Restriction map for
pFN21AE3908
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# NO CUT ENZYMES ( 130 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbsI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MroI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 244 GACGTC GACGT^C
AccI 661 GTAGAC GT^[AC][GT]AC
AlwNI 1136 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1369 CCTGAGG CC^T[ACGT]AGG
Asp700I 7 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 252 GAGCAC G[AT]GC[AT]^C
BalI 91 TGGCCA TGG^CCA
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
BglI 386 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 26 CTGGAG CTGGAG(16/14)
BsaBI 554 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1053 CTGCAC GTGCAG(16/14)
BsiHKAI 252 GAGCAC G[AT]GC[AT]^C
BsmFI 400 GGGAC GGGAC(10/14)
BsmI 1470 GAATGC GAATGC(1/-1)
Bsp1407I 992 TGTACA T^GTACA
BspLU11I 995 ACATGT A^CATGT
BspMI 546 GCAGGT ACCTGC(4/8)
BsrGI 992 TGTACA T^GTACA
BstAPI 212 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 553 GGATCT [GA]^GATC[CT]
Bsu36I 1369 CCTGAGG CC^T[ACGT]AGG
DsaI 1112 CCGTGG C^C[GA][CT]GG
Eco81I 1369 CCTGAGG CC^T[ACGT]AGG
EcoRI 1525 GAATTC G^AATTC
HgaI 975 GACGC GACGC(5/10)
MamI 554 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 553 GGATCT [GA]^GATC[CT]
MluNI 91 TGGCCA TGG^CCA
MscI 91 TGGCCA TGG^CCA
MspA1I 388 CAGCTG C[AC]G^C[GT]G
PflMI 486 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1178 CTGCAG CTGCA^G
PvuII 388 CAGCTG CAG^CTG
SmlI 1234 CTCAAG C^T[CT][GA]AG
SspBI 992 TGTACA T^GTACA
Van91I 486 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 387 CCAGCTGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 553 GGATCT [GA]^GATC[CT]
XmnI 7 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AvaII 322 GGTCC G^G[AT]CC
1593 GGACC
BanI 844 GGCGCC G^G[CT][GA]CC
1100 GGCGCC
BbeI 844 GGCGCC GGCGC^C
1100 GGCGCC
BbrPI 988 CACGTG CAC^GTG
1095 CACGTG
BcgI 1062 CGACGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1464 CGAGGAGAATGC
BfaI 1195 CTAG C^TAG
1390 CTAG
BsaAI 988 CACGTG [CT]AC^GT[GA]
1095 CACGTG
BsmAI 29 GAGAC GTCTC(1/5)
270 GAGAC
BsrDI 164 GCAATG GCAATG(2/0)
395 GCAATG
BstF5I 768 CATCC GGATG(2/0)
1281 GGATG
CfoI 845 GCGC GCG^C
1101 GCGC
DraI 282 TTTAAA TTT^AAA
1645 TTTAAA
EaeI 91 TGGCCA [CT]^GGCC[GA]
968 CGGCCA
EarI 587 GAAGAG CTCTTC(1/4)
1152 GAAGAG
FauI 1032 CCCGC CCCGC(4/6)
1087 CCCGC
FokI 768 CATCC GGATG(9/13)
1281 GGATG
HaeII 844 GGCGCC [GA]GCGC^[CT]
1100 GGCGCC
HhaI 845 GCGC GCG^C
1101 GCGC
HinP1I 845 GCGC G^CGC
1101 GCGC
KasI 844 GGCGCC G^GCGCC
1100 GGCGCC
Ksp632I 587 GAAGAG CTCTTC(1/4)
1152 GAAGAG
MaeI 1195 CTAG C^TAG
1390 CTAG
NarI 844 GGCGCC GG^CGCC
1100 GGCGCC
NspI 631 GCATGT [GA]CATG^[CT]
995 ACATGT
PmaCI 988 CACGTG CAC^GTG
1095 CACGTG
PmlI 988 CACGTG CAC^GTG
1095 CACGTG
SfoI 844 GGCGCC GGC^GCC
1100 GGCGCC
TfiI 277 GATTC G^A[AT]TC
1239 GATTC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII Hin1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I