Restriction map for
pFN21AB7152
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI Ksp632I
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccIII 158 TCCGGA T^CCGGA
AlwNI 1031 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 559 CCTGAGG CC^T[ACGT]AGG
AspHI 861 GAGCTC G[AT]GC[AT]^C
AvaI 115 CTCGGG C^[CT]CG[GA]G
BamHI 77 GGATCC G^GATCC
BbsI 337 GTCTTC GAAGAC(2/6)
BfaI 460 CTAG C^TAG
BpmI 961 CTGGAG CTGGAG(16/14)
BpuAI 337 GTCTTC GAAGAC(2/6)
BseAI 158 TCCGGA T^CCGGA
BsiHKAI 861 GAGCTC G[AT]GC[AT]^C
BsoBI 115 CTCGGG C^[CT]CG[GA]G
BspEI 158 TCCGGA T^CCGGA
BstEII 102 GGTCACC G^GT[ACGT]ACC
BstPI 102 GGTCACC G^GT[ACGT]ACC
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
DraIII 304 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
EcoNI 972 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 102 GGTCACC G^GT[ACGT]ACC
HincII 850 GTTAAC GT[CT]^[GA]AC
HindII 850 GTTAAC GT[CT]^[GA]AC
HpaI 850 GTTAAC GTT^AAC
MaeI 460 CTAG C^TAG
MaeII 905 ACGT A^CGT
MroI 158 TCCGGA T^CCGGA
PstI 1028 CTGCAG CTGCA^G
PvuII 882 CAGCTG CAG^CTG
SacI 861 GAGCTC GAGCT^C
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 527 GTAC GT^AC
828 GTAC
ApaI 395 GGGCCC GGGCC^C
968 GGGCCC
BanI 553 GGCACC G^G[CT][GA]CC
790 GGCACC
BsaWI 158 TCCGGA [AT]^CCGG[AT]
611 TCCGGT
BseRI 20 CTCCTC GAGGAG(10/8)
562 GAGGAG
BsmFI 515 GGGAC GGGAC(10/14)
713 GTCCC
BstXI 251 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
953 CCACCAGTCTGG
BstYI 77 GGATCC [GA]^GATC[CT]
680 AGATCC
Cac8I 58 GCTGGC GC[ACGT]^[ACGT]GC
632 GCTTGC
DsaI 251 CCATGG C^C[GA][CT]GG
257 CCATGG
HapII 159 CCGG C^CGG
612 CCGG
HpaII 159 CCGG C^CGG
612 CCGG
MflI 77 GGATCC [GA]^GATC[CT]
680 AGATCC
MspA1I 366 CCGCTG C[AC]G^C[GT]G
882 CAGCTG
MspI 159 CCGG C^CGG
612 CCGG
MwoI 733 GCTGTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1009 GCCAATTCTGC
NcoI 251 CCATGG C^CATGG
257 CCATGG
PpuMI 119 GGGTCCT [GA]G^G[AT]CC[CT]
722 GGGTCCT
RsaI 527 GTAC GT^AC
828 GTAC
SfaNI 384 GCATC GCATC(5/9)
541 GCATC
SfcI 494 CTACAG C^T[GA][CT]AG
1028 CTGCAG
TfiI 155 GATTC G^A[AT]TC
450 GATTC
Tsp45I 103 GTCAC ^GT[GC]AC
175 GTCAC
Tsp509I 300 AATT ^AATT
1012 AATT
XhoII 77 GGATCC [GA]^GATC[CT]
680 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MvaI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI StyI Tru9I
TseI TspRI