Restriction map for
pFN21AE4552
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AvrII BamHI
BbeI BbrPI BbsI BcgI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I CelII ClaI
DraI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FseI
HincII HindII HpaI KasI KpnI
KspI MaeI MfeI MluI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1524 GACGTC GACGT^C
AccIII 452 TCCGGA T^CCGGA
AhdI 1111 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1597 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1959 CCTCAGG CC^T[ACGT]AGG
ApaI 1656 GGGCCC GGGCC^C
AspEI 1111 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1678 GTGCTC G[AT]GC[AT]^C
AspI 1207 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1226 TGCGCA TGC^GCA
BbvCI 1191 CCTCAGC CCTCAGC(-5/-2)
BclI 990 TGATCA T^GATCA
BglII 1809 AGATCT A^GATCT
BsaAI 1722 CACGTA [CT]AC^GT[GA]
BsaBI 369 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 452 TCCGGA [AT]^CCGG[AT]
BseAI 452 TCCGGA T^CCGGA
BsiEI 14 CGGTCG CG[GA][CT]^CG
BsiHKAI 1678 GTGCTC G[AT]GC[AT]^C
BsmI 30 GAATGC GAATGC(1/-1)
BspEI 452 TCCGGA T^CCGGA
BspHI 1199 TCATGA T^CATGA
BsrBI 1013 GAGCGG CCGCTC(-3/-3)
BsrDI 1944 CATTGC GCAATG(2/0)
BsrFI 1094 ACCGGC [GA]^CCGG[CT]
BstBI 1768 TTCGAA TT^CGAA
BstYI 1809 AGATCT [GA]^GATC[CT]
Bsu36I 1959 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1094 ACCGGC [GA]^CCGG[CT]
CpoI 625 CGGACCG CG^G[AT]CCG
Eam1105I 1111 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1959 CCTCAGG CC^T[ACGT]AGG
EcoRI 220 GAATTC G^AATTC
FauI 1866 GCGGG CCCGC(4/6)
FbaI 990 TGATCA T^GATCA
FspI 1226 TGCGCA TGC^GCA
HgaI 1540 GACGC GACGC(5/10)
HindIII 541 AAGCTT A^AGCTT
MamI 369 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1809 AGATCT [GA]^GATC[CT]
MroI 452 TCCGGA T^CCGGA
NspV 1768 TTCGAA TT^CGAA
PflFI 1207 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1199 TCATGA T^CATGA
RsrII 625 CGGACCG CG^G[AT]CCG
SbfI 1635 CCTGCAGG CCTGCA^GG
SfuI 1768 TTCGAA TT^CGAA
SmaI 434 CCCGGG CCC^GGG
Sse8387I 1635 CCTGCAGG CCTGCA^GG
StuI 1439 AGGCCT AGG^CCT
Tth111I 1207 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1809 AGATCT [GA]^GATC[CT]
XmaCI 434 CCCGGG C^CCGGG
XmaI 434 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 220 GAATTC [GA]^AATT[CT]
296 GAATTT
AcyI 1524 GACGTC G[GA]^CG[CT]C
1540 GACGCC
AlwI 1247 GGATC GGATC(4/5)
1280 GGATC
Aor51HI 179 AGCGCT AGC^GCT
594 AGCGCT
ApoI 220 GAATTC [GA]^AATT[CT]
296 GAATTT
BciVI 274 GGATAC GTATCC(6/5)
385 GGATAC
BsaHI 1524 GACGTC G[GA]^CG[CT]C
1540 GACGCC
BsaI 1052 GAGACC GGTCTC(1/5)
753 GGTCTC
BsgI 1813 CTGCAC GTGCAG(16/14)
508 GTGCAG
DsaI 1121 CCATGG C^C[GA][CT]GG
1460 CCATGG
Eco47III 179 AGCGCT AGC^GCT
594 AGCGCT
EcoNI 257 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1751 CCTATGTCAGG
EcoT14I 1121 CCATGG C^C[AT][AT]GG
1460 CCATGG
Hin1I 1524 GACGTC G[GA]^CG[CT]C
1540 GACGCC
NcoI 1121 CCATGG C^CATGG
1460 CCATGG
NspI 485 ACATGC [GA]CATG^[CT]
1028 GCATGT
PvuII 206 CAGCTG CAG^CTG
1149 CAGCTG
SmlI 776 CTCAAG C^T[CT][GA]AG
1305 CTTGAG
StyI 1121 CCATGG C^C[AT][AT]GG
1460 CCATGG
Tsp509I 221 AATT ^AATT
297 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I XcmI