTGG
BsrFI 121 ACCGGC [GA]^CCGG[CT]
208 GCCGGC
Cfr10I 121 ACCGGC [GA]^CCGG[CT]
208 GCCGGC
HgaI 432 GACGC GACGC(5/10)
143 GCGTC
MspA1I 181 CCGCTG C[AC]G^C[GT]G
319 CCGCTG
PstI 70 CTGCAG CTGCA^G
165 CTGCAG
RsaI 174 GTAC GT^AC
324 GTAC
SfcI 70 CTGCAG C^T[GA][CT]AG
165 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MnlI MspI MvaI MvnI
MwoI Sau96I ScrFI TseI