Restriction map for
pFN21AE6338
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BanI BbeI
BbrPI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI HphI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
MwoI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 640 GAATTT [GA]^AATT[CT]
AhdI 3 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 369 GTGCAC G^TGCAC
ApaLI 369 GTGCAC G^TGCAC
ApoI 640 GAATTT [GA]^AATT[CT]
AspEI 3 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 369 GTGCAC G[AT]GC[AT]^C
AvaI 695 CCCGGG C^[CT]CG[GA]G
BamHI 774 GGATCC G^GATCC
BanII 275 GGGCTC G[GA]GC[CT]^C
BbsI 801 GAAGAC GAAGAC(2/6)
BbvCI 541 CCTCAGC CCTCAGC(-5/-2)
BciVI 516 GTATCC GTATCC(6/5)
BfaI 103 CTAG C^TAG
BpmI 136 CTGGAG CTGGAG(16/14)
BpuAI 801 GAAGAC GAAGAC(2/6)
BsaWI 76 ACCGGA [AT]^CCGG[AT]
BsiHKAI 369 GTGCAC G[AT]GC[AT]^C
BsiYI 811 CCACTGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 811 CCACTGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 821 GAGAC GTCTC(1/5)
BsoBI 695 CCCGGG C^[CT]CG[GA]G
BsrDI 15 CATTGC GCAATG(2/0)
BstYI 774 GGATCC [GA]^GATC[CT]
DsaI 60 CCATGG C^C[GA][CT]GG
Eam1105I 3 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 568 GATATC GAT^ATC
EcoT14I 60 CCATGG C^C[AT][AT]GG
HaeII 112 GGCGCT [GA]GCGC^[CT]
HaeIII 855 GGCC GG^CC
HindIII 878 AAGCTT A^AGCTT
MaeI 103 CTAG C^TAG
MaeII 458 ACGT A^CGT
MflI 774 GGATCC [GA]^GATC[CT]
NcoI 60 CCATGG C^CATGG
NdeI 260 CATATG CA^TATG
NspI 600 ACATGC [GA]CATG^[CT]
PstI 739 CTGCAG CTGCA^G
SfcI 739 CTGCAG C^T[GA][CT]AG
SmaI 695 CCCGGG CCC^GGG
StyI 60 CCATGG C^C[AT][AT]GG
XhoII 774 GGATCC [GA]^GATC[CT]
XmaCI 695 CCCGGG C^CCGGG
XmaI 695 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 857 CCGC CCGC(-3/-1)
818 GCGG
AvaII 301 GGTCC G^G[AT]CC
538 GGTCC
BbvI 718 GCTGC GCAGC(8/12)
926 GCTGC
BcnI 695 CCCGG CC^[GC]GG
696 CCGGG
BmyI 275 GGGCTC G[AGT]GC[ACT]^C
369 GTGCAC
Bsp1286I 275 GGGCTC G[AGT]GC[ACT]^C
369 GTGCAC
BsrI 752 ACTGG ACTGG(1/-1)
813 ACTGG
BstNI 392 CCAGG CC^[AT]GG
769 CCTGG
CfoI 113 GCGC GCG^C
653 GCGC
Cfr13I 301 GGTCC G^G[ACGT]CC
538 GGTCC
EcoRII 392 CCAGG ^CC[AT]GG
769 CCTGG
HapII 77 CCGG C^CGG
696 CCGG
HhaI 113 GCGC GCG^C
653 GCGC
HinP1I 113 GCGC G^CGC
653 GCGC
HpaII 77 CCGG C^CGG
696 CCGG
MaeIII 11 GTCAC ^GT[ACGT]AC
200 GTGAC
MslI 61 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
622 CATTTCAATG
MspI 77 CCGG C^CGG
696 CCGG
MvaI 392 CCAGG CC^[AT]GG
769 CCTGG
NciI 695 CCCGG CC^[GC]GG
696 CCGGG
Sau96I 301 GGTCC G^G[ACGT]CC
538 GGTCC
SfaNI 474 GCATC GCATC(5/9)
765 GCATC
TseI 718 GCTGC G^C[AT]GC
926 GCTGC
Tsp45I 11 GTCAC ^GT[GC]AC
200 GTGAC
XcmI 353 CCACCTCTAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
512 CCAAGTATCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BssKI
BstF5I DdeI DpnII Fnu4HI FokI
HinfI ItaI MboI MboII MnlI
MseI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SmlI TfiI Tru9I
Tsp509I TspRI