Restriction map for
pFN21AE4336
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HindIII KasI
KpnI KspI MamI MfeI MflI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 100 GTCTAC GT^[AC][GT]AC
AccII 105 CGCG CG^CG
AccIII 306 TCCGGA T^CCGGA
AcyI 616 GACGCC G[GA]^CG[CT]C
Aor51HI 62 AGCGCT AGC^GCT
AvaI 276 CTCGGG C^[CT]CG[GA]G
BalI 927 TGGCCA TGG^CCA
BcgI 135 CGACGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 594 CCGGG CC^[GC]GG
BfaI 871 CTAG C^TAG
BsaHI 616 GACGCC G[GA]^CG[CT]C
BsaI 572 GAGACC GGTCTC(1/5)
BsaWI 306 TCCGGA [AT]^CCGG[AT]
BseAI 306 TCCGGA T^CCGGA
BseRI 502 GAGGAG GAGGAG(10/8)
BsgI 540 CTGCAC GTGCAG(16/14)
BsmAI 572 GAGAC GTCTC(1/5)
BsmFI 694 GGGAC GGGAC(10/14)
BsoBI 276 CTCGGG C^[CT]CG[GA]G
BspEI 306 TCCGGA T^CCGGA
BspHI 605 TCATGA T^CATGA
BsrFI 145 GCCGGC [GA]^CCGG[CT]
BstUI 105 CGCG CG^CG
Cac8I 145 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 145 GCCGGC [GA]^CCGG[CT]
CpoI 107 CGGACCG CG^G[AT]CCG
DpnII 603 GATC ^GATC
DraII 694 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 930 CCATGG C^C[GA][CT]GG
EarI 289 CTCTTC CTCTTC(1/4)
Eco47III 62 AGCGCT AGC^GCT
EcoO109I 694 GGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 616 GACGC GACGC(5/10)
Hin1I 616 GACGCC G[GA]^CG[CT]C
HpaI 453 GTTAAC GTT^AAC
Ksp632I 289 CTCTTC CTCTTC(1/4)
MaeI 871 CTAG C^TAG
MaeIII 706 GTTAC ^GT[ACGT]AC
MboI 603 GATC ^GATC
MluNI 927 TGGCCA TGG^CCA
MroI 306 TCCGGA T^CCGGA
MscI 927 TGGCCA TGG^CCA
MseI 454 TTAA T^TAA
MspA1I 481 CAGCGG C[AC]G^C[GT]G
MvnI 105 CGCG CG^CG
NaeI 145 GCCGGC GCC^GGC
NciI 594 CCGGG CC^[GC]GG
NcoI 930 CCATGG C^CATGG
NdeII 603 GATC ^GATC
NgoMIV 145 GCCGGC G^CCGGC
PflMI 950 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 694 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 605 TCATGA T^CATGA
RsrII 107 CGGACCG CG^G[AT]CCG
Sau3AI 603 GATC ^GATC
Tru9I 454 TTAA T^TAA
Van91I 950 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 128 CCACCACCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 681 GTAC GT^AC
853 GTAC
AlwNI 82 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
436 CAGTGGCTG
BanII 799 GAGCCC G[GA]GC[CT]^C
943 GGGCTC
BglI 929 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
967 GCCCTGGAGGC
BsmI 813 GAATGC GAATGC(1/-1)
1022 GAATGC
DrdI 91 GACATAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
526 GACGTGATTGTC
EaeI 847 CGGCCA [CT]^GGCC[GA]
927 TGGCCA
EcoT14I 622 CCTTGG C^C[AT][AT]GG
930 CCATGG
HaeII 62 AGCGCT [GA]GCGC^[CT]
561 GGCGCT
HincII 453 GTTAAC GT[CT]^[GA]AC
535 GTCAAC
HindII 453 GTTAAC GT[CT]^[GA]AC
535 GTCAAC
MaeII 137 ACGT A^CGT
527 ACGT
MwoI 929 GCCATGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
967 GCCCTGGAGGC
PleI 26 GAGTC GAGTC(4/5)
493 GAGTC
RsaI 681 GTAC GT^AC
853 GTAC
StyI 622 CCTTGG C^C[AT][AT]GG
930 CCATGG
TfiI 864 GATTC G^A[AT]TC
995 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI Asp700I AvaII BbvI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboII MnlI MslI MspI MvaI
NlaIII NlaIV PstI Sau96I ScrFI
SfaNI SfcI TseI Tsp509I TspRI
XmnI