Restriction map for
pFN21AE4405
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BclI
BcnI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiHKAI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeII MamI MluI
MluNI MroI MscI MslI NarI
NciI NcoI NheI NotI NruI
NsiI NspI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AfaI 980 GTAC GT^AC
AlwI 607 GATCC GGATC(4/5)
AlwNI 124 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 155 GGGCCC GGGCC^C
Asp700I 897 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 17 CCTCAGC CCTCAGC(-5/-2)
BciVI 873 GGATAC GTATCC(6/5)
BglI 32 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1114 AGATCT A^GATCT
BsaI 887 GGTCTC GGTCTC(1/5)
BsaWI 1181 ACCGGA [AT]^CCGG[AT]
BsgI 145 GTGCAG GTGCAG(16/14)
BsiEI 276 CGGCCG CG[GA][CT]^CG
BsmAI 888 GTCTC GTCTC(1/5)
BsmI 118 GAATGC GAATGC(1/-1)
BspHI 504 TCATGA T^CATGA
BspMI 147 GCAGGT ACCTGC(4/8)
BsrBI 279 CCGCTC CCGCTC(-3/-3)
BstBI 115 TTCGAA TT^CGAA
BstYI 1114 AGATCT [GA]^GATC[CT]
CfoI 311 GCGC GCG^C
DraI 568 TTTAAA TTT^AAA
DrdI 332 GACTATCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 276 CGGCCG C^GGCCG
EclXI 276 CGGCCG C^GGCCG
Eco52I 276 CGGCCG C^GGCCG
EcoT14I 449 CCTTGG C^C[AT][AT]GG
HgaI 187 GACGC GACGC(5/10)
HhaI 311 GCGC GCG^C
HinP1I 311 GCGC G^CGC
HphI 604 GGTGA GGTGA(8/7)
KspI 34 CCGCGG CCGC^GG
MaeIII 1048 GTAAC ^GT[ACGT]AC
MfeI 479 CAATTG C^AATTG
MflI 1114 AGATCT [GA]^GATC[CT]
MunI 479 CAATTG C^AATTG
NaeI 223 GCCGGC GCC^GGC
NgoMIV 223 GCCGGC G^CCGGC
NspV 115 TTCGAA TT^CGAA
PacI 523 TTAATTAA TTAAT^TAA
PaeR7I 133 CTCGAG C^TCGAG
PflMI 702 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1038 GAGTC GAGTC(4/5)
PpuMI 298 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1185 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 504 TCATGA T^CATGA
RsaI 980 GTAC GT^AC
SacII 34 CCGCGG CCGC^GG
SapI 1004 GAAGAGC GCTCTTC(1/4)
SfcI 539 CTATAG C^T[GA][CT]AG
SfuI 115 TTCGAA TT^CGAA
SspI 597 AATATT AAT^ATT
StyI 449 CCTTGG C^C[AT][AT]GG
TspRI 1011 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 702 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 133 CTCGAG C^TCGAG
XhoII 1114 AGATCT [GA]^GATC[CT]
XmnI 897 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 299 GGACC G^G[AT]CC
1214 GGACC
BanII 30 GAGCCC G[GA]GC[CT]^C
155 GGGCCC
BbsI 1099 GAAGAC GAAGAC(2/6)
1252 GAAGAC
BfaI 833 CTAG C^TAG
1070 CTAG
BmyI 30 GAGCCC G[AGT]GC[ACT]^C
155 GGGCCC
BpuAI 1099 GAAGAC GAAGAC(2/6)
1252 GAAGAC
Bsp1286I 30 GAGCCC G[AGT]GC[ACT]^C
155 GGGCCC
BsrFI 223 GCCGGC [GA]^CCGG[CT]
1044 GCCGGT
Cfr10I 223 GCCGGC [GA]^CCGG[CT]
1044 GCCGGT
DpnII 607 GATC ^GATC
1115 GATC
DsaI 34 CCGCGG C^C[GA][CT]GG
215 CCGTGG
EaeI 200 TGGCCG [CT]^GGCC[GA]
276 CGGCCG
EarI 959 GAAGAG CTCTTC(1/4)
1004 GAAGAG
Ksp632I 959 GAAGAG CTCTTC(1/4)
1004 GAAGAG
MaeI 833 CTAG C^TAG
1070 CTAG
MboI 607 GATC ^GATC
1115 GATC
NdeI 372 CATATG CA^TATG
619 CATATG
NdeII 607 GATC ^GATC
1115 GATC
Sau3AI 607 GATC ^GATC
1115 GATC
SmlI 133 CTCGAG C^T[CT][GA]AG
821 CTCAAG
TfiI 454 GAATC G^A[AT]TC
897 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AvaI BbvI BsaJI BseRI BsiYI
BslI BsoBI BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TthHB8I