Restriction map for
pFN21AE3916
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbeI BciVI
BclI BglI BlnI BsaHI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
DsaI Eam1105I EarI Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 604 CGCG CG^CG
AflII 1315 CTTAAG C^TTAAG
Aor51HI 431 AGCGCT AGC^GCT
AvaI 403 CTCGAG C^[CT]CG[GA]G
AviII 1262 TGCGCA TGC^GCA
BbrPI 964 CACGTG CAC^GTG
BbsI 340 GAAGAC GAAGAC(2/6)
BfaI 796 CTAG C^TAG
BfrI 1315 CTTAAG C^TTAAG
BpuAI 340 GAAGAC GAAGAC(2/6)
BsaAI 964 CACGTG [CT]AC^GT[GA]
BsaBI 1206 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 841 GAGACC GGTCTC(1/5)
BsgI 556 CTGCAC GTGCAG(16/14)
BsiEI 418 CGGCCG CG[GA][CT]^CG
BsoBI 403 CTCGAG C^[CT]CG[GA]G
BsrBI 1279 GAGCGG CCGCTC(-3/-3)
BsrDI 780 CATTGC GCAATG(2/0)
BstAPI 477 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 604 CGCG CG^CG
BstXI 935 CCAGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 961 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 418 CGGCCG C^GGCCG
EclXI 418 CGGCCG C^GGCCG
Eco47III 431 AGCGCT AGC^GCT
Eco52I 418 CGGCCG C^GGCCG
EcoT14I 1094 CCTTGG C^C[AT][AT]GG
FspI 1262 TGCGCA TGC^GCA
HindIII 5 AAGCTT A^AGCTT
HinfI 376 GAGTC G^A[ACGT]TC
MaeI 796 CTAG C^TAG
MaeII 965 ACGT A^CGT
MamI 1206 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1316 TTAA T^TAA
MvnI 604 CGCG CG^CG
PaeR7I 403 CTCGAG C^TCGAG
PleI 376 GAGTC GAGTC(4/5)
PmaCI 964 CACGTG CAC^GTG
PmlI 964 CACGTG CAC^GTG
PpuMI 911 AGGACCT [GA]G^G[AT]CC[CT]
SacI 469 GAGCTC GAGCT^C
SexAI 47 ACCAGGT A^CC[AT]GGT
StuI 257 AGGCCT AGG^CCT
StyI 1094 CCTTGG C^C[AT][AT]GG
Tru9I 1316 TTAA T^TAA
XcmI 779 CCATTGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 403 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 21 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1230 GAACCCCTTC
BanI 321 GGTGCC G^G[CT][GA]CC
821 GGTGCC
BanII 282 GAGCCC G[GA]GC[CT]^C
469 GAGCTC
BcgI 606 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1365 CGAGGGCTGTGC
BglII 59 AGATCT A^GATCT
1124 AGATCT
BsaWI 11 ACCGGA [AT]^CCGG[AT]
644 ACCGGA
BsmAI 841 GAGAC GTCTC(1/5)
1019 GTCTC
BsmFI 185 GGGAC GGGAC(10/14)
1069 GGGAC
BsrFI 154 GCCGGT [GA]^CCGG[CT]
509 ACCGGC
BsrI 992 ACTGG ACTGG(1/-1)
1158 CCAGT
BssSI 515 CTCGTG CACGAG(-5/-1)
1000 CTCGTG
Cfr10I 154 GCCGGT [GA]^CCGG[CT]
509 ACCGGC
EcoNI 795 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1059 CCTGTACGAGG
HaeII 431 AGCGCT [GA]GCGC^[CT]
811 AGCGCC
MboII 340 GAAGA GAAGA(8/7)
1172 TCTTC
MslI 236 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
538 CACCTCCGTG
PflMI 366 CCAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACCTGCTGG
PstI 169 CTGCAG CTGCA^G
238 CTGCAG
PvuII 1249 CAGCTG CAG^CTG
1258 CAGCTG
TaqI 275 TCGA T^CGA
404 TCGA
TspRI 236 CACTG CA[GC]TG[ACGT][ACGT]^
241 CAGTG
TthHB8I 275 TCGA T^CGA
404 TCGA
Van91I 366 CCAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACCTGCTGG
XmnI 21 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1230 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BbvCI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BspMI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MflI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I XhoII