################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspHI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI HgaI Hin1I HincII HindII HpaI KasI Ksp632I MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 86 GGTACC G^GTACC AccII 1086 CGCG CG^CG AflIII 709 ACATGT A^C[GA][CT]GT AhdI 334 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 86 GGTACC G^GTACC AspEI 334 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 370 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 995 GGATCC G^GATCC BbsI 483 GTCTTC GAAGAC(2/6) BciVI 934 GGATAC GTATCC(6/5) BmyI 63 GGGCAC G[AGT]GC[ACT]^C BpuAI 483 GTCTTC GAAGAC(2/6) BsaAI 475 TACGTG [CT]AC^GT[GA] BsaBI 1155 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 537 CTGCAC GTGCAG(16/14) BsiEI 1088 CGGTCG CG[GA][CT]^CG Bsp1286I 63 GGGCAC G[AGT]GC[ACT]^C BspLU11I 709 ACATGT A^CATGT BsrDI 35 GCAATG GCAATG(2/0) BsrFI 98 ACCGGC [GA]^CCGG[CT] BstAPI 471 GCATTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 954 TTCGAA TT^CGAA BstUI 1086 CGCG CG^CG BstXI 1053 CCAGAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 98 ACCGGC [GA]^CCGG[CT] Eam1105I 334 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 437 ATGCAT ATGCA^T FauI 208 CCCGC CCCGC(4/6) HaeII 926 AGCGCC [GA]GCGC^[CT] HindIII 223 AAGCTT A^AGCTT KpnI 86 GGTACC GGTAC^C KspI 1085 CCGCGG CCGC^GG MamI 1155 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 484 TCTTC GAAGA(8/7) MslI 757 CATAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1086 CGCG CG^CG NcoI 59 CCATGG C^CATGG NsiI 437 ATGCAT ATGCA^T NspI 709 ACATGT [GA]CATG^[CT] NspV 954 TTCGAA TT^CGAA PflFI 370 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 1085 CCGCGG CCGC^GG SexAI 1178 ACCTGGT A^CC[AT]GGT SfuI 954 TTCGAA TT^CGAA SmaI 542 CCCGGG CCC^GGG SmlI 595 CTCAAG C^T[CT][GA]AG Tth111I 370 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 575 CCATCACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 542 CCCGGG C^CCGGG XmaI 542 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 87 GTAC GT^AC 1101 GTAC AvaI 488 CTCGGG C^[CT]CG[GA]G 542 CCCGGG AviII 170 TGCGCA TGC^GCA 494 TGCGCA AvrII 133 CCTAGG C^CTAGG 930 CCTAGG BfaI 134 CTAG C^TAG 931 CTAG BlnI 133 CCTAGG C^CTAGG 930 CCTAGG BlpI 519 GCTGAGC GC^T[ACGT]AGC 1005 GCTCAGC Bpu1102I 519 GCTGAGC GC^T[ACGT]AGC 1005 GCTCAGC BseRI 337 GAGGAG GAGGAG(10/8) 793 GAGGAG BsmFI 602 GGGAC GGGAC(10/14) 696 GTCCC BsoBI 488 CTCGGG C^[CT]CG[GA]G 542 CCCGGG BspDI 664 ATCGAT AT^CGAT 1162 ATCGAT BspMI 89 ACCTGC ACCTGC(4/8) 167 ACCTGC CelII 519 GCTGAGC GC^T[ACGT]AGC 1005 GCTCAGC ClaI 664 ATCGAT AT^CGAT 1162 ATCGAT FspI 170 TGCGCA TGC^GCA 494 TGCGCA MaeI 134 CTAG C^TAG 931 CTAG MspA1I 655 CAGCGG C[AC]G^C[GT]G 1085 CCGCGG PpuMI 602 GGGACCT [GA]G^G[AT]CC[CT] 1013 AGGACCC RsaI 87 GTAC GT^AC 1101 GTAC TfiI 749 GAATC G^A[AT]TC 857 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BanI BbvI BcnI BpmI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MflI MluNI MnlI MscI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII