Restriction map for
pFN21AE6121
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HaeIII Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 99 CGCG CG^CG
AluI 79 AGCT AG^CT
AocI 222 CCTCAGG CC^T[ACGT]AGG
AspHI 274 GAGCAC G[AT]GC[AT]^C
BbsI 388 GTCTTC GAAGAC(2/6)
BcnI 312 CCGGG CC^[GC]GG
BlpI 162 GCTCAGC GC^T[ACGT]AGC
BmyI 274 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 162 GCTCAGC GC^T[ACGT]AGC
BpuAI 388 GTCTTC GAAGAC(2/6)
BsiHKAI 274 GAGCAC G[AT]GC[AT]^C
BsmFI 304 GTCCC GGGAC(10/14)
Bsp1286I 274 GAGCAC G[AGT]GC[ACT]^C
BstAPI 364 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 369 GGATG GGATG(2/0)
BstUI 99 CGCG CG^CG
Bsu36I 222 CCTCAGG CC^T[ACGT]AGG
CelII 162 GCTCAGC GC^T[ACGT]AGC
CfoI 100 GCGC GCG^C
DraIII 277 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 222 CCTCAGG CC^T[ACGT]AGG
FokI 369 GGATG GGATG(9/13)
HgaI 159 GACGC GACGC(5/10)
HhaI 100 GCGC GCG^C
HinP1I 100 GCGC G^CGC
HphI 153 GGTGA GGTGA(8/7)
MaeIII 259 GTAAC ^GT[ACGT]AC
MslI 147 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 99 CGCG CG^CG
NciI 312 CCGGG CC^[GC]GG
NlaIV 303 GGTCCC GG[ACGT]^[ACGT]CC
SexAI 278 ACCTGGT A^CC[AT]GGT
TaqI 128 TCGA T^CGA
TthHB8I 128 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 27 CCGC CCGC(-3/-1)
90 GCGG
AvaII 219 GGACC G^G[AT]CC
303 GGTCC
BbvI 102 GCTGC GCAGC(8/12)
105 GCTGC
BpmI 318 CTGGAG CTGGAG(16/14)
331 CTGGAG
BsgI 187 GTGCAG GTGCAG(16/14)
289 GTGCAG
BsmAI 321 GAGAC GTCTC(1/5)
34 GTCTC
BspMI 59 ACCTGC ACCTGC(4/8)
384 GCAGGT
BsrI 16 ACTGG ACTGG(1/-1)
326 ACTGG
Cac8I 63 GCAAGC GC[ACGT]^[ACGT]GC
380 GCGAGC
Cfr13I 219 GGACC G^G[ACGT]CC
303 GGTCC
DdeI 163 CTCAG C^T[ACGT]AG
223 CTCAG
DpnII 96 GATC ^GATC
126 GATC
DraII 218 AGGACCT [GA]G^G[ACGT]CC[CT]
302 AGGTCCC
EcoO109I 218 AGGACCT [GA]G^G[ACGT]CC[CT]
302 AGGTCCC
Fnu4HI 102 GCTGC GC^[ACGT]GC
105 GCTGC
HapII 115 CCGG C^CGG
312 CCGG
HpaII 115 CCGG C^CGG
312 CCGG
ItaI 102 GCTGC GC^[ACGT]GC
105 GCTGC
MboI 96 GATC ^GATC
126 GATC
MspI 115 CCGG C^CGG
312 CCGG
NdeII 96 GATC ^GATC
126 GATC
NlaIII 48 CATG CATG^
147 CATG
PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT]
302 AGGTCCC
Sau3AI 96 GATC ^GATC
126 GATC
Sau96I 219 GGACC G^G[ACGT]CC
303 GGTCC
SfaNI 342 GATGC GCATC(5/9)
370 GATGC
TseI 102 GCTGC G^C[AT]GC
105 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BsaJI BseRI BsiYI BslI BssKI
BstNI EcoRII MboII MnlI MvaI
MwoI ScrFI TspRI